1,000 research outputs found
Isoproterenol and 8-bromo-cyclic adenosine monophosphate stimulate the expression of the angiotensinogen gene in opossum kidney cells
Isoproterenol and 8-bromo-cyclic adenosine monophosphate stimulate the expression of the angiotensinogen gene in opossum kidney cells. To investigate whether the expression of the renal angiotensinogen (ANG) gene is regulated by β-adrenoceptors and the cAMP-dependent protein kinase A pathway, we introduced stably the fusion gene containing the 5′-flanking regulatory sequence of the ANG gene with a human growth hormone (hGH) gene as a reporter, pOGH (ANG N-1498/+18), into opossum kidney (OK) cells. We successfully obtained several stable transformants with a high expression of the pOGH (ANG N-1498/+18) fusion gene. One stable transformant (OK 27) that is able to maintain the expression of pOGH (ANG N-1498/+18) in culture for more than a year was used in the present study. The level of expression of the pOGH (ANG N-1498/+18) in OK 27 was evaluated by the amount of immunoreactive-hGH (IR-hGH) secreted into the culture medium. The addition of isoproterenol (10-11 M to 10-9 M) stimulated the expression of pOGH (ANG N-1498/+18) and increased the accumulation of intracellular cAMP. Higher concentrations of isoproterenol (that is, greater than 10-9 M) had low or minimal effect. In contrast, the addition of 8-bromo-cAMP (8-Br-cAMP) and forskolin stimulated the expression of pOGH (ANG N-1498/+18) in a dose-dependent manner. The stimulatory effect of isoproterenol was blocked by the presence of propranolol, atenolol and ICI 118,551. The addition of ICI 118,551, however, was less effective than atenolol. Furthermore, the stimulatory effect of isoproterenol and 8-Br-cAMP on the expression of the pOGH (ANG N-1498/+18) was inhibited by the presence of Rp-cAMP (an inhibitor of cAMP-dependent protein kinase AI and II). These studies demonstrated that the expression of the pOGH (ANG N-1498/+18) in OK cells is stimulated by β-adrenoceptors and the cAMP-dependent protein kinase A pathway. Our data indicate that OK 27 cells provide a useful model to study the regulation of expression of the ANG gene in vitro
FastJet user manual
FastJet is a C++ package that provides a broad range of jet finding and
analysis tools. It includes efficient native implementations of all widely used
2-to-1 sequential recombination jet algorithms for pp and e+e- collisions, as
well as access to 3rd party jet algorithms through a plugin mechanism,
including all currently used cone algorithms. FastJet also provides means to
facilitate the manipulation of jet substructure, including some common boosted
heavy-object taggers, as well as tools for estimation of pileup and
underlying-event noise levels, determination of jet areas and subtraction or
suppression of noise in jets.Comment: 69 pages. FastJet 3 is available from http://fastjet.fr
Space-time inhomogeneity, anisotropy and gravitational collapse
We investigate the evolution of non-adiabatic collapse of a shear-free
spherically symmetric stellar configuration with anisotropic stresses
accompanied with radial heat flux. The collapse begins from a curvature
singularity with infinite mass and size on an inhomogeneous space-time
background. The collapse is found to proceed without formation of an even
horizon to singularity when the collapsing configuration radiates all its mass
energy. The impact of inhomogeneity on various parameters of the collapsing
stellar configuration is examined in some specific space-time backgrounds.Comment: To appear in Gen. Relativ. Gra
Thermodynamic and Tunneling Density of States of the Integer Quantum Hall Critical State
We examine the long wave length limit of the self-consistent Hartree-Fock
approximation irreducible static density-density response function by
evaluating the charge induced by an external charge. Our results are consistent
with the compressibility sum rule and inconsistent with earlier work that did
not account for consistency between the exchange-local-field and the disorder
potential. We conclude that the thermodynamic density of states is finite, in
spite of the vanishing tunneling density of states at the critical energy of
the integer quantum Hall transition.Comment: 5 pages, 4 figures, minor revisions, published versio
Cutaneous manifestations of hospitalized coronavirus disease 2019 patients: a report of six cases with clinicopathologic features and viral RNA in situ hybridization
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/163863/1/jdv16741.pdfhttp://deepblue.lib.umich.edu/bitstream/2027.42/163863/2/jdv16741_am.pd
