Multiple traces of monkeypox detected in non-sewered wastewater with sparse sampling from a densely populated metropolitan area in Asia

The monkeypox virus is excreted in the feces of infected individuals. Therefore, there is an interest in using viral load detection in wastewater for sentinel early surveillance at a community level and as a complementary approach to syndromic surveillance. We collected wastewater from 63 sewered and non-sewered locations in Bangkok city center between May and August 2022. Monkeypox viral DNA copy numbers were quantified using real-time polymerase chain reaction (PCR) and confirmed positive by Sanger sequencing. Monkeypox viral DNA was first detected in wastewater from the second week of June 2022, with a mean copy number of 16.4 copies/ml (n = 3). From the first week of July, the number of viral DNA copies increased to a mean copy number of 45.92 copies/ml. Positive samples were Sanger sequenced and confirmed the presence of the monkeypox virus. Our study is the first to detect monkeypox viral DNA in wastewater from various locations within Thailand. Results suggest that this could be a complementary source for detecting viral DNA and predicting upcoming outbreaks

A Quarter of Century in Artificial Intelligence and Law: Projects, Personal Trajectories, a Subjective PerspectiveLanguage, Culture, Computation. Computing of the Humanities, Law, and Narratives

This article describes projects in the domain of artificial intelligence and law, which resulted from the research of the five authors listed, when they formed teams (of the first author named and each one of the other authors). Therefore, the present paper offers a subjective perspective, from the viewpoint of personal trajectories within AI & Law. Several, though not all, of the projects concerned dealt with facets of legal evidence. These projects include: ALIBI (an AI planner generating exonerating accounts); a representation of Italy’s regional constitutions in a nested-relation representation (a precursor of XML); the application of kappa calculus and a probabilistic interpretation to a Scandinavian approach to evidential strength; the application of Petri Nets for representing temporal relations in mutual wills; Daedalus (Judge Asaro’s software assisting Italy’s examining magistrates with inquiries, and then when they turn prosecutors); a study in occurrences in court of allegations echoing the pretext archetype “The dog ate my homework” (even when the claim was not pretextuous); an application of Wigmore Charts to an analysis of both the argumentation and the rhetoric of an Italian arringa (final submissions to the court) from a real court case; editorial projects which promoted the emergence of evidence as a conspicuous field within AI & Law (thus overturning previous neglect); and a magnum opus (Nissan 2012a) which presents the state of the art of computational applications to legal evidence, police inquiries, or argumentation

The ASOS Surgical Risk Calculator: development and validation of a tool for identifying African surgical patients at risk of severe postoperative complications

Background: The African Surgical Outcomes Study (ASOS) showed that surgical patients in Africa have a mortality twice the global average. Existing risk assessment tools are not valid for use in this population because the pattern of risk for poor outcomes differs from high-income countries. The objective of this study was to derive and validate a simple, preoperative risk stratification tool to identify African surgical patients at risk for in-hospital postoperative mortality and severe complications. Methods: ASOS was a 7-day prospective cohort study of adult patients undergoing surgery in Africa. The ASOS Surgical Risk Calculator was constructed with a multivariable logistic regression model for the outcome of in-hospital mortality and severe postoperative complications. The following preoperative risk factors were entered into the model; age, sex, smoking status, ASA physical status, preoperative chronic comorbid conditions, indication for surgery, urgency, severity, and type of surgery. Results: The model was derived from 8799 patients from 168 African hospitals. The composite outcome of severe postoperative complications and death occurred in 423/8799 (4.8%) patients. The ASOS Surgical Risk Calculator includes the following risk factors: age, ASA physical status, indication for surgery, urgency, severity, and type of surgery. The model showed good discrimination with an area under the receiver operating characteristic curve of 0.805 and good calibration with c-statistic corrected for optimism of 0.784. Conclusions: This simple preoperative risk calculator could be used to identify high-risk surgical patients in African hospitals and facilitate increased postoperative surveillance. © 2018 British Journal of Anaesthesia. Published by Elsevier Ltd. All rights reserved.Medical Research Council of South Africa gran