Effect of dietary changes on the bacteriophage population in the rumen of sheep

Item does not contain fulltext4th Symposium on Gut Microbiology, 21 juni 200

Identification of mitochondrial-type chaperonin 60 (HSP 60) proteins in the anaerobic ciliate Nyctotherus ovalis

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Identification of a “bacterial” gene in Nyctotherus ovalis

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Identification of a PPi-dependent Phosphofructokinase from the anaerobic ciliate Nyctotherus ovalis

Item does not contain fulltext4th Symposium on Gut Microbiology, 21 juni 200

Reconciling morphological and 18S rRNA phylogenies in the stichotrines (Ciliophoa, Spirotrichea), including new sequences from some rare species

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Diversity of rumen ciliates in a red deer assessed from molecule and morphology

Item does not contain fulltextJahrestagung der DGP, 3 maart 200

Reconciling classical and molecular phylogenies in the stichotrichines (Ciliophora, Spirotrichea), including new sequences from some rare species

Item does not contain fulltextWe performed a comparative morphological and molecular study on oxytrichid and urostylid stichotrichs (= part of the former hypotrichs). Included are new small subunit (18S) ribosomal RNA (rRNA) gene sequences from five rare oxytrichids (Gonostomum namibiense, Cyrtohymena citrina, Hemiurosoma terricola, Onychodromopsis flexilis, Orthoamphisiella breviseries) and published sequences, based on cultures provided by the senior author, of two key stichotrichid genera, viz., Gastrostyla and Engelmanniella. These and other sequences, altogether 27 species representing 23 genera, were used to analyze how 18S rRNA-based phylogenetic trees can be reconciled with the morphological and ontogenetical data. In 18S rRNA trees, the oligotrichine family Halteriidae invariably clusters within the oxytrichid clade, usually near Oxytricha granulifera, type species of the genus. This position is hardly supported by morphological and ecological evidence and, especially, it contradicts the current ontogenetic findings; possibly, it is an artifact caused by taxa undersampling and/or special molecular evolutionary events. In contrast, most morphological and DNA sequence data of the stichotrichs can be harmonized with the CEUU (Convergent Evolution of Urostylids and Uroleptids) hypothesis which suggests that the urostylid midventral pattern evolved from an oxytrichine ancestor, developing a second time within the Oxytrichidae. The systematic position of one of the two key genera could be clarified with the 18S rRNA sequences: Gastrostyla is a stylonychine oxytrichid. Based on the molecular data and a reassessment of ontogenesis, a new genus, Styxophrya nov. gen., is established for Onychodromus quadricornutus Foissner, Schlegel & Prescott, 1987. (C) 2004 Elsevier GmbH. All rights reserved

Diversity of rumen ciliates in a red deer assessed from 18S rDNA and morphology

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Gene-sized macronuclear chromosomes in the anaerobic ciliate Nyctotherus ovalis

Item does not contain fulltext4th Symposium on Gut Microbiology, 21 juni 200

Eukaryotic diversity in historical soil samples

The eukaryotic biodiversity in historical air-dried samples of Dutch agricultural soil has been assessed by random sequencing of an 18S rRNA gene library and by denaturing gradient gel electrophoresis. Representatives of nearly all taxa of eukaryotic soil microbes could be identified, demonstrating that it is possible to study eukaryotic microbiota in samples from soil archives that have been stored for more than 30 years at room temperature. In a pilot study, 41 sequences were retrieved that could be assigned to fungi and a variety of aerobic and anaerobic protists such as cercozoans, ciliates, xanthophytes (stramenopiles), heteroloboseans, and amoebozoans. A PCR-denaturing gradient gel electrophoresis analysis of samples collected between 1950 and 1975 revealed significant changes in the composition of the eukaryotic microbiota