New Supersymmetric String Compactifications
We describe a new class of supersymmetric string compactifications to 4d
Minkowski space. These solutions involve type II strings propagating on
(orientifolds of) non Calabi-Yau spaces in the presence of background NS and RR
fluxes. The simplest examples have descriptions as cosets, generalizing the
three-dimensional nilmanifold. They can also be thought of as twisted tori. We
derive a formula for the (super)potential governing the light fields, which is
generated by the fluxes and certain ``twists'' in the geometry. Detailed
consideration of an example also gives strong evidence that in some cases,
these exotic geometries are related by smooth transitions to standard
Calabi-Yau or G2 compactifications of M-theory.Comment: 43 pages, harvmac bi
Improved tests of extra-dimensional physics and thermal quantum field theory from new Casimir force measurements
We report new constraints on extra-dimensional models and other physics
beyond the Standard Model based on measurements of the Casimir force between
two dissimilar metals for separations in the range 0.2--1.2 m. The Casimir
force between an Au-coated sphere and a Cu-coated plate of a
microelectromechanical torsional oscillator was measured statically with an
absolute error of 0.3 pN. In addition, the Casimir pressure between two
parallel plates was determined dynamically with an absolute error of mPa. Within the limits of experimental and theoretical errors, the results
are in agreement with a theory that takes into account the finite conductivity
and roughness of the two metals. The level of agreement between experiment and
theory was then used to set limits on the predictions of extra-dimensional
physics and thermal quantum field theory. It is shown that two theoretical
approaches to the thermal Casimir force which predict effects linear in
temperture are ruled out by these experiments. Finally, constraints on Yukawa
corrections to Newton's law of gravity are strengthened by more than an order
of magnitude in the range 56 nm to 330 nm.Comment: Revtex 4, 35 pages, 14 figures in .gif format, accepted for
publication in Phys. Rev.
Recommended from our members
Bcl-2–Modifying Factor Induces Renal Proximal Tubular Cell Apoptosis in Diabetic Mice
This study investigated the mechanisms underlying tubular apoptosis in diabetes by identifying proapoptotic genes that are differentially upregulated by reactive oxygen species in renal proximal tubular cells (RPTCs) in models of diabetes. Total RNAs isolated from renal proximal tubules (RPTs) of 20-week-old heterozygous db/m+, db/db, and db/db catalase (CAT)-transgenic (Tg) mice were used for DNA chip microarray analysis. Real-time quantitative PCR assays, immunohistochemistry, and mice rendered diabetic with streptozotocin were used to validate the proapoptotic gene expression in RPTs. Cultured rat RPTCs were used to confirm the apoptotic activity and regulation of proapoptotic gene expression. Additionally, studies in kidney tissues from patients with and without diabetes were used to confirm enhanced proapoptotic gene expression in RPTs. Bcl-2–modifying factor (Bmf) was differentially upregulated (P < 0.01) in RPTs of db/db mice compared with db/m+ and db/db CAT-Tg mice and in RPTs of streptozotocin-induced diabetic mice in which insulin reversed this finding. In vitro, Bmf cDNA overexpression in rat RPTCs coimmunoprecipated with Bcl-2, enhanced caspase-3 activity, and promoted apoptosis. High glucose (25 mmol/L) induced Bmf mRNA expression in RPTCs, whereas rotenone, catalase, diphenylene iodinium, and apocynin decreased it. Knockdown of Bmf with small interfering RNA reduced high glucose–induced apoptosis in RPTCs. More important, enhanced Bmf expression was detected in RPTs of kidneys from patients with diabetes. These data demonstrate differential upregulation of Bmf in diabetic RPTs and suggest a potential role for Bmf in regulating RPTC apoptosis and tubular atrophy in diabetes
Gravitational collapse without a remnant
We investigate the gravitational collapse of a spherically symmetric,
inhomogeneous star, which is described by a perfect fluid with heat flow and
satisfies the equation of state or p=C\rho^\ga at its center.
Different from the ordinary process of gravitational collapsing, the energy of
the whole star is emitted into space. And the remaining spacetime is a
Minkowski one at the end of the process.Comment: 9 pages, 9 figures, to appear in Int. J. Theor. Phy
- …