Conspicuous Smooth and White Egg-Shaped Sulfur Structures on a Deep-Sea Hydrothermal Vent Formed by Sulfide-Oxidizing Bacteria

Conspicuous egg-shaped, white, and smooth structures were observed at a hydrothermal vent site in the Guaymas Basin, Gulf of California. The gelatinous structures decomposed within hours after sampling. Scanning electron microscopy (SEM) and light microscopy showed that the structure consisted of filaments of less than 0.1 mm thickness, similar to those observed for "Candidatus Arcobacter sulfidicus." SEM-energy-dispersive X-ray spectroscopy (EDS) showed that the filaments were sulfur rich. According to 16S rRNA gene amplicon and fluorescence in situ hybridization (FISH) analyses, Arcobacter, a sulfide oxidizer that is known to produce filamentous elemental sulfur, was among the dominant species in the structure and was likely responsible for its formation. Arcobacter normally produces woolly snowflake like structures in opposed gradients of sulfide and oxygen. In the laboratory, we observed sulfide consumption in the anoxic zone of the structure, suggesting an anaerobic conversion. The sulfide oxidation and decomposition of the structure in the laboratory may be explained by dissolution of the sulfur filaments by reaction with sulfide under formation of polysulfides. IMPORTANCE At the deep-sea Guaymas Basin hydrothermal vent system, sulfide-rich hydrothermal fluids mix with oxygenated seawater, thereby providing a habitat for microbial sulfur oxidation. Microbial sulfur oxidation in the deep sea involves a variety of organisms and processes and can result in the excretion of elemental sulfur. Here, we report on conspicuous white and smooth gelatinous structures found on hot vents. These strange egg-shaped structures were often observed on previous occasions in the Guaymas Basin, but their composition and formation process were unknown. Our data suggest that the notable and highly ephemeral structure was likely formed by the well-known sulfide-oxidizing Arcobacter. While normally Arcobacter produces loose flocs or woolly layers, here smooth gel-like structures were found

The rate and fate of N-2 and C fixation by marine diatom-diazotroph symbioses

N-2 fixation constitutes an important new nitrogen source in the open sea. One group of filamentous N-2 fixing cyanobacteria (Richelia intracellularis, hereafter Richelia) form symbiosis with a few genera of diatoms. High rates of N-2 fixation and carbon (C) fixation have been measured in the presence of diatom-Richelia symbioses. However, it is unknown how partners coordinate C fixation and how the symbiont sustains high rates of N-2 fixation. Here, both the N-2 and C fixation in wild diatom-Richelia populations are reported. Inhibitor experiments designed to inhibit host photosynthesis, resulted in lower estimated growth and depressed C and N-2 fixation, suggesting that despite the symbionts ability to fix their own C, they must still rely on their respective hosts for C. Single cell analysis indicated that up to 22% of assimilated C in the symbiont is derived from the host, whereas 78-91% of the host N is supplied from their symbionts. A size-dependent relationship is identified where larger cells have higher N-2 and C fixation, and only N-2 fixation was light dependent. Using the single cell measures, the N-rich phycosphere surrounding these symbioses was estimated and contributes directly and rapidly to the surface ocean rather than the mesopelagic, even at high estimated sinking velocities (<10 m d(-1)). Several eco-physiological parameters necessary for incorporating symbiotic N-2 fixing populations into larger basin scale biogeochemical models (i.e., N and C cycles) are provided

Biofilms on glacial surfaces: hotspots for biological activity

Glaciers are important constituents in the Earth’s hydrological and carbon cycles, with predicted warming leading to increases in glacial melt and the transport of nutrients to adjacent and downstream aquatic ecosystems. Microbial activity on glacial surfaces has been linked to the biological darkening of cryoconite particles, affecting albedo and increased melt. This phenomenon, however, has only been demonstrated for alpine glaciers and the Greenland Ice Sheet, excluding Antarctica. In this study, we show via confocal laser scanning microscopy that microbial communities on glacial surfaces in Antarctica persist in biofilms. Overall, ~35% of the cryoconite sediment surfaces were covered by biofilm. Nanoscale scale secondary ion mass spectrometry measured significant enrichment of 13C and 15N above background in both Bacteroidetes and filamentous cyanobacteria (i.e., Oscillatoria) when incubated in the presence of 13C–NaHCO3 and 15NH4. This transfer of newly synthesised organic compounds was dependent on the distance of heterotrophic Bacteroidetes from filamentous Oscillatoria. We conclude that the spatial organisation within these biofilms promotes efficient transfer and cycling of nutrients. Further, these results support the hypothesis that biofilm formation leads to the accumulation of organic matter on cryoconite minerals, which could influence the surface albedo of glaciers

Direct cell mass measurements expand the role of small microorganisms in nature.

