Thiol stabilized copper nanoparticles exert antimicrobial properties by preventing cell division in Escherichia coli

The uses of metallic nanoparticles have gained importance as one of the therapeutic options to treat infections. Here, we synthesized stable copper nanoparticles (CuNPs) using Thiosalicylic acid and assessed their antimicrobial activities against various Gram-negative bacteria. The synthesized CuNPs had absorption maxima of 570 nM with a size range of 5-11 nM and face-centred cubic (Fcc) crystal structure. The bacterial cells in their planktonic and sessile forms were susceptible to CuNPs. The nanoparticles did not show any cytotoxicity to murine macrophages (RAW264.7) below 60 µg/mL. However, the expression of oxidative stress defence gene ahpC revealed the possibility of ROS generation upon treatment with CuNPs. Interestingly, the cell division proteins like, FtsZ and FtsI were destabilized in the presence of CuNPs which in turn inhibited bacterial cell division. In conclusion, it may be stated that the synthesized CuNPs can kill bacteria by arresting cell division and/or by ROS generation

Thiol stabilized copper nanoparticles exert antimicrobial properties by preventing cell division in Escherichia coli

151-157The uses of metallic nanoparticles have gained importance as one of the therapeutic options to treat infections. Here, we synthesized stable copper nanoparticles (CuNPs) using Thiosalicylic acid and assessed their antimicrobial activities against various Gram-negative bacteria. The synthesized CuNPs had absorption maxima of 570 nM with a size range of 5-11 nM and face-centred cubic (Fcc) crystal structure. The bacterial cells in their planktonic and sessile forms were susceptible to CuNPs. The nanoparticles did not show any cytotoxicity to murine macrophages (RAW264.7) below 60 µg/mL. However, the expression of oxidative stress defence gene ahpC revealed the possibility of ROS generation upon treatment with CuNPs. Interestingly, the cell division proteins like, FtsZ and FtsI were destabilized in the presence of CuNPs which in turn inhibited bacterial cell division. In conclusion, it may be stated that the synthesized CuNPs can kill bacteria by arresting cell division and/or by ROS generation

Improvement of neuropathology and transcriptional deficits in CAG 140 knock-in mice supports a beneficial effect of dietary curcumin in Huntington's disease

<p>Abstract</p> <p>Backgound</p> <p>No disease modifying treatment currently exists for Huntington's disease (HD), a fatal neurodegenerative disorder characterized by the formation of amyloid-like aggregates of the mutated huntingtin protein. Curcumin is a naturally occurring polyphenolic compound with Congo red-like amyloid binding properties and the ability to cross the blood brain barrier. CAG140 mice, a knock-in (KI) mouse model of HD, display abnormal aggregates of mutant huntingtin and striatal transcriptional deficits, as well as early motor, cognitive and affective abnormalities, many months prior to exhibiting spontaneous gait deficits, decreased striatal volume, and neuronal loss. We have examined the ability of life-long dietary curcumin to improve the early pathological phenotype of CAG140 mice.</p> <p>Results</p> <p>KI mice fed a curcumin-containing diet since conception showed decreased huntingtin aggregates and increased striatal DARPP-32 and D1 receptor mRNAs, as well as an amelioration of rearing deficits. However, similar to other antioxidants, curcumin impaired rotarod behavior in both WT and KI mice and climbing in WT mice. These behavioral effects were also noted in WT C57Bl/6 J mice exposed to the same curcumin regime as adults. However, neither locomotor function, behavioral despair, muscle strength or food utilization were affected by curcumin in this latter study. The clinical significance of curcumin's impairment of motor performance in mice remains unclear because curcumin has an excellent blood chemistry and adverse event safety profile, even in the elderly and in patients with Alzheimer's disease.</p> <p>Conclusion</p> <p>Together with this clinical experience, the improvement in several transgene-dependent parameters by curcumin in our study supports a net beneficial effect of dietary curcumin in HD.</p

A simple synthesis of hydroxyisoflavanones

142-144The isoflavanoids 7-hydroxyisoflavanone 4a, 7-hydroxy-3', 4'-dimethoxyisoflavanone 4b, 5, 7-dihydroxy-3', 4'-dimethoxyisoflavanone 4c, 5, 7-dihydroxy-2'-methoxyisoflavanone 4d, 5, 7-dihydroxy-3', 4'-dimethoxyisoflavanone 4e and 5,7-dihydroxy 4'-methoxyisoflavano ne 4f have been conveniently prepared by using paraforomaldehyde and diethylamine as a base

An Investigation to Assess the Cause of Accident due to Flyrockin an Opencast Coal Mine: A Case Study

