Cellular IP₆ Levels Limit HIV Production while Viruses that Cannot Efficiently Package IP₆ Are Attenuated for Infection and Replication

Summary: HIV-1 hijacks host proteins to promote infection. Here we show that HIV is also dependent upon the host metabolite inositol hexakisphosphate (IP6) for viral production and primary cell replication. HIV-1 recruits IP6 into virions using two lysine rings in its immature hexamers. Mutation of either ring inhibits IP6 packaging and reduces viral production. Loss of IP6 also results in virions with highly unstable capsids, leading to a profound loss of reverse transcription and cell infection. Replacement of one ring with a hydrophobic isoleucine core restores viral production, but IP6 incorporation and infection remain impaired, consistent with an independent role for IP6 in stable capsid assembly. Genetic knockout of biosynthetic kinases IPMK and IPPK reveals that cellular IP6 availability limits the production of diverse lentiviruses, but in the absence of IP6, HIV-1 packages IP5 without loss of infectivity. Together, these data suggest that IP6 is a critical cofactor for HIV-1 replication

Current correlators to all orders in the quark masses

The contributions to the coefficient functions of the quark and the mixed quark-gluon condensate to mesonic correlators are calculated for the first time to all orders in the quark masses, and to lowest order in the strong coupling constant. Existing results on the coefficient functions of the unit operator and the gluon condensate are reviewed. The proper factorization of short- and long-distance contributions in the operator product expansion is discussed in detail. It is found that to accomplish this task rigorously the operator product expansion has to be performed in terms of non-normal-ordered condensates. The resulting coefficient functions are improved with the help of the renormalization group. The scale invariant combination of dimension 5 operators, including mixing with the mass operator, which is needed for the renormalization group improvement, is calculated in the leading order.Comment: 24 pages, LateX file, TUM-T31-21/92, 1 postscript file include

Gauge Theory of Composite Fermions: Particle-Flux Separation in Quantum Hall Systems

Fractionalization phenomenon of electrons in quantum Hall states is studied in terms of U(1) gauge theory. We focus on the Chern-Simons(CS) fermion description of the quantum Hall effect(QHE) at the filling factor $\nu=p/(2pq\pm 1)$, and show that the successful composite-fermions(CF) theory of Jain acquires a solid theoretical basis, which we call particle-flux separation(PFS). PFS can be studied efficiently by a gauge theory and characterized as a deconfinement phenomenon in the corresponding gauge dynamics. The PFS takes place at low temperatures, $T \leq T_{\rm PFS}$, where each electron or CS fermion splinters off into two quasiparticles, a fermionic chargeon and a bosonic fluxon. The chargeon is nothing but Jain's CF, and the fluxon carries $2q$ units of CS fluxes. At sufficiently low temperatures $T \leq T_{\rm BC} (< T_{\rm PFS})$, fluxons Bose-condense uniformly and (partly) cancel the external magnetic field, producing the correlation holes. This partial cancellation validates the mean-field theory in Jain's CF approach. FQHE takes place at $T < T_{\rm BC}$ as a joint effect of (i) integer QHE of chargeons under the residual field $\Delta B$ and (ii) Bose condensation of fluxons. We calculate the phase-transition temperature $T_{\rm PFS}$ and the CF mass. PFS is a counterpart of the charge-spin separation in the t-J model of high-$T_{\rm c}$ cuprates in which each electron dissociates into holon and spinon. Quasiexcitations and resistivity in the PFS state are also studied. The resistivity is just the sum of contributions of chargeons and fluxons, and $\rho_{xx}$ changes its behavior at $T = T_{\rm PFS}$, reflecting the change of quasiparticles from chargeons and fluxons at $T < T_{\rm PFS}$ to electrons at $T_{\rm PFS} < T$.Comment: 18 pages, 7 figure

Beyond locutionary denotations: exploring trust between practitioners and policy

This study reports the findings of a research on the trust relationship between practitioners in the Skills for Life (SfL) area and the policy that informs their practice. The exploration of this relationship was premised on an extended notion of trust relationship which draws from the Speech Act theory of Austin (1962; Searle 1969; Kissine 2008), leading to the claim that the existence of different layers of imports in textual analysis makes it possible for a trust relationship to exist between the human/physical and the non human/non physical. The study found that the majority of practitioners in the SfL field trust policy to deliver its inherent policy only to a limited extent. Amongst others, the study identified the impact of the perlocutionary import of policy text on practitioners as a viable reason for this limited level of trust. Such perlocutionary imports, it also found, have adverse impact on practitioners who are considered to have drawn from previous experience to mediate the import of contemporary policies

ADM formulation of the General Relativity

Se realiza el estudio del formalismo ADM para la Teoría de la Relatividad General mediante el método de las foliaciones en las superficies de Cauchy. Se presenta explícitamente el desarrollo matemático que conduce, finalmente, a la formulación hamiltoniana de la Relatividad General.The study of the ADM formalism for the General Relativity Theory through the foliation methods on the Cauchy surfacesis is realized. The mathematical development that leads to the Hamiltonian formulation of General Relativity is presented explicitly

