Effect of 131I-Labelling on Various Properties of Nonmitochondrial Aspartate Transaminase ftom Pig Heart

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Endoscopic findings in patients with achlorhydria, with special consideration to intestinal fungal colonization, xanthoma and polypoid lesions

L'endoscopie de routine de 88 patients achlorhydriques a révélé une incidence plus élevée de la colonisation mycotique de la muqueuse duodénale par rapport à des sujets témoins (40 % contre 6 %). Des xanthomes gastriques ont été observés chez 4 patients achlorhydriques. La fréquence des lésions polypoïdes chez les sujets achlorhydriques de plus de 50 ans (12,1 %) est la même que le groupe des patients témoins exempts de lésions gastriques (11,1 %), mais est supérieure à celle des patients porteurs d'ulcères peptiques (0,5%)

Preparative SDS-PAGE Electroelution for Rapid Purification of Alkyl Hydroperoxide Reductase from Helicobacter pylori

"nBackground: Alkyl hydroperoxide reductase (AhpC) of Helicobacter pylori is considered as a diagnostic antigen. There­fore, this antigen can be used to detect H. pylori infection by stool immunoassays such as ELISA. The aim of this study was to simplify the AhpC protein purification procedures."nMethods: For whole cell protein extraction, the bacterial cells were ruptured by octly-β-D glucopyranoside. The isolation and purification of  AhpC protein were attempted by various techniques including ammonium sulfate precipitation, dialysis, preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electroelution."nResults:A simple method was used for protein purification AhpC protein. One-dimensional preparative gel electrophoresis allows a single and short purification step; the high resolution capacity of this technique leads to a high level of purity of the protein. Moreover, it avoids contamination by other non-specific proteins which often appear during protein purification by column chromatography."nConclusion: The present method is simple, rapid and makes it possible to preparate AhpC from H. pylor