Effect of daily movement of dairy cattle to fresh grass in morning or afternoon on intake, grazing behaviour, rumen fermentation and milk production

Twenty Holstein cows were split into two equal groups to test the effect of daily move to a previously ungrazed strip after morning milking (MA) or afternoon milking (AA) on herbage intake, grazing behaviour, rumen characteristics and milk production using a randomized block design with three periods of 14 days each. Milking took place at 06.00 and 16.00 h. The chemical composition of grass was similar between treatments, but an interaction between treatment and time of sampling was found in all variables except acid detergent lignin (ADL). The most pronounced differences existed in sugar content. Grass sugar content was greatest following afternoon milking. However, the difference in sugar content in grass was much larger in MA (158 v 114 g/kg dry matter (DM) at 16.00 and 06.00 h, respectively) than in AA (147 v 129 g/kg DM at 16.00 and 06.00 h, respectively). Neutral detergent fibre (NDF) was significantly higher at 06.00 h than at 16.00 h (469 v 425 g/kg DM) in AA, but was equal between morning and afternoon in MA (453 g/kg DM). Herbage intake, determined using the n-alkane technique, did not differ between treatments. Grazing behaviour observed using IGER graze recorders were similar between treatments, except for ruminating time, bite rate and the number of ruminations and boli per period of the day. However, interactions between treatment and time in grazing behaviour variables were found. Grazing time was longer and number of bites was greater following allocation to a new plot (after milking in the morning in MA or milking in the afternoon in AA) when compared to allocation to the same plot after the subsequent milking per treatment (after milking in the afternoon or morning in MA and AA, respectively). In comparison to AA, grazing time in MA was more evenly distributed during the day but lower during the night. The combined effects of differences in grazing behaviour and chemical composition of the grass between treatments in different periods of the day probably caused higher intake of sugars in AA, resulting in a significantly higher non-glucogenic to glucogenic volatile fatty acid ratio (NGR) in the rumen in AA than MA. Milk fat content was lower in MA than AA, but milk production and milk protein and lactose content did not differ. In conclusion, time of allocation to a fresh plot altered the distribution of grazing behaviour variables over the day, and affected NGR and milk fat content, but herbage intake and milk production were not change

The influence of the method of preservation of forages on the digestion in dairy cows. 2. Digestion of organic matter, energy and amino acids in forestomachs and intestines.

2. Two dairy cows with duodenal reentrant and rumen cannulae were given diets containing artificially dried and pelleted grass (GP), grass silage treated with formic acid (GSF), grass silage treated with a mixture of formic acid and formaldehyde (GSFF) or grass silage without additive (GS). DM intake was 14.8 to 16.0 kg/day and about 30% of the N in the diets was from grass pellets or silages. Between 45 and 57% of the apparently digested organic matter and between 26 and 41% of the apparently digested energy disappeared before the intestines. The higher values were found with GSF and GS.Total amino acid N reaching the duodenum was between 104 and 134% of intake, highest with GP and GSFF. The amounts of individual amino acids reaching the small intestine were from 75 to 270% of the amounts ingested. High values (>150%) were found for glycine, lysine, methionine and tyrosine; low values

Effect of the roughage/concentrate ratio on nitrogen entering the small intestine of dairy cows.

The effect of different roughage:concentrate ratios on N entering the small intestine was studied in groups of 2 to 5 cows with a rumen fistula and re-entrant cannulae at the beginning of the small intestine. In 3 series there were 10 treatments in which DM intake ranged between 3.8 and 15.7 kg daily; the proportion of long roughage in the diet was from 29 to 81%. The apparent digestibility of organic matter (O), crude fibre (XF) and nitrogen-free extracts (XX) was 76, 69 and 81%, respectively. The proportion of the digestion taking place in the stomach was 59, 94 and 76%. A larger proportion of the apparently digestible organic matter tended to be digested in the stomach when there was a larger proportion of long roughage in the diet. The contribution of microbial N to the intestinal N was estimated. From this the efficiency of microbial protein synthesis was estimated and related to the amount of carbohydrates (XF + XX) fermented in the stomach. Per kg carbohydrates fermented, 32 plus or minus 1.5 g microbial N was produced. The percentage of dietary N not degraded in the stomach averaged 30 plus or minus 1.5. Varying the ratio between long roughage and pelleted concentrates seemed to have little effect on the degradation of dietary protein or on the efficiency of microbial protein synthesis. With long roughage in the diet between 29 and 81% there seems little effect of varying the roughage:concentrate ratio on the protein supply in dairy cows. (Abstract retrieved from CAB Abstracts by CABI’s permission

