Efficacy of metabarcoding for identification of fish eggs evaluated with mock communities.

There is urgent need for effective and efficient monitoring of marine fish populations. Monitoring eggs and larval fish may be more informative than that traditional fish surveys since ichthyoplankton surveys reveal the reproductive activities of fish populations, which directly impact their population trajectories. Ichthyoplankton surveys have turned to molecular methods (DNA barcoding & metabarcoding) for identification of eggs and larval fish due to challenges of morphological identification. In this study, we examine the effectiveness of using metabarcoding methods on mock communities of known fish egg DNA. We constructed six mock communities with known ratios of species. In addition, we analyzed two samples from a large field collection of fish eggs and compared metabarcoding results with traditional DNA barcoding results. We examine the ability of our metabarcoding methods to detect species and relative proportion of species identified in each mock community. We found that our metabarcoding methods were able to detect species at very low input proportions; however, levels of successful detection depended on the markers used in amplification, suggesting that the use of multiple markers is desirable. Variability in our quantitative results may result from amplification bias as well as interspecific variation in mitochondrial DNA copy number. Our results demonstrate that there remain significant challenges to using metabarcoding for estimating proportional species composition; however, the results provide important insights into understanding how to interpret metabarcoding data. This study will aid in the continuing development of efficient molecular methods of biological monitoring for fisheries management

Ks-band (2.14 micron) imaging of southern massive star formation regions traced by methanol masers

We present deep, wide-field, Ks-band (2.14 micron) images towards 87 southern massive star formation regions traced by methanol maser emission. Using point-spread function fitting, we generate 2.14 micron point source catalogues towards each of the regions. For the regions between 10 degrees and 350 degrees galactic longitude and galactic latitude +/- 1 degree, we match the 2.14 micron sources with the GLIMPSE point source catalogue to generate a combined 2.14 to 8.0 micron point source catalogue. We provide this data for the astronomical community to utilise in studies of the stellar content of embedded clusters.Comment: Accepted PASA. Full version including figures available from http://www.cfa.harvard.edu/~slongmor/snl_iris2_withfigs.pd

Do Start-Ups Pay Less?

The authors analyze Danish registry data from 1991 to 2006 to determine how firm age and firm size influence wages. Unadjusted statistics suggest that smaller firms paid less than larger firms paid, and that firm age had little or no bearing on wages. After adjusting for differences in the characteristics of employees hired by these firms, however, they observe both firm age and firm size effects. Larger firms paid more than did smaller firms for observationally equivalent individuals but, contrary to conventional wisdom, younger firms paid more than older firms. The size effect, however, dominates the age effect. Thus, although the typical start-up — being both young and small — paid less than a more established employer, the largest start-ups paid a wage premium

High Spectral and Spatial Resolution Observations of Shocked Molecular Hydrogen at the Galactic Center

The presence of OH (1720 MHz) masers, and the absence of counterparts at 1665/1667 MHz has proved to be a clear diagnostic of shocked molecular gas associated with Galactic supernova remnants. This suggests that shocked molecular gas should be associated with the OH (1720 MHz) masers that have been detected in the circumnuclear disk (CND) and Sgr A East at the Galactic center. In order to test this hypothesis, we observed the H$_2$ 1--0 S(1) and Br $\gamma$ lines using NICMOS on the HST and UNSWIRF on the AAT, near the regions where OH (1720 MHz) masers are detected in the CND and Sgr A East. We present the distribution of H$_2$ in the North and South lobes of the CND and in Sgr A East. H$_2$ emission accompanies almost all of the maser spots detected at the Galactic center. In particular, we find a striking filamentary structure near the Northwest of the CND and evidence that shocked molecular gas is associated with the 70 \kms molecular cloud at the Galactic center. We argue that the emission from the CND could arise in gas heated by the dissipation of the random motion of clumps by collisions or the dissipation of turbulence in a more homogeneous medium. In addition, highly red-shifted gas of up to 140 \kms\ close to the eastern edge of the Sgr A East shell is detected. These observations combined with OH (1720 MHz) results suggest that the H$_2$ gas is shocked and accelerated by the expansion of Sgr A East into the 50 and the 70 \kms cloud and into the lobes of the CND.Comment: 31 pages plus 14 figures, ApJ (in press

