1,058 research outputs found
Opinion Exploiting genomics to improve the benefits of wheat: Prospects and limitations
Conventional breeding has been immensely successful in increasing crop production to meet the demands of the growing global population, particularly for wheat where production has increased by over threefold over the last 60 years without a significant increase in the area of land used. However, the pace of improvement by conventional breeding is slow and limited by the range of variation present in wheat and species with which it can be crossed. Genomics can be defined as “an interdisciplinary field of biology focusing on the structure, function, evolution, mapping, and editing of genomes” (Wikipedia). As such it has the potential to revolutionise crop improvement, by accelerating the rate of progress and increasing the range of variation that is available. Despite this potential, progress in the application of biotechnology to improve wheat has been slow, particularly when applied to the quality of the grain for processing and nutrition. We will therefore consider the reasons for this and identify priorities for future research
Hordein polypeptide pattern in relation to malting quality and the varietal identification of malted barley-grain
Partial-purification and preliminary characterization of soluble leaf proteins specific to virus-infected tobacco plants
Substantial increase in yield predicted by wheat ideotypes for Europe under future climate
A substantial increase in food production is needed for global food security. Europe is the largest wheat producer, delivering 35% of wheat globally, but its future genetic yield potential is yet unknown. We estimated the genetic yield potential of wheat in Europe under 2050 climate by designing in silico wheat ideotypes based on genetic variation in wheat germplasm. To evaluate the importance of heat and drought stresses around flowering, a critical stage in wheat development, sensitive and tolerant ideotypes were designed. Ideotype yields ranged from 9 to 17 t ha−1 across major wheat growing regions in Europe under 2050 climate. Both ideotypes showed a substantial increase in yield of 66−89% compared to current local cultivars under future climate. Key traits for wheat improvements under future climate were identified. Ideotype design is a powerful tool for estimating crop genetic yield potential in a target environment, along with the potential to accelerate breeding by providing target traits for improvements
What do we really understand about wheat gluten structure and functionality?
The structure and functional properties of wheat gluten have fascinated cereal chemists for over a century and a range of approaches have been taken to understand the structures and interactions of the gluten protein complex and how these are established. Nevertheless, our knowledge is still far from complete. We therefore review the current state of our knowledge and identify gaps and priorities for future research. The evidence for the forces that determine the interactions of the individual proteins in the gluten complex is re-evaluated, which allows us to define the relative contributions of covalent disulphide bonds and non-covalent forces (hydrogen bonds, hydrophobic and electrostatic interactions) and to relate these interactions to the amino acid sequences, structures and properties of the individual protein subunits. We also discuss the evidence for the pathway of gluten protein synthesis, deposition and assembly in the developing grain and how the assembly may be modified during the maturation of the grain
A stealth health approach to dietary fibre
Average dietary fibre intakes have increased little in the past twenty years in many countries, including the USA1. Multi-million-dollar campaigns promoting fruits, vegetables, whole grains and other foods high in fibre have delivered only small changes in diets2, and consumers have not changed from traditional staples to whole-grain options3. UK millers report that consumption of whole-wheat bread has actually declined over the past decade (P. Shewry, personal communication). In the US, white flour, which is lower in fibre than whole-wheat flour, accounts for nearly 40% of the fibre intake4. We believe that as motivating consumers to change food choices has proven difficult, changing food itself — a so-called stealth health approach — could be a useful strategy to increase fibre in the foods people choose to eat
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Trafficking of storage proteins in developing grain of wheat
The processing properties of the wheat flour are largely determined by the structures and interactions of the grain storage proteins (also called gluten proteins) which form a continuous visco-elastic network in dough. Wheat gluten proteins are classically divided into two groups, the monomeric gliadins and the polymeric glutenins, with the latter being further classified into low molecular weight (LMW) and high molecular weight (HMW) subunits. The synthesis, folding and deposition of the gluten proteins take place within the endomembrane system of the plant cell. However, determination of the precise routes of trafficking and deposition of individual gluten proteins in developing wheat grain has been limited in the past by the difficulty of developing monospecific antibodies. To overcome this limitation, a single gluten protein (a LMW subunit) was expressed in transgenic wheat with a C-terminal epitope tag, allowing the protein to be located in the cells of the developing grain using highly specific antibodies. This approach was also combined with the use of wider specificity antibodies to compare the trafficking and deposition of different gluten protein groups within the same endosperm cells. These studies are in agreement with previous suggestions that two trafficking pathways occur in wheat, with the proteins either being transported via the Golgi apparatus into the vacuole or accumulating directly within the lumen of the ER. They also suggest that the same individual protein could be trafficked by either pathway, possibly depending on the stage of development, and that segregation of gluten proteins both between and within protein bodies may occur
A novel approach to identify genes that determine grain protein deviation in cereals
Grain yield and protein content were determined for six wheat cultivars grown over 3years at multiple sites and at multiple nitrogen (N) fertilizer inputs. Although grain protein content was negatively correlated with yield, some grain samples had higher protein contents than expected based on their yields, a trait referred to as grain protein deviation (GPD). We used novel statistical approaches to identify gene transcripts significantly related to GPD across environments. The yield and protein content were initially adjusted for nitrogen fertilizer inputs and then adjusted for yield (to remove the negative correlation with protein content), resulting in a parameter termed corrected GPD. Significant genetic variation in corrected GPD was observed for six cultivars grown over a range of environmental conditions (a total of 584 samples). Gene transcript profiles were determined in a subset of 161 samples of developing grain to identify transcripts contributing to GPD. Principal component analysis (PCA), analysis of variance (ANOVA) and means of scores regression (MSR) were used to identify individual principal components (PCs) correlating with GPD alone. Scores of the selected PCs, which were significantly related to GPD and protein content but not to the yield and significantly affected by cultivar, were identified as reflecting a multivariate pattern of gene expression related to genetic variation in GPD. Transcripts with consistent variation along the selected PCs were identified by an approach hereby called one-block means of scores regression (one-block MSR)
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