On-Farm Flood Capture and Recharge (OFFCR) at an Organic Almond Orchard, Recharge Rates and Soil Profile Responses Groundwater Recharge Project, 2016

Groundwater in much of California’s Central Valley (CV) has been critically over-drafted resulting in the implementation of the 2014 Sustainable Groundwater Management Act (SGMA). As Groundwater Sustainability Agencies (GSAs) work to comply with SGMA requirements and timelines, On-Farm Floodwater Capture and Recharge (OFFCR) is being studied to help increase recharge capacity. We implemented an OFFCR test on an organic almond orchard in the CV to assess achievable recharge rates attained through over-irrigation, and potential soil and water quality impacts. Irrigation water was applied via flood irrigation. We developed study sites and installed soil sensors for moisture and salinity monitoring, took post-irrigation deep cores to assess changes in soil and porewater nitrogen and salt concentrations through the vadose zone, and monitored agronomic practices, recharge loading and crop yields. These studies were conducted on three recharge treatments with three replicated stations for each: 1) Control at about 6 inches of flooded water to meet ET as typical for irrigation (Control treatment), 2) Low Flooding of about 12 inches per irrigation application (Mid treatment), and 3) High Flooding of about 24 inches per irrigation application (High treatment)

The amyloid precursor protein controls PIKfyve function

While the Amyloid Precursor Protein (APP) plays a central role in Alzheimer's disease, its cellular function still remains largely unclear. It was our goal to establish APP function which will provide insights into APP's implication in Alzheimer's disease. Using our recently developed proteo-liposome assay we established the interactome of APP's intracellular domain (known as AICD), thereby identifying novel APP interactors that provide mechanistic insights into APP function. By combining biochemical, cell biological and genetic approaches we validated the functional significance of one of these novel interactors. Here we show that APP binds the PIKfyve complex, an essential kinase for the synthesis of the endosomal phosphoinositide phosphatidylinositol-3,5-bisphosphate. This signalling lipid plays a crucial role in endosomal homeostasis and receptor sorting. Loss of PIKfyve function by mutation causes profound neurodegeneration in mammals. Using C. elegans genetics we demonstrate that APP functionally cooperates with PIKfyve in vivo. This regulation is required for maintaining endosomal and neuronal function. Our findings establish an unexpected role for APP in the regulation of endosomal phosphoinositide metabolism with dramatic consequences for endosomal biology and important implications for our understanding of Alzheimer's disease

Convalescent plasma in patients admitted to hospital with COVID-19 (RECOVERY): a randomised controlled, open-label, platform trial

Background: Many patients with COVID-19 have been treated with plasma containing anti-SARS-CoV-2 antibodies. We aimed to evaluate the safety and efficacy of convalescent plasma therapy in patients admitted to hospital with COVID-19. Methods: This randomised, controlled, open-label, platform trial (Randomised Evaluation of COVID-19 Therapy [RECOVERY]) is assessing several possible treatments in patients hospitalised with COVID-19 in the UK. The trial is underway at 177 NHS hospitals from across the UK. Eligible and consenting patients were randomly assigned (1:1) to receive either usual care alone (usual care group) or usual care plus high-titre convalescent plasma (convalescent plasma group). The primary outcome was 28-day mortality, analysed on an intention-to-treat basis. The trial is registered with ISRCTN, 50189673, and ClinicalTrials.gov, NCT04381936. Findings: Between May 28, 2020, and Jan 15, 2021, 11558 (71%) of 16287 patients enrolled in RECOVERY were eligible to receive convalescent plasma and were assigned to either the convalescent plasma group or the usual care group. There was no significant difference in 28-day mortality between the two groups: 1399 (24%) of 5795 patients in the convalescent plasma group and 1408 (24%) of 5763 patients in the usual care group died within 28 days (rate ratio 1·00, 95% CI 0·93–1·07; p=0·95). The 28-day mortality rate ratio was similar in all prespecified subgroups of patients, including in those patients without detectable SARS-CoV-2 antibodies at randomisation. Allocation to convalescent plasma had no significant effect on the proportion of patients discharged from hospital within 28 days (3832 [66%] patients in the convalescent plasma group vs 3822 [66%] patients in the usual care group; rate ratio 0·99, 95% CI 0·94–1·03; p=0·57). Among those not on invasive mechanical ventilation at randomisation, there was no significant difference in the proportion of patients meeting the composite endpoint of progression to invasive mechanical ventilation or death (1568 [29%] of 5493 patients in the convalescent plasma group vs 1568 [29%] of 5448 patients in the usual care group; rate ratio 0·99, 95% CI 0·93–1·05; p=0·79). Interpretation: In patients hospitalised with COVID-19, high-titre convalescent plasma did not improve survival or other prespecified clinical outcomes. Funding: UK Research and Innovation (Medical Research Council) and National Institute of Health Research

Sierra Valley, CA – A white paper on the opportunities and challenges for management of groundwater under SGMA

This paper discusses groundwater sustainability in California’s Sierra Valley based upon review of various hydrologic and geologic data sets and publications and presents our findings in the context of the 2014 Sustainable Groundwater Management Act (SGMA). The discussion related to SGMA is based upon our current understanding of the legislation. As this legislation is implemented, its interpretation may evolve. The paper provides potential next steps and mitigation strategies as Sierra Valley works to move toward sustainable groundwater management

Groundwater relationships to pumping, precipitation and geology in high-elevation basin, Sierra Valley, CA

Sierra Valley, located in the northern Sierra Nevada, California, serves as the Middle Fork Feather River headwaters and provides surface water to Oroville Dam of the California State Water Project (SWP). Under California’s Sustainable Groundwater Management Act (SGMA), the Sierra Valley sub-basin has been designated a medium-priority basin, due to chronic groundwater declines and the valley’s high ecological value as the largest freshwater marsh and meadow system in the Sierra Nevada. The Sierra Valley Groundwater Management District (SVGMD) serves as the Groundwater Sustainability Agency (GSA) for the Sierra Valley sub-basin. As such, SVGMD is tasked through SMGA with achieving sustainable groundwater management over an approximate 20-y timeframe. The first step is the development of a Groundwater Sustainability Plan (GSP) (to be completed by January 2022) that 1) hydrologically assesses the basin, 2) identifies methods and protocols to track groundwater trends, and 3) develops an initial suite of actions to move the basin towards groundwater sustainability. ... Our investigation builds on previous watershed studies and further establishes the Sierra Valley watershed as a highly complex hydrologic system. These complexities include: large variation in precipitation phase and quantity throughout the watershed; geologic features that restrict both vertical and lateral groundwater flow; many water inflow pathways, both surface and sub-surface, that are logistically impossible to quantify by conventional monitoring means. Prior attempts at developing accurate water budgets and numerical models of the watershed have been hindered by the uncertainty these factors present. Thus, though a hydrologic budget is required by SGMA for the development of the GSP, numerical models will be of limited utility as either tools to derive hydrologic budgets or to help determine the efficacy management actions to achieve sustainable groundwater conditions. In developing strategies to address undesirable groundwater conditions, we recommend an adaptive management approach paired with targeted and defensible data collection with standardized data collection, management and quality control procedures