Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia

The current classification of the rhizobia (root-nodule symbionts) assigns them to six genera. It is strongly influenced by the small subunit (16S, SSU) rRNA molecular phylogeny, but such single-gene phylogenies may not reflect the evolution of the genome as a whole. To test this, parts of the atpD and recA genes have been sequenced for 25 type strains within the alpha -Proteobacteria, representing species in Rhizobium, Sinorhizobium, Mesorhizobium, Bradyrhizobium, Azorhizobium, Agrobacterium, Phyllobacterium, Mycoplana and Brevundimonas. The current genera Sinorhizobium and Mesorhizobium are well supported by these genes, each forming a distinct phylogenetic clade with unequivocal bootstrap support. There is good support for a Rhizobium clade that includes Agrobacterium tumefaciens, and the very close relationship between Agrobacterium rhizogenes and Rhizobium tropici is confirmed. There is evidence for recombination within the genera Mesorhizobium and Sinorhizobium, but the congruence of the phylogenies at higher levels indicates that the genera are genetically isolated. rRNA provides a reliable distinction between genera, but genetic relationships within a genus may be disturbed by recombination

Sustained pupillary constrictions mediated by an L- and M-cone opponent process

AbstractWe show that irradiance-coding alone cannot explain the sustained pupillary constrictions evoked by chromatic and luminance variations in a stimulus. For example, stimulus modulations that decremented the contrasts in L- and M-cones as well as rods and melanopsin photoreceptors produced sustained constrictions rather than the predicted dilations. Although the sustained responses are unidirectional, we confirm that they are at least partially mediated by an L- and M-cone opponent interaction. We discuss the implications of sustained unidirectional chromatic responses in view of the function of the pupil to improve the clarity of vision

Pupillary correlates of light-evoked melanopsin activity in humans

AbstractWe investigated whether cones are the only photosensitive process mediating the photopic pupillary light reflex. New analyses were performed on previously published recordings, focusing on those evoked by the onset of photopically equated short- and long-wavelength stimuli. Comparisons between responses revealed contraction differences that slowly grew to a peak and gradually declined. The late contraction was associated with short wavelengths and appeared mostly at the higher stimulus intensities. We conclude that cones are not the only photoreception process mediating the photopic ON-reflex and infer that melanopsin is another. Melanopsin contributes to the steady-state pupil size in daylight illumination

Intensified array camera imaging of solid surface combustion aboard the NASA Learjet

An intensified array camera was used to image weakly luminous flames spreading over thermally thin paper samples in a low gravity environment aboard the NASA-Lewis Learjet. The aircraft offers 10 to 20 sec of reduced gravity during execution of a Keplerian trajectory and allows the use of instrumentation that is delicate or requires higher electrical power than is available in drop towers. The intensified array camera is a charge intensified device type that responds to light between 400 and 900 nm and has a minimum sensitivity of 10(exp 6) footcandles. The paper sample, either ashless filter paper or a lab wiper, burns inside a sealed chamber which is filled with 21, 18, or 15 pct. oxygen in nitrogen at one atmosphere. The camera views the edge of the paper and its output is recorded on videotape. Flame positions are measured every 0.1 sec to calculate flame spread rates. Comparisons with drop tower data indicate that the flame shapes and spread rates are affected by the residual g level in the aircraft

The common nodulation genes of Astragalus sinicus rhizobia are conserved despite chromosomal diversity

The nodulation genes of Mesorhizobium sp. (Astragalus sinicus) strain 7653R were cloned by functional complementation of Sinorhizobium meliloti nod mutants. The common nod genes, nodD, nodA, and nodBC, were identified by heterologous hybridization and sequence analysis. The nodA gene was found to be separated from nodBC by approximately 22 kb and was divergently transcribed. The 2.0-kb nodDBC region was amplified by PCR from 24 rhizobial strains nodulating A. sinicus, which represented different chromosomal genotypes and geographic origins. No polymorphism was found in the size of PCR products, suggesting that the separation of nodA from nodBC is a common feature of A. sinicus rhizobia. Sequence analysis of the PCR-amplified nodA gene indicated that seven strains representing different 16S and 23S ribosomal DNA genotypes had identical nodA sequences. These data indicate that, whereas microsymbionts of A. sinicus exhibit chromosomal diversity, their nodulation genes are conserved, supporting the hypothesis of horizontal transfer of nod genes among diverse recipient bacteria

A pupillometric correlate of scotopic visual acuity

AbstractWhile not easily fit into the classic descriptions of the pupillary light reflex, previous studies reported that changes in the spatial composition of the retinal image can evoke a pupillary response. The present study extends this observation by showing that the pupil constricts in response to scotopic as well as photopic spatial patterns. Moreover, the amplitude of the scotopic response decreases with increasing spatial frequencies suggesting a pupillary spatial acuity of about 3 c/deg. The scotopic pupil acuity is similar to the scotopic perceptual visual acuity measured in the same observers

Torque magnetometry of an amorphous-alumina/strontium-titanate interface

We report torque magnetometry measurements of an oxide heterostructure consisting of an amorphous Al2O3 thin film grown on a crystalline SrTiO3 substrate (a-AO/STO) by atomic layer deposition. We find a torque response that resembles previous studies of crystalline LaAlO3/SrTiO3 (LAO/STO) heterointerfaces, consistent with strongly anisotropic magnetic ordering in the plane of the interface. Unlike crystalline LAO, amorphous Al2O3 is nonpolar, indicating that planar magnetism at an oxide interface is possible without the strong internal electric fields generated within the polarization catastrophe model. We discuss our results in the context of current theoretical efforts to explain magnetism in crystalline LAO/STO.Chemistry and Chemical Biolog