396 research outputs found
Kinetics of acute hepatitis B virus infection in humans
Using patient data from a unique single source outbreak of hepatitis B virus (HBV) infection, we have characterized the kinetics of acute HBV infection by monitoring viral turnover in the serum during the late incubation and clinical phases of the disease in humans. HBV replicates rapidly with minimally estimated doubling times ranging between 2.2 and 5.8 d (mean 3.7 ± 1.5 d). After a peak viral load in serum of nearly 1010 HBV DNA copies/ml is attained, clearance of HBV DNA follows a two or three phase decay pattern with an initial rapid decline characterized by mean half-life (t1/2) of 3.7 ± 1.2 d, similar to the t1/2 observed in the noncytolytic clearance of covalently closed circular DNA for other hepadnaviruses. The final phase of virion clearance occurs at a variable rate (t1/2 of 4.8 to 284 d) and may relate to the rate of loss of infected hepatocytes. Free virus has a mean t1/2 of at most 1.2 ± 0.6 d. We estimate a peak HBV production rate of at least 1013 virions/day and a maximum production rate of an infected hepatocyte of 200–1,000 virions/day, on average. At this peak rate of virion production we estimate that every possible single and most double mutations would be created each day
Demonstration of a Transportable 1 Hz-Linewidth Laser
We present the setup and test of a transportable clock laser at 698 nm for a
strontium lattice clock. A master-slave diode laser system is stabilized to a
rigidly mounted optical reference cavity. The setup was transported by truck
over 400 km from Braunschweig to D\"usseldorf, where the cavity-stabilized
laser was compared to a stationary clock laser for the interrogation of
ytterbium (578 nm). Only minor realignments were necessary after the transport.
The lasers were compared by a Ti:Sapphire frequency comb used as a transfer
oscillator. The thus generated virtual beat showed a combined linewidth below 1
Hz (at 1156 nm). The transport back to Braunschweig did not degrade the laser
performance, as was shown by interrogating the strontium clock transition.Comment: 3 pages, 4 figure
Optical fibers with interferometric path length stability by controlled heating for transmission of optical signals and as components in frequency standards
We present a simple method to stabilize the optical path length of an optical
fiber to an accuracy of about 1/100 of the laser wavelength. We study the
dynamic response of the path length to modulation of an electrically conductive
heater layer of the fiber. The path length is measured against the laser
wavelength by use of the Pound-Drever-Hall method; negative feedback is applied
via the heater. We apply the method in the context of a cryogenic resonator
frequency standard.Comment: Expanded introduction and outlook. 9 pages, 5 figure
Optogalvanic Spectroscopy of Metastable States in Yb^{+}
The metastable ^{2}F_{7/2} and ^{2}D_{3/2} states of Yb^{+} are of interest
for applications in metrology and quantum information and also act as dark
states in laser cooling. These metastable states are commonly repumped to the
ground state via the 638.6 nm ^{2}F_{7/2} -- ^{1}D[5/2]_{5/2} and 935.2 nm
^{2}D_{3/2} -- ^{3}D[3/2]_{1/2} transitions. We have performed optogalvanic
spectroscopy of these transitions in Yb^{+} ions generated in a discharge. We
measure the pressure broadening coefficient for the 638.6 nm transition to be
70 \pm 10 MHz mbar^{-1}. We place an upper bound of 375 MHz/nucleon on the
638.6 nm isotope splitting and show that our observations are consistent with
theory for the hyperfine splitting. Our measurements of the 935.2 nm transition
extend those made by Sugiyama et al, showing well-resolved isotope and
hyperfine splitting. We obtain high signal to noise, sufficient for laser
stabilisation applications.Comment: 8 pages, 5 figure
Nitrogen Retention in Headwater Streams: The Influence of Groundwater-Surface Water Exchange
Groundwater-surface water (GW-SW) interaction lengthens hydraulic residence times, increases contact between solutes and biologically active surfaces, and often creates a gradient of redox conditions conducive to an array of biogeochemical processes. As such, the interaction of hydraulic patterns and biogeochemical activity is suspected to be an important determinant of elemental spiraling in streams. Hydrologic interactions may be particularly important in headwater streams, where the extent of the GW-SW mixing environment (i.e., hyporheic zone) is proportionately greater than in larger streams. From our current understanding of stream ecosystem function, we discuss nitrogen (N) spiraling, present a conceptual model of N retention in streams, and use both of these issues to generate specific research questions and testable hypotheses regarding N dynamics in streams
Deterministic delivery of externally cold and precisely positioned single molecular ions
We present the preparation and deterministic delivery of a selectable number
of externally cold molecular ions. A laser cooled ensemble of Mg^+ ions
subsequently confined in several linear Paul traps inter-connected via a
quadrupole guide serves as a cold bath for a single or up to a few hundred
molecular ions. Sympathetic cooling embeds the molecular ions in the
crystalline structure. MgH^+ ions, that serve as a model system for a large
variety of other possible molecular ions, are cooled down close to the Doppler
limit and are positioned with an accuracy of one micrometer. After the
production process, severely compromising the vacuum conditions, the molecular
ion is efficiently transfered into nearly background-free environment. The
transfer of a molecular ion between different traps as well as the control of
the molecular ions in the traps is demonstrated. Schemes, optimized for the
transfer of a specific number of ions, are realized and their efficiencies are
evaluated. This versatile source applicable for broad charge-to-mass ratios of
externally cold and precisely positioned molecular ions can serve as a
container-free target preparation device well suited for diffraction or
spectroscopic measurements on individual molecular ions at high repetition
rates (kHz).Comment: 11 pages, 8 figure
Amplitude to phase conversion of InGaAs pin photo-diodes for femtosecond lasers microwave signal generation
When a photo-diode is illuminated by a pulse train from a femtosecond laser,
it generates microwaves components at the harmonics of the repetition rate
within its bandwidth. The phase of these components (relative to the optical
pulse train) is known to be dependent on the optical energy per pulse. We
present an experimental study of this dependence in InGaAs pin photo-diodes
illuminated with ultra-short pulses generated by an Erbium-doped fiber based
femtosecond laser. The energy to phase dependence is measured over a large
range of impinging pulse energies near and above saturation for two typical
detectors, commonly used in optical frequency metrology with femtosecond laser
based optical frequency combs. When scanning the optical pulse energy, the
coefficient which relates phase variations to energy variations is found to
alternate between positive and negative values, with many (for high harmonics
of the repetition rate) vanishing points. By operating the system near one of
these vanishing points, the typical amplitude noise level of commercial-core
fiber-based femtosecond lasers is sufficiently low to generate state-of-the-art
ultra-low phase noise microwave signals, virtually immune to amplitude to phase
conversion related noise.Comment: 7 pages, 6 figures, submitted to Applied Physics
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