Microbial biomass is a key parameter needed for the quantification of microbial turnover rates and their contribution to the biogeochemical element cycles. However, estimates of microbial biomass rely on empirically-derived mass-to-volume relationships and large discrepancies exist between the available empirical conversion factors. Here we report a significant non-linear relationship between carbon mass and cell volume (mcarbon = 197 × V0.46.; R2 = 0.95) based on direct cell mass, volume and elemental composition measurements of twelve prokaryotic species with average volumes between 0.011 – 0.705 μm3. The carbon mass density of our measured cells ranged from 250 to 1800 fg C μm-3 for the measured cell volumes. Compared to other currently used models, our relationship yielded up to 300 % higher carbon mass values. A compilation of our and previously published data showed that cells with larger volumes (> 0.5 μm3) display a constant (carbon) mass-to-volume ratio whereas cells with volumes below 0.5 μm3 exhibit a nonlinear increase in (carbon) mass density with decreasing volume. Small microorganisms dominate marine and freshwater bacterioplankton as well as soils and marine and terrestrial subsurface. The application of our experimentally-determined conversion factors will help to quantify the true contribution of these microorganisms to ecosystem functions and global microbial biomass

A conceptual basis for surveying fouling communities at exposed and protected sites at sea: Feasible designs with exchangeable test bodies for in-situ biofouling collection

The enhanced inertia load caused by biofouling on device components, such as the foundations of wind turbines or other structures at sea, modifies the hydrodynamic properties, and increases the stress to structures, predominantly in upper water layers with high impact from wave dynamics. This compromises the stability, functioning, operation as well as the durability of these devices especially in exposed environments. A main challenge is the quantification of the impact of hydrodynamic forces on irregular bodies being overgrown by soft- and hard-bodied biofouling organisms. Therefore, test bodies from the upper 1–5 m water depth and thus exposed to the strongest wave actions close to the surface shall be overgrown by biofouling and used in measurement trials in a wave and current flume. These measurements shall shed light on the varying roughness and its influence on the load bearing capacity of foundation piles. Consequently, the main aims of the present work were the development of two independent test stations as holding devices for artificial test bodies for the collection of biofouling organisms during field studies: a carrying unit floating at the surface in an exposed area (System A) and a sampling device with access from a land-based facility (System B). Both systems are relatively easy to access, exhibit straightforward handling, and are reasonable cost-effective. A Test Body Support Unit (TBSU, System A) was designed and mounted on a spare buoy to carry the test bodies (cylinders), which serve as substrate for the fouling. The system was sufficiently robust to withstand several periods of rough sea conditions over the first two years. This system can only be accessed by vessels. System B (MareLift) provided the robustness and functionality needed for areas exhibiting harsh conditions but can be operated from land. The here used test bodies (steel panels) exhibited a sound basis for the monitoring of succession processes in the biofouling development. System B offered the possibility to analyse two habitats (intertidal and subtidal) and revealed clear differences in the composition and development of their fouling communities. Overall, both systems provide advantages in obtaining standardized biofouling samples compared to previous approaches. Such test stations play an important role in the risk management of marine sectors as they could help characterising biofouling communities over different geographical areas. System A and B provide a sound basis for biofouling research but potentially also for other potential research approaches in exposed areas as they provide space for future developments

Nitrate respiration and diel migration patterns of diatoms are linked in sediments underneath a microbial mat

Diatoms are among the few eukaryotes known to store nitrate (NO3−) and to use it as an electron acceptor for respiration in the absence of light and O2. Using microscopy and 15N stable isotope incubations, we studied the relationship between dissimilatory nitrate/nitrite reduction to ammonium (DNRA) and diel vertical migration of diatoms in phototrophic microbial mats and the underlying sediment of a sinkhole in Lake Huron (USA). We found that the diatoms rapidly accumulated NO3− at the mat-water interface in the afternoon and 40% of the population migrated deep into the sediment, where they were exposed to dark and anoxic conditions for ~75% of the day. The vertical distribution of DNRA rates and diatom abundance maxima coincided, suggesting that DNRA was the main energy generating metabolism of the diatom population. We conclude that the illuminated redox-dynamic ecosystem selects for migratory diatoms that can store nitrate for respiration in the absence of light. A major implication of this study is that the dominance of DNRA over denitrification is not explained by kinetics or thermodynamics. Rather, the dynamic conditions select for migratory diatoms that perform DNRA and can outcompete sessile denitrifiers

