Analisis Marka Morfologi Dan Molekuler Sifat Ketahanan Kedelai Terhadap Intensitas Cahaya Rendah

Availability of moleculer marker to identify the important agronomic character of plant is needed to accelerate selection activity of plant. Particularly for the complex quantitatively inherited traits - like e.g. shading tolerance of soybean - the use of such a technique will speed up the process to produce adapted genotypes.The objective of this research is to identify the linkage of molecular marker RAPD with character of shading tolerance. The morphological specific characters wich is corelated to shade tolerance is the number of productive branchs. The intensity 75% of artificial shading is optimal level for doing selection of soybean genotypes. The inheritance of shading tolerance of soybean was controlled by gene with full dominant or by two genes pairs with dominant and ressesive epistasis. There was no maternal effect in the inheritance to shade tolerance. Heritability value (0.45 – 0.54) indicated that the proportion variation caused by the genetic factors was moderate. Molecular analysis by using RAPD technique showed that UBC153, ROTH 480.01, and ROTH 480.03 primer have polymorphic band that can be used for inheritance study and linkage analysis. All polymorphisms segregated independently of each ather. Interval mapping with Mapmaker/QTL indicated that the location of the three QTLs on linkage group were at marker tolerance locus of Roth 480.01-8125 , Roth 480.03-1125, and UBC 153-19125

Induksi Mutasi pada Stek Pucuk Anyelir (Dianthus Caryophyllus Linn.) melalui Iradiasi Sinar Gamma

It has been a common knowledge that LD50 is commonly used in estimating optimal doses of gamma irradiation in a breeding program. This research was aimed at observing radiosensitivity of five carnation's genotypes to gamma irradiation, to find the LD50 of carnation's cuttings, and to obtain solid mutants from five numbers of Carnation. For cuttings, carnation genotype number 10.8 was the most insensitive to gamma rays, whereas number 24.15 was the most sensitive one. LD50 of carnation's cuttings were obtained around 49 -72 gray. There were 19 mutants produced from this treatment. The desired mutans were mostly produced from the treated 24.1 genotype whereas the character mutans were mostly observed in MV2 generation. Irradiation treatment on genotype 24.1 produced most stabile mutans while the less was in genotype 24.14. The produced mutants were qualitatively different in colour and petal shape, and stabile till third generation

KORELASI GENOTIPIK ANTARA HASIL DENGAN TINGKAT KETAHANAN TERHADAP PENYAKIT BERCAK DAUN HITAM PADA KACANG TANAH

A field evaluation was conducted to study the genotypic correlation between yield potentials and resistance level to the late leafspot of peanut. The experiment was carried out at Muara experimental-field from October 1994 to January 1995 using 100 genotypes with natural late leafspot inoculation. The result showed that the resistance level to late leafspot, wish was quantitatively reflected by the fresh-leaves percentage has negative genotypic correlation with total and filled-pod numbe1: The negative genotypic correlation were also found between resistance level and the weight of total pod, filled pod, as kernel, although not significant