Effect of adjuvants and nitrogen sources on in vitro shoot regeneration and clonal propagation of medicinally important plant Eryngium foetidum L

In vitro regeneration of medicinally important plant Eryngium foetidum L. was established by developing a protocol for successful faster clonal propogation. Young leaves of the plant were taken and cultured on 1x MS medium supplemented with different concentrations and combinations of 6- Benzyl amino purine (BAP), 1-Napthylacetic acid (NAA) and Kinetin (Kin). Leaves cultured on 2mg/L BAP and 1mg/L NAA showed better response in short duration of time when compared to other concentrations and combinations. Induced shoots obtained from standardized media were used as explant and cultured on 1x MS medium containing 2mg/L BAP and 1mg/L NAA fortified with 10% of different nitrogen sources like  tryptone, peptone, beef extract, yeast extract and adjuvants like coconut milk, tomato juice and banana pulp to observe the growth response. It was observed that media containing 10% coconut milk and 10% peptone showed better result when compared to other adjuvants and nitrogen sources. The well developed plantlets were hardened and successfully transferred to the field with 85% survival rate

A comparative kinetic study on β-amylase and its antioxidant property in germinated and non germinated seeds of Glycine max. L

Enzyme activity plays a key role in each stage of plant development starting from the initial seed germination responses. A comparative analysis of beta amylase activity was performed, isolated from germinated and non germinated Glycine max seeds, which was partially purified by dialysis. The optimum pH and temperature for germinated seeds was found to be 4.5 and 370 C and that for non germinated seeds were 5.5 and 750 C respectively. MnCl2 and CoCl2 exhibited marked activating effect on the enzyme, while HgCl2 was a potent inhibitor for both seeds. The Km and Vmax value for β-amylase with soluble starch as substrate was found to be 3.03mg/ml and 6.6micromol/min/ml for germinated seed and for non germinated seeds it was found to be 5mg/m and 10micromole/min/ml respectively. The molecular weight of partially purified enzyme was 57±1kDa on SDS PAGE with 1.918 fold purification and 27.98% yield for germinated seeds and for non germinated seeds the purification fold was 1.507 with 26.65% yield. The enzyme showed highest amount of total antioxidant activity of 304µg/ml in partially purified germinated seed. For ABTS free radical scavenging activity, partially purified enzyme from germinated seed showed the highest of 76.09% activity with an IC50 value of 58.20µg/ml. Germinated seeds have highest enzymatic activity which can be considered for several industrial purposes

Analysis of airborne Doppler lidar, Doppler radar and tall tower measurements of atmospheric flows in quiescent and stormy weather

The first experiment to combine airborne Doppler Lidar and ground-based dual Doppler Radar measurements of wind to detail the lower tropospheric flows in quiescent and stormy weather was conducted in central Oklahoma during four days in June-July 1981. Data from these unique remote sensing instruments, coupled with data from conventional in-situ facilities, i.e., 500-m meteorological tower, rawinsonde, and surface based sensors, were analyzed to enhance understanding of wind, waves and turbulence. The purposes of the study were to: (1) compare winds mapped by ground-based dual Doppler radars, airborne Doppler lidar, and anemometers on a tower; (2) compare measured atmospheric boundary layer flow with flows predicted by theoretical models; (3) investigate the kinematic structure of air mass boundaries that precede the development of severe storms; and (4) study the kinematic structure of thunderstorm phenomena (downdrafts, gust fronts, etc.) that produce wind shear and turbulence hazardous to aircraft operations. The report consists of three parts: Part 1, Intercomparison of Wind Data from Airborne Lidar, Ground-Based Radars and Instrumented 444 m Tower; Part 2, The Structure of the Convective Atmospheric Boundary Layer as Revealed by Lidar and Doppler Radars; and Part 3, Doppler Lidar Observations in Thunderstorm Environments

A Brokering Framework for Assessing Legal Risks in Big Data and the Cloud

“Cloud computing” and “Big Data” are amongst the most hyped-up terms and buzzwords of the moment. After decades in which individuals and companies used to host their data and applications using their own IT infrastructure, the world has seen the stunning transformation of the Internet. Major shifts occurred when these infrastructures began to be outsourced to public Cloud providers to match commercial expectations. Storing, sharing and transferring data and databases over the Internet is convenient, yet legal risks cannot be eliminated. Legal risk is a fast-growing area of research and covers various aspects of law. Current studies and research on Cloud computing legal risk assessment have been, however, limited in scope and focused mainly on security and privacy aspects. There is little systematic research on the risks, threats and impact of the legal issues inherent to database rights and “ownership” rights of data. Database rights seem to be outdated and there is a significant gap in the scientific literature when it comes to the understanding of how to apply its provisions in the Big Data era. This means that we need a whole new framework for understanding, protecting and sharing data in the Cloud. The scheme we propose in this chapter is based on a risk assessment-brokering framework that works side by side with Service Level Agreements (SLAs). This proposed framework will provide better control for Cloud users and will go a long way to increase confidence and reinforce trust in Cloud computing transactions