2,946 research outputs found

    Single amino acid (arginine) deprivation: rapid and selective death of cultured transformed and malignant cells

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    The effects of arginine deprivation (–Arg) has been examined in 26 cell lines. Less than 10% of those with transformed or malignant phenotype survived for > 5 days, and many died more rapidly, notably leukaemic cells. Bivariate flow cytometry confirmed that vulnerable cell lines failed to move out of cell cycle into a quiescent state (G0), but reinitiated DNA synthesis. Many cells remained in S-phase, and/or had difficulty progressing through to G2 and M. Two tumour lines proved relatively ‘resistant’, A549 and MCF7. Although considerable cell loss occurred initially, both lines showed a ‘cell cycle freeze’, in which cells survived for > 10 days. These cells recovered their proliferative activity in +Arg medium, but behaved in the same manner to a second –Arg episode as they did to the first episode. In contrast, normal cells entered G0 and survived in –Arg medium for several weeks, with the majority of cells recovering with predictable kinetics in +Arg medium. In general, cells from a wide range of tumours and established lines die quickly in vitro following –Arg treatment, because of defective cell cycle checkpoint stringency, the efficacy of the treatment being most clearly demonstrated in co-cultures in which only the normal cells survived. The findings demonstrate a potentially simple, effective and non-genotoxic strategy for the treatment of a wide range of cancers. © 2000 Cancer Research Campaig

    Employee satisfaction and use of flexible working arrangements

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    This article considers the impact of flexible working arrangements (FWAs), using the British Household Panel Survey and Understanding Society, 2001–10/11. Results of panel logit, ANCOVA and change-score analysis are indicative of positive impacts from use of a number of FWAs, including homeworking having positive effects for men and women on job and leisure satisfaction. However, findings reveal gaps in availability and use of FWAs, and highlight the gendered nature of flexible employment. Flexi-time, the most common FWA among men, has positive effects as it facilitates management of household responsibilities while maintaining full-time employment. Part-time and homeworking are also positive, consistent with men using FWAs with a greater degree of choice. Women more often are constrained in their use of FWAs, often into working reduced hours. Consequently, FWAs have negative impacts for some women, on job (part-time when used for extended periods, flexi-time), leisure (job-share, flexi-time) and life satisfaction (job-share)

    Magnetism, X-rays, and Accretion Rates in WD 1145+017 and other Polluted White Dwarf Systems

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    This paper reports circular spectropolarimetry and X-ray observations of several polluted white dwarfs including WD 1145+017, with the aim to constrain the behavior of disk material and instantaneous accretion rates in these evolved planetary systems. Two stars with previously observed Zeeman splitting, WD 0322-019 and WD 2105-820, are detected above 5 sigma and > 1 kG, while WD 1145+017, WD 1929+011, and WD 2326+049 yield (null) detections below this minimum level of confidence. For these latter three stars, high-resolution spectra and atmospheric modeling are used to obtain limits on magnetic field strengths via the absence of Zeeman splitting, finding B* < 20 kG based on data with resolving power R near 40 000. An analytical framework is presented for bulk Earth composition material falling onto the magnetic polar regions of white dwarfs, where X-rays and cyclotron radiation may contribute to accretion luminosity. This analysis is applied to X-ray data for WD 1145+017, WD 1729+371, and WD 2326+049, and the upper bound count rates are modeled with spectra for a range of plasma kT = 1 - 10 keV in both the magnetic and non-magnetic accretion regimes. The results for all three stars are consistent with a typical dusty white dwarf in a steady-state at 1e8 - 1e9 g/s. In particular, the non-magnetic limits for WD 1145+017 are found to be well below previous estimates of up to 1e12 g/s, and likely below 1e10 g/s, thus suggesting the star-disk system may be average in its evolutionary state, and only special in viewing geometry.Comment: 14 pages, 7 figures, 3 tables; accepted to MNRA

    Spectrum of surface-mode contributions to the excitation probability for electron beam interacting with sharp-edged dielectric wedges

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    The interaction of a nonrelativistic charged particle beam, travelling parallel to the surface of a sharp-edged dielectric wedge is analyzed. The general expressions for excitation probability are obtained for a beam moving along the direction of a symmetry axis, either outside or inside the dielectric wedge. The dielectric function of the medium is assumed to be isotropic, and numerical results are given for the materials of experimental interest.Comment: LaTeX 2.09, 15 pages, 10 figure

    Combination Rules, Charge Symmetry, and Hall Effect in Cuprates

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    The rule relating the observed Hall coefficient to the spin and charge responses of the uniform doped Mott insulator is derived. It is essential to include the contribution of holon and spinon three-current correlations to the effective action of the gauge field. In the vicinity of the Mott insulating point the Hall coefficient is holon dominated and weakly temperature dependent. In the vicinity of a point of charge conjugation symmetry the holon contribution to the observed Hall coefficient is small: the Hall coefficient follows the temperature dependence of the diamagnetic susceptibility with a sign determined by the Fermi surface shape. NOTE: document prepared using REVTEX. (3 Figs, not included, available on request from: [email protected])Comment: 8 page

    SuperWASP: Wide Angle Search for Planets

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    SuperWASP is a fully robotic, ultra-wide angle survey for planetary transits. Currently under construction, it will consist of 5 cameras, each monitoring a 9.5 x 9.5 deg field of view. The Torus mount and enclosure will be fully automated and linked to a built-in weather station. We aim to begin observations at the beginning of 2003.Comment: 4 pages, 1 figure, to be published in proceedings of "Scientific Frontiers in Research on Extrasolar Planets

    LRG-BEASTS: Evidence for clouds in the transmission spectrum of HATS-46 b

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    We have performed low-resolution ground-based spectroscopy of HATS-46 b in transmission, using the EFOSC2 instrument on the ESO New Technology Telescope (NTT). HATS-46 b is a highly-inflated exoplanet that is a prime target for transmission spectroscopy, having a Jupiter-like radius (0.95 RJup_\textrm{Jup}) but a much lower mass (0.16 MJup_\textrm{Jup}). It orbits a G-type star with a 4.7 d period, giving an equilibrium temperature of 1100 K. We observed one transit of HATS-46 b with the NTT, with the time-series spectra covering a wavelength range of 3900 - 9000 Angstrom at a resolution of R∼380R \sim 380. We achieved a remarkably precise transmission spectrum of 1.03 ×\times photon noise, with a median uncertainty of 357357 ppm for ∼200\sim 200 Angstrom wide bins, despite the relative faintness of the host star with Vmag=13.6V_{\mathrm{mag}} = 13.6. The transmission spectrum does not show strong absorption features and retrievals favour a cloudy model, ruling out a clear atmosphere with 3.0σ3.0\sigma confidence. We also place a conservative upper limit on the sodium abundance under the alternative scenario of a clear atmosphere. This is the eighth planet in the LRG-BEASTS survey, which uses 4m-class telescopes such as the NTT to obtain low-resolution transmission spectra of hot Jupiters with precisions of around one atmospheric scale height.Comment: 10 pages, 7 figures, 4 tables, accepted for publication in MNRA
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