Abstract The paper deals with the investigation carried out to find out the possible reason of the fly rock incident causing fatality of one person at Bhurkunda ‘A´’ Colliery of Central Coalfields Limited (CCL). The flying fragment travelled up to 280 m from the blasting source causing the accidental death. The blasts were conducted in over burden rock strata consisting of medium grained sandstone and shale. The blast hole diameter used in the blast was160 mm, and the average hole depth was 6.0 m. Site mixed emulsion (SME) explosive was used in the blast, and explosive charge per hole was 50.00 kg. Non-electric initiation systems were used for in-hole explosive and surface hole-to-hole initiations. The design parameters used in the blast were thoroughly analyzed using blast simulation software to check any anomaly in the blast design viz. sequence of hole firing, success rate of burden movement, maximum charge per delay fired within 8ms windows, etc. The different fly rock fragments prediction models were used to assess the maximum possible travel distance of the flying in the blast. A synonymous blast was also conducted in the mines to replicate the blast where fly rock accident occurred. All the blasting events were recorded and monitored using a digital video camera. The simulation results of the blast showed the success rate of burden relief more than 80% with sufficient delay intervals for the rock movement during the blast. The maximum possible travel distance of flying fragments based on different fly rock prediction models was 227 m. In the synonymous blast only vertical throws of the flying fragments up to 70 m(approximate) height were observed. It was difficult to find out the exact cause of fly rock incident. However, based on the detailed investigation, it was concluded that the possible cause of flying fragments travelling up to a distance of280 m could be due to the presence of a weak zone in the rock strat

Fundamental understanding of the size and surface modification effects on r(1), the relaxivity of Prussian blue nanocube@m-SiO2: a novel targeted chemo-photodynamic theranostic agent to treat colon cancer

A targeted multimodal strategy on a single nanoplatform is attractive in the field of nanotheranostics for the complete ablation of cancer. Herein, we have designed mesoporous silica (m-SiO2)-coated Prussian blue nanocubes (PBNCs), functionalized with hyaluronic acid (HA) to construct a multifunctional PBNC@m-SiO2@HA nanoplatform that exhibited good biocompatibility, excellent photodynamic activity, and in vitro T-1-weighted magnetic resonance imaging ability (r(1) similar to 3.91 mM(-1) s(-1)). After loading doxorubicin into the as-prepared PBNC@m-SiO2@HA, the developed PBNC@m-SiO2@HA@DOX displayed excellent pH-responsive drug release characteristics. Upon irradiation with 808 nm (1.0 W cm(-2)) laser light, PBNC@m-SiO2@HA@DOX exhibited synergistic photodynamic and chemotherapeutic efficacy (similar to 78% in 20 minutes) for human colorectal carcinoma (HCT 116) cell line compared to solo photodynamic or chemotherapy. Herein, the chemo-photodynamic therapeutic process was found to follow the apoptotic pathway via ROS-mediated mitochondrion-dependent DNA damage with a very low cellular uptake of PBNC@m-SiO2@HA@DOX for the human embryonic kidney (HEK 293) cell line, illustrating its safety. Hence, it may be stated that the developed nanoplatform can be a potential theranostic agent for future applications. Most interestingly, we have noted variation in r(1) at each step of the functionalization along with size variation that has been the first time modelled on the basis of the Solomon-Bloembergen-Morgan theory considering changes in the defect crystal structure, correlation time, water diffusion rate, etc., due to varied interactions between PBNC and water molecules

Level and Associated Factors of Literacy and Stigma of Suicide among Bangladeshi Physicians: A Cross-Sectional Assessment

Objectives. Literacy and stigma of suicide among doctors affect health-service delivery for persons with suicidal behavior. However, no attempt has been identified to assess those among physicians in Bangladesh. We aimed to determine the level and associated factors of suicide literacy and stigma toward suicide among physicians in Bangladesh. Methods. We collected data from 203 physicians in February 2022 by Google Forms. We used the Bangla literacy of suicide scale (LOSS-B) and the Bangla stigma of suicide scale (SOSS-B) to assess the literacy and stigma of suicide. The instrument also included questions for collecting sociodemographic variables and assessing suicidal behavior. Results. The mean age of the physicians was 34.17±7.86 (range 23–66) years, 109 (53.7%) were females, 150 (73.9%) were married, and 181 (89.2%) were Muslim. The mean LOSS-B score was 5.9±1.96 (range 1-10). Suicide literacy was higher in singles (p=0.013), doctors with a family history of suicide (p=0.018), a history of suicidal thought in lifetime (p≤0.001), and in the last year (p=0.03). Muslims (p=0.017) and city dwellers (p=0.021) had higher scores in the stigma subscale of SOSS-B whilst respondents with history of mental illness had a significantly lower level of stigma (p≤0.006). The stigma and isolation subscales were positively correlated indicating a higher value stigma creates higher isolation (p≤0.001). No relationship between suicide literacy and suicide stigma was identified among the physicians. Conclusions. Suicide literacy among the physicians of Bangladesh is low albeit higher than the level among the students. Appropriate programs should be designed to improve the status quo because physicians play fundamental roles as health-service providers as well as gatekeepers in suicide prevention