Palatini Variational Principle for NN-Dimensional Dilaton Gravity

We consider a Palatini variation on a general $N$-Dimensional second order, torsion-free dilaton gravity action and determine the resulting equations of motion. Consistency is checked by considering the restraint imposed due to invariance of the matter action under simple coordinate transformations, and the special case of N=2 is examined. We also examine a sub-class of theories whereby a Palatini variation dynamically coincides with that of the "ordinary" Hilbert variational principle; in particular we examine a generalized Brans-Dicke theory and the associated role of conformal transformations.Comment: 16 pages, LaTe

Ceramic composition at Chalcolithic Shiqmim, northern Negev desert, Israel: investigating technology and provenance using thin section petrography, instrumental geochemistry and calcareous nannofossils

Technological innovations in ceramic production and other crafts are hallmarks of the Chalcolithic period (4500–3600 BCE) in the southern Levant, but details of manufacturing traditions have not been fully investigated using the range of analytical methods currently available. This paper presents results of a compositional study of 51 sherds of ceramic churns and other pottery types from the Chalcolithic site of Shiqmim in the northern Negev desert. By applying complementary thin section petrography, instrumental geochemistry and calcareous nannofossil analyses, connections between the raw materials, clay paste recipes and vessel forms of the selected ceramic samples are explored and documented. The study indicates that steps in ceramic manufacturing can be related to both technological choices and local geology. Detailed reporting of the resulting data facilitates future comparative ceramic compositional research that is needed as a basis for testable regional syntheses and to better resolve networks of trade/exchange and social group movement

The inexorable resistance of inertia determines the initial regime of drop coalescence

Drop coalescence is central to diverse processes involving dispersions of drops in industrial, engineering and scientific realms. During coalescence, two drops first touch and then merge as the liquid neck connecting them grows from initially microscopic scales to a size comparable to the drop diameters. The curvature of the interface is infinite at the point where the drops first make contact, and the flows that ensue as the two drops coalesce are intimately coupled to this singularity in the dynamics. Conventionally, this process has been thought to have just two dynamical regimes: a viscous and an inertial regime with a crossover region between them. We use experiments and simulations to reveal that a third regime, one that describes the initial dynamics of coalescence for all drop viscosities, has been missed. An argument based on force balance allows the construction of a new coalescence phase diagram

Constructing sonified haptic line graphs for the blind student: first steps

Line graphs stand as an established information visualisation and analysis technique taught at various levels of difficulty according to standard Mathematics curricula. It has been argued that blind individuals cannot use line graphs as a visualisation and analytic tool because they currently primarily exist in the visual medium. The research described in this paper aims at making line graphs accessible to blind students through auditory and haptic media. We describe (1) our design space for representing line graphs, (2) the technology we use to develop our prototypes and (3) the insights from our preliminary work

Research in COVID-19 times: The way forward

The COVID-19 pandemic has had a major impact on research at universities. Universities around the world, including in South Africa, have been or are still closed as part of national lockdown strategies. Students have not been attending classes or doing hands-on experimental work, and students and academics have been working from home. Many thousands of students have had their university education interrupted, and for them, the resumption of learning programmes online, and where possible in research laboratories, is critically important. There is no question that as we emerge from lockdown we will not be entering a world that resembles a ‘norm’ as lived in the pre-COVID-19 era, and many changes will be required. Here we discuss the importance of research, the urgency to get things up and running again, and strategies that will need to be implemented to ensure that research activities continue. At the same time, it is necessary to ensure that students and staff are not exposed to risk in their research endeavours, which will require the development and implementation of risk management plans