Molecular characterisation, expression and localisation of human neurokinin-3 receptor

AbstractThe complete amino acid sequence of the human neurokinin-3 receptor was deduced by DNA sequence analysis of human genomic fragments. Comparison of the predicted primary structure with those for the human neurokinin receptors 1 and 2 shows a highly conserved pattern of seven hydrophobic regions with maximum divergence occuring at the amino- and carboxy-termini. The position of intron-exon junctions are identical to those in other reported neurokinin genes. Using a chimeric genomic-cDNA gene, the human NK-3 receptor was expressed in Xenopus laevis oocytes where it mediates membrane conductance changes in response to its agonist, neurokinin B. More significantly, expression of the gene in mammalian cells resulted in detection of receptor binding as well as neurokinin-stimulated calcium mobilization and arachidonic acid release, all displaying the pharmacological characteristics expected of a neurokinin-3 receptor, By using the polymerase chain reaction we have shown that mRNA for the human neurokinin-3 receptor is expressed predominantly in the central nervous system

AHA-GRAPE: Adaptive Hydrodynamic Architecture – GRAvity PipE

In astrophysics numerical star cluster simulations and hydrodynamical methods like SPH require computational performance in the petaflop range. The GRAPE family of ASIC-based accelerators improves the cost-performance ratio compared to general purpose parallel computers, however with limited flexibility. The AHA-GRAPE architecture adds a reconfigurable FPGA-processor to accelerate the SPH computation. The basic equations of the algorithm consist of three parts each scaling with the order of O(N), O(N*Nn), and O(N**2) respectively, where N is in the range of 10**4 to 10**7 and Nn approximately 50. These equations can profitably be distributed across a host workstation, an FPGA processor, and a GRAPE subsystem. With the new ATLANTIS FPGA processor we expect a scalable SPH performance of 1.5 Gflops per board. The first prototype AHA-GRAPE system will be available in mid-2000. This 3-layered system will deliver an increase in performance by a factor of 10 as compared to a pure GRAPE solution.Comment: 7 pages, 3 figures, to appear in Procs. of The 1999 International Conference on Parallel and Distributed Processing Techniques and Applications (PDPTA 99), Las Vegas, US

AN EVALUATION OF FURTHER REFORMS IN THE DEMOCRATIC PARTY'S PRESIDENTIAL NOMINATION PROCESS

By 1992, the party of the majority, the Democratic Party, will have been out of power for twenty years of a twenty-four year span. Since 1968, numerous reforms in the presidential nominating process have been considered and adopted by the Democrats. These reforms have had the effect of opening the nominating process to rank-and-file Democrats through state primaries and participatory caucuses. While the reforms achieve this purpose, the end result is a mixed system that has been described as a disjointed hodgepodge of rules. Copyright 1989 by The Policy Studies Organization.