H2 in the interstitial channels of nanotube bundles

The equation of state of H2 adsorbed in the interstitial channels of a carbon nanotube bundle has been calculated using the diffusion Monte Carlo method. The possibility of a lattice dilation, induced by H2 adsorption, has been analyzed by modeling the cohesion energy of the bundle. The influence of factors like the interatomic potentials, the nanotube radius and the geometry of the channel on the bundle swelling is systematically analyzed. The most critical input is proved to be the C-H2 potential. Using the same model than in planar graphite, which is expected to be also accurate in nanotubes, the dilation is observed to be smaller than in previous estimations or even inexistent. H2 is highly unidimensional near the equilibrium density, the radial degree of freedom appearing progressively at higher densities.Comment: Accepted for publication in PR

SS Ari: a shallow-contact close binary system

Two CCD epochs of light minimum and a complete R light curve of SS Ari are presented. The light curve obtained in 2007 was analyzed with the 2003 version of the W-D code. It is shown that SS Ari is a shallow contact binary system with a mass ratio $q=3.25$ and a degree of contact factor f=9.4(\pm0.8%). A period investigation based on all available data shows that there may exist two distinct solutions about the assumed third body. One, assuming eccentric orbit of the third body and constant orbital period of the eclipsing pair results in a massive third body with $M_3=1.73M_{\odot}$ and P_3=87.0$yr. On the contrary, assuming continuous period changes of the eclipsing pair the orbital period of tertiary is 37.75yr and its mass is about$0.278M_{\odot}$. Both of the cases suggest the presence of an unseen third component in the system.Comment: 28 pages, 9 figures and 5 table

Neutron scattering and molecular correlations in a supercooled liquid

We show that the intermediate scattering function $S_n(q,t)$ for neutron scattering (ns) can be expanded naturely with respect to a set of molecular correlation functions that give a complete description of the translational and orientational two-point correlations in the liquid. The general properties of this expansion are discussed with special focus on the $q$-dependence and hints for a (partial) determination of the molecular correlation functions from neutron scattering results are given. The resulting representation of the static structure factor $S_n(q)$ is studied in detail for a model system using data from a molecular dynamics simulation of a supercooled liquid of rigid diatomic molecules. The comparison between the exact result for $S_n(q)$ and different approximations that result from a truncation of the series representation demonstrates its good convergence for the given model system. On the other hand it shows explicitly that the coupling between translational (TDOF) and orientational degrees of freedom (ODOF) of each molecule and rotational motion of different molecules can not be neglected in the supercooled regime.Further we report the existence of a prepeak in the ns-static structure factor of the examined fragile glassformer, demonstrating that prepeaks can occur even in the most simple molecular liquids. Besides examining the dependence of the prepeak on the scattering length and the temperature we use the expansion of $S_n(q)$ into molecular correlation functions to point out intermediate range orientational order as its principle origin.Comment: 13 pages, 7 figure

Post-subduction tectonics induced by extension from a lithospheric drip

Acknowledgements S.P. acknowledges support from the Natural Environmental Research Council (NERC) Grant NE/R013500/1 and from the European Union’s Horizon 2020 Research and Innovation Program under Marie Skłodowska-Curie Grant Agreement 790203. We thank the TanDEM-X Science Communication Team (German Aerospace Center (DLR) e.V.) for providing TanDEM topographic data. We thank the NERC Geophysical Equipment Facility for loan 1038. Numerical simulations were undertaken on the NCI National Facility in Canberra, Australia, which is supported by the Australian Commonwealth Government. A.G. was funded by an Independent Research Fellowship from the Royal Astronomical Society.Peer reviewedPostprin

Kinetics of plasma cell‐free DNA and creatine kinase in a canine model of tissue injury

Background: Cell‐free DNA (cfDNA) comprises short, double‐stranded circulating DNA sequences released from damaged cells. In people, cfDNA concentrations correlate well with disease severity and tissue damage. No reports are available regarding cfDNA kinetics in dogs. Objectives/Hypothesis: Cell‐free DNA will have a short biological half‐life and would be able to stratify mild, moderate, and severe tissue injury. Our study aims were to determine the kinetics and biological half‐life of cfDNA and to contrast them with those of creatine kinase (CK). Animals: Three groups of 10 dogs undergoing open ovariohysterectomy, surgery for cranial cruciate ligament rupture (CCLR), or hemilaminectomy. Methods: Plasma for cfDNA and CK analysis was collected at admission, at induction of anesthesia, postsurgery (time 0) and at 6, 12, 24, 36, 48, 60, and 72 hours after surgery. Results: The biological half‐life of plasma cfDNA and CK were 5.64 hours (95% confidence interval [CI 95], 4.36–7.98 hours) and 28.7 hours (CI95, 25.3–33.3 hours), respectively. In the hemilaminectomy group, cfDNA concentrations differed significantly from admission at 6–12 hours after surgery. Creatine kinase activity differed among the surgical groups and reached a peak 6 hours after surgery. In the ovariohysterectomy and CCLR groups, plasma CK activity 72 hours after surgery did not differ from admission activity of the ovariohysterectomy group. In contrast, in the hemilaminectomy group, plasma CK activity after 72 hours did not return to the ovariohysterectomy group admission activity. Conclusions and Clinical Importance: Plasma CK activity has a longer biological half‐life than previously thought. In contrast to plasma CK activity, cfDNA has a short half‐life and could be a useful marker for peracute severe tissue injury