The Use of Methanol-Grown Yeast LI-70 in Feeds for Broilers

Abstract In 60-day feeding trials, broilers were fed commercial diets in which different amounts of methanol-grown yeast LI-70 replaced fish and soybean meal. In the first trial, all-mash diets containing up to 15% yeast produced growth rates and efficiencies of feed conversion almost equal to those of the soybean meal control and slightly below those of the fish meal control. In the second trial, pelleted diets containing up to 25% yeast were used. For yeast levels up to 15%, growth rates were faster than for the soybean meal control and slightly slower than for the fish meal control. Diets with more than 15% yeast lacked selenium. Diets containing 25% yeast as the sole source of protein but supplemented with .3 ppm selenium produced growth rates and efficiencies of feed conversion equal to those of the controls

Development of a semi-automated procedure for high throughput screening of microbial sensitivity to inhibitory substances

A semi-automated procedure for the evaluation of microbial sensitivity to biocides and antibiotics was developed in a 384 well plates. The procedure allow to test simultaneously 16 strains in triplicate against six different concentration of an antimicrobial substance. The coupling of a 384 well plate micro-dilution method with a spectrophotometer evaluation of the cellular density allowed the measurement of growth kinetic parameters useful to describe the microbial growth response in presence of different concentrations of antimicrobials. The semi-automated procedure was applied to evaluate the sensitivity of 200 lactic acid bacteria strains of dairy and non-dairy origin towards four biocides and four antibiotics. Moreover, the versatility of the procedure developed was useful to characterize the phenotype of S. thermophilus mutants showing a reduced susceptibility to chlorhexidine and ethidium bromide by the over expression of pmrAB multidrug resistance efflux pump system

Development of a semi-automated procedure for high throughput screening of microbial sensitivity to inhibitory substances

A semi-automated procedure for the evaluation of microbial sensitivity to biocides and antibiotics was developed in a 384 well plates. The procedure allow to test simultaneously 16 strains in triplicate against six different concentration of an antimicrobial substance. The coupling of a 384 well plate micro-dilution method with a spectrophotometer evaluation of the cellular density allowed the measurement of growth kinetic parameters useful to describe the microbial growth response in presence of different concentrations of antimicrobials. The semi-automated procedure was applied to evaluate the sensitivity of 200 lactic acid bacteria strains of dairy and non-dairy origin towards four biocides and four antibiotics. Moreover, the versatility of the procedure developed was useful to characterize the phenotype of S. thermophilus mutants showing a reduced susceptibility to chlorhexidine and ethidium bromide by the over expression of pmrAB multidrug resistance efflux pump system