Theoretical and experimental study of a new method for prediction of profile drag of airfoil sections

Theoretical and experimental studies are described which were conducted for the purpose of developing a new generalized method for the prediction of profile drag of single component airfoil sections with sharp trailing edges. This method aims at solution for the flow in the wake from the airfoil trailing edge to the large distance in the downstream direction; the profile drag of the given airfoil section can then easily be obtained from the momentum balance once the shape of velocity profile at a large distance from the airfoil trailing edge has been computed. Computer program subroutines have been developed for the computation of the profile drag and flow in the airfoil wake on CDC6600 computer. The required inputs to the computer program consist of free stream conditions and the characteristics of the boundary layers at the airfoil trailing edge or at the point of incipient separation in the neighborhood of airfoil trailing edge. The method described is quite generalized and hence can be extended to the solution of the profile drag for multi-component airfoil sections

Recent integral cross section validation measurements at the ASP facility

This work presents new integral data measured at the ASP 14 MeV neutron irradiation facility at Aldermaston in the UK, which has recently become available for fusion-related work through the CCFE materials programme. Measurements of reaction products from activation experiments using elemental foils were carried out using gamma spectrometry in a high efficiency, high-purity germanium (HPGe) detector and associated digital signal processing hardware. Following irradiation and rapid extraction to the measurement cell, gamma emissions were acquired with both energy and time bins. Integral cross section and half-life data have been derived from these measurements. Selected integral cross section values are presented from the measurement campaigns.Comment: 4 pages, 5 figure

Towards reproducible MSMS data preprocessing, quality control and quantification

The development of MSnbase aims at providing researchers dealing with labelled quantitative proteomics data with a transparent, portable, extensible and open-source collaborative framework to easily manipulate and analyse MS2-level raw tandem mass spectrometry data. The implementation in R gives users and developers a great variety of powerful tools to be used in a controlled and reproducible way. Furthermore, MSnbase has been developed following an object-oriented programming paradigm: all information that is manipulated by the user is encapsulated in ad hoc data containers to hide it's underlying complexity. We illustrate the usage and achievements of our software using a published spiked-in data set in which varying quantities of test proteins have been labelled with four different iTRAQ tags. In addition to providing raw MSMS data, MSnbase also stores meta-data and logs processing steps in the data object itself for optimal traceability. We provide graphics on how to inspect precursor data for quality control and how individual or merged MSMS spectra can subsequently be processed, plotted and extracted using a variety of methods. We also demonstrate how reporter ions (or any peaks of interest defined by the user) can easily be quantified and normalised using several build-in alternative strategies and how the effect of each transformation can be recorded, examined and reproduced. MSnbase constitutes a unique versatile working and development environment to process labelled MSMS data and provides in turn important feedback for data acquisition optimisation. We conclude by presenting future extensions of MSnbase and highlight its usage in reproducible proteomics research

Experimental study of the separating confluent boundary-layer. Volume 2: Experimental data

An experimental low speed study of the separating confluent boundary layer on a NASA GAW-1 high lift airfoil is described. The airfoil was tested in a variety of high lift configurations comprised of leading edge slat and trailing edge flap combinations. The primary test instrumentation was a two dimensional laser velocimeter (LV) system operating in a backscatter mode. Surface pressures and corresponding LV derived boundary layer profiles are given in terms of velocity components, turbulence intensities and Reynolds shear stresses as characterizing confluent boundary layer behavior up to and beyond stall. LV derived profiles and associated boundary layer parameters and those obtained from more conventional instrumentation such as pitot static transverse, Preston tube measurements and hot-wire surveys are compared

Two-dimensional features of correlations in the flow and near pressure fields of Mach number 0.9 jets

International audienceIn the present study, two-dimensional spatial correlations are calculated in the flow and the near pressure fields of two isothermal round jets at a Mach number of 0.9, computed by highly-resolved simulations using cylindrical coordinates (r , θ, z). The two jets have diameter-based Reynolds numbers of 3, 125 and 100, 000, and they are initially weakly and strongly disturbed, respectively. For both jets, correlations are evaluated between signals at a given point, namely flow fluctuations on the jet axis at the end of the potential core and pressure fluctuations in the jet near field, and 2-D fields acquired in sections (z, r). The full signals but also the axisymmetric and first azimuthal modes are considered. Overall, despite the significant differences in Reynolds number and nozzle-exit conditions, the results for the two jets are very similar. Strong levels of correlations are obtained over large spatial regions and long time periods, providing information on noise generation mechanisms. In particular, the 2-D correlation fields reveal the presence of a wavepacket-like structure growing in the jet mixing layers, centered on a correlation spot in the potential core, and peaking in amplitude around the end of the jet core, which leads to the emission of sound waves in the downstream direction

Comparison of dimethyl sulfoxide treated highly conductive poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) electrodes for use in indium tin oxide-free organic electronic photovoltaic devices