Start-up inertia as an origin for heterogeneous flow

For quite some time non-monotonic flow curve was thought to be a requirement for shear banded flows in complex fluids. Thus, in simple yield stress fluids shear banding was considered to be absent. Recent spatially resolved rheological experiments have found simple yield stress fluids to exhibit shear banded flow profiles. One proposed mechanism for the initiation of such transient shear banding process has been a small stress heterogeneity rising from the experimental device geometry. Here, using Computational Fluid Dynamics methods, we show that transient shear banding can be initialized even under homogeneous stress conditions by the fluid start-up inertia, and that such mechanism indeed is present in realistic experimental conditions

Duration of Regrowth of Ryegrass (\u3ci\u3eLolium perenne\u3c/i\u3e) Swards: Effects on Rumen Fermentation of Lactating Dairy Cows

The relative importance of duration of sward regrowth and rumen fill and fermentation on the control of grazing time and intake rate during the first grazing session of the day were studied. Four lactating dairy cows were allowed to graze ryegrass (Lolium perenne) swards, with five different regrowth periods after mowing (6, 9, 16, 22 and 30 d). The cows were allowed to graze until they stopped voluntarily. Samples of rumen liquid were taken at approximately 0, 30, 60, 120 and 240 min after the grazing session was finished. Concentration of volatile fatty acids (VFA) followed a significant quadratic trend with a maximum concentration observed at approximately 110 min after cessation of grazing. In this study, rumen fill, VFA (either total or major components) and ammonia concentration as individual variables were not correlated with grazing time or dry matter intake

EFFECT OF FEED PROCESSING ON VOLATILE FATTY ACID PRODUCTION RATES MEASURED WITH 13C-ACETATE IN GRAZING LACTATING DAIRY COWS

The effect of processed cereal grain supplementation on volatile fatty acid (VFA) production rates of grazing, lactating Holstein-Friesian cows were measured in a 5x5 Latin square experiment. The experimental treatments were as follows: control (only grazing, no supplement addition, NS), pelleted barley (PB), pelleted maize (PM), toasted and subsequently pelleted barley (TPB), and toasted and subsequently pelleted maize (TPM) as supplements. An isotope dilution technique using stable isotope of carbon (13C) as an internal marker was employed for the estimation of VFA production. At the beginning of a 3-hour long allowed grazing time, 100 mg of 99% enriched 13C2 Na-acetate were introduced in the rumen and repeated after grazing with 50 mg isotope, after which the cows were starved for 6 hours until evening milking. During grazing disappearance rate (kdis) and production rate (kprod) of acetate, propionate and butyrate were significantly higher (P≤0.05) in supplemented than in NS cows. Moreover the effect of barley grain and pelleting treatment was higher than the effect of maize grain and toasting. During starvation significantly higher (P≤0.05) kdis and kprod of VFAs were observed in PM and TPM treatments. Total VFA production for the experimental period (grazing + starvation) were 49.5, 78.7, 69.9, 88.5, 80.8 mol/day for NS, PB, TPB, PM and TPM, respectively. The higher VFA productions measured in supplemented animals emphasis the extensive digestion that occurs in the rumen after feeding processed grains. In methodological terms, 13C2 Na-acetate labelling appears to be a useful means for examining the VFA acetate production in ruminants

The influence of the method of preservation of forages on the digestion in dairy cows. 1. Composition of the forages and digestibility of dry matter, organic matter and nitrogen.

1. Grass from the same sward was ensiled without additive, with 14.6 g formic acid/100 g crude protein or 10.8 g formic acid and 10.6 g formaldehyde/100 g crude protein. Similar grass was dried and pelleted. Drying or ensiling with the mixture reduced solubility of N in the preserved grass but formic acid increased it, and ensiling without additive increased it even more. Apparent digestibility of N in the rumen of cows tended to decrease with decrease in solubility. Digestibility in vitro of the mixed diet given to the cows, calculated from digestibility of the separate components, agreed well with the values in vivo for diets with silages, but was high for that with dried grass. (Abstract retrieved from CAB Abstracts by CABI’s permission