Responses of the coastal bacterial community to viral infection of the algae <i>Phaeocystis globosa</i>

The release of organic material upon algal cell lyses has a key role in structuring bacterial communities and affects the cycling of biolimiting elements in the marine environment. Here we show that already before cell lysis the leakage or excretion of organic matter by infected yet intact algal cells shaped North Sea bacterial community composition and enhanced bacterial substrate assimilation. Infected algal cultures of Phaeocystis globosa grown in coastal North Sea water contained gamma-and alphaproteobacterial phylotypes that were distinct from those in the non-infected control cultures 5 h after infection. The gammaproteobacterial population at this time mainly consisted of Alteromonas sp. cells that were attached to the infected but still intact host cells. Nano-scale secondary-ion mass spectrometry (nanoSIMS) showed similar to 20% transfer of organic matter derived from the infected C-13- and N-15-labelled P. globosa cells to Alteromonas sp. cells. Subsequent, viral lysis of P. globosa resulted in the formation of aggregates that were densely colonised by bacteria. Aggregate dissolution was observed after 2 days, which we attribute to bacteriophage-induced lysis of the attached bacteria. Isotope mass spectrometry analysis showed that 40% of the particulate C-13-organic carbon from the infected P. globosa culture was remineralized to dissolved inorganic carbon after 7 days. These findings reveal a novel role of viruses in the leakage or excretion of algal biomass upon infection, which provides an additional ecological niche for specific bacterial populations and potentially redirects carbon availability

Purple sulfur bacteria fix N-2 via molybdenum-nitrogenase in a low molybdenum Proterozoic ocean analogue

Biological N-2 fixation was key to the expansion of life on early Earth. The N-2-fixing microorganisms and the nitrogenase type used in the Proterozoic are unknown, although it has been proposed that the canonical molybdenum-nitrogenase was not used due to low molybdenum availability. We investigate N-2 fixation in Lake Cadagno, an analogue system to the sulfidic Proterozoic continental margins, using a combination of biogeochemical, molecular and single cell techniques. In Lake Cadagno, purple sulfur bacteria (PSB) are responsible for high N-2 fixation rates, to our knowledge providing the first direct evidence for PSB in situ N-2 fixation. Surprisingly, no alternative nitrogenases are detectable, and N-2 fixation is exclusively catalyzed by molybdenum-nitrogenase. Our results show that molybdenum-nitrogenase is functional at low molybdenum conditions in situ and that in contrast to previous beliefs, PSB may have driven N-2 fixation in the Proterozoic ocean. N-2 fixation was key to the expansion of life on Earth, but which organisms fixed N-2 and if Mo-nitrogenase was functional in the low Mo early ocean is unknown. Here, the authors show that purple sulfur bacteria fix N-2 using Mo-nitrogenase in a Proterozoic ocean analogue, despite low Mo conditions

Single cell analyses reveal contrasting life strategies of the two main nitrifiers in the ocean

Nitrification, the oxidation of ammonia via nitrite to nitrate, is a key process in marine nitrogen (N) cycling. Although oceanic ammonia and nitrite oxidation are balanced, ammonia-oxidizing archaea (AOA) vastly outnumber the main nitrite oxidizers, the bacterial Nitrospinae. The ecophysiological reasons for this discrepancy in abundance are unclear. Here, we compare substrate utilization and growth of Nitrospinae to AOA in the Gulf of Mexico. Based on our results, more than half of the Nitrospinae cellular N-demand is met by the organic-N compounds urea and cyanate, while AOA mainly assimilate ammonium. Nitrospinae have, under in situ conditions, around four-times higher biomass yield and five-times higher growth rates than AOA, despite their ten-fold lower abundance. Our combined results indicate that differences in mortality between Nitrospinae and AOA, rather than thermodynamics, biomass yield and cell size, determine the abundances of these main marine nitrifiers. Furthermore, there is no need to invoke yet undiscovered, abundant nitrite oxidizers to explain nitrification rates in the ocean