Indium tin oxide (ITO)-free organic photovoltaic (OPV) devices were fabricated using highly conductive poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) as the transparent conductive electrode (TCE). The intrinsic conductivity of the PEDOT:PSS films was improved by two different dimethyl sulfoxide (DMSO) treatments – (i) DMSO was added directly to the PEDOT:PSS solution (PEDOT:PSSADD) and (ii) a pre-formed PEDOT:PSS film was immersed in DMSO (PEDOT:PSSIMM). X-ray photoelectron spectroscopy (XPS) and conductive atomic force microscopy (CAFM) studies showed a large amount of PSS was removed from the PEDOT:PSSIMM electrode surface. OPV devices based on a poly(3-hexylthiophene):[6,6]-phenyl-C61-butyric acid methyl ester (P3HT:PCBM) bulk hetrojunction showed that the PEDOT:PSSIMM electrode out-performed the PEDOT:PSSADD electrode, primarily due to an increase in short circuit current density from 6.62 mA cm−2 to 7.15 mA cm−2. The results highlight the importance of optimising the treatment of PEDOT:PSS electrodes and demonstrate their potential as an alternative TCE for rapid processing and low-cost OPV and other organic electronic devices

The chicken B-cell line DT40 proteome, beadome and interactomes.

In developing a new quantitative AP-MS method for exploring interactomes in the chicken B-cell line DT40, we also surveyed the most abundant proteins in this organism and explored the likely contaminants that bind to a variety of affinity resins that would later be confirmed quantitatively [1]. We present the 'Top 150 abundant DT40 proteins list', the DT40 beadomes as well as protein interaction lists for the Phosphatidyl inositol 5-phosphate 4-kinase 2β and Fanconi anaemia protein complexes.We thanks Prof. R. Irvine for providing the JPR3 cell line, to Dr. E. Rajendra for providing the FANCC cell line, FANC antibodies and helpful discussions, to Dr. M. Deery and J. Howard for assistance with MS. This work was funded by the Biotechnology and Biological Sciences Research Council (UK) Grant BB/H024085/1.This is the final version of the article. It was first available from Elsevier via http://dx.doi.org/10.1016/j.dib.2014.12.00

Ciprofloxacin binding to GyrA causes global changes in the proteome of Pseudomonas aeruginosa.

Ciprofloxacin is one of the most widely-used antibiotics, and has proven especially effective at controlling infections associated with the opportunistic human pathogen, Pseudomonas aeruginosa. In this work, we show that sub-inhibitory concentrations of ciprofloxacin induce discrete changes in the intracellular proteome. Central metabolism and cell envelope-associated functions are particularly affected. In spite of the low magnitude of the intracellular proteomic changes, we found that sub-lethal concentrations of ciprofloxacin had substantial effects on motility and exoprotein secretion. Crucially, the proteomic and phenotypic modulations that we observed were absolutely dependent upon the presence of wild-type GyrA; an isogenic strain of P. aeruginosa carrying a ciprofloxacin-insensitive form of GyrA (a T83→I mutant) did not display ciprofloxacin-dependent changes unless complemented with wild-type gyrA in trans. These results show that the diverse effects of sub-inhibitory ciprofloxacin on the cell are routed through its primary target in the cell, DNA gyrase.This work was supported by BBSRC grants BB/C500252/1 and BB/M019411/1

Wrapping Transition and Wrapping-Mediated Interactions for Discrete Binding along an Elastic Filament: An Exact Solution

The wrapping equilibria of one and two adsorbing cylinders are studied along a semi-flexible filament (polymer) due to the interplay between elastic rigidity and short-range adhesive energy between the cylinder and the filament. We show that statistical mechanics of the system can be solved exactly using a path integral formalism which gives access to the full effect of thermal fluctuations, going thus beyond the usual Gaussian approximations which take into account only the contributions from the minimal energy configuration and small fluctuations about this minimal energy solution. We obtain the free energy of the wrapping-unwrapping transition of the filament around the cylinders as well as the effective interaction between two wrapped cylinders due to thermal fluctuations of the elastic filament. A change of entropy due to wrapping of the filament around the adsorbing cylinders as they move closer together is identified as an additional source of interactions between them. Such entropic wrapping effects should be distinguished from the usual entropic configuration effects in semi-flexible polymers. Our results may be applicable to the problem of adsorption of proteins as well as synthetic nano-particles on semi-flexible polymers such as DNA.Comment: 24 pages, 12 figure

Amino acid sequence of conglutin δ, a sulfur-rich seed protein of Lupinus angustifolius L. Sequence homology with the C-III α-amylase inhibitor from wheat

AbstractComplete amino acid sequences and disulfide cross-link arrangements have been determined for the two subunit polypeptides (Mr 9401 and 4597) ofconglutin δ, a helix-rich seed protein from Lupinus angustifolius cv. Uniwhite. There are two intrachain disulfide bonds and a free sulfhydryl group within the large chain and two interchain disulfide bonds to the small chain. The sequences show regions enriched in glutamineglutamic acid and serine residues which were correlated by a predictive method to the high measured level of α-helix (~ 38%). Homology was found between a cystine-rich region ofconglutin δ and the C-III α-amylase inhibitor from wheat suggesting that these proteins originated from a common ancestral gene