Use of tunable nanopore blockade rates to investigate colloidal dispersions

Tunable nanopores in elastomeric membranes have been used to study the dependence of ionic current blockade rate on the concentration and electrophoretic mobility of particles in aqueous suspensions. A range of nanoparticle sizes, materials and surface functionalities has been tested. Using pressure-driven flow through a pore, the blockade rate for 100 nm carboxylated polystyrene particles was found to be linearly proportional to both transmembrane pressure (controlled between 0 and 1.8 kPa) and particle concentration (between 7 x 10^8 and 4.5 x 10^10 mL^-1). This result can be accurately modelled using Nernst-Planck transport theory. Using only an applied potential across a pore, the blockade rates for carboxylic acid and amine coated 500 nm and 200 nm silica particles were found to correspond to changes in their mobility as a function of the solution pH. Scanning electron microscopy and confocal microscopy have been used to visualise changes in the tunable nanopore geometry in three dimensions as a function of applied mechanical strain. The pores observed were conical in shape, and changes in pore size were consistent with ionic current measurements. A zone of inelastic deformation adjacent to the pore has been identified as critical in the tuning process

Energy balance simulation of a wheat canopy using the RZ-SHAW (RZWQM-SHAW) model

RZ-SHAW is a new hybrid model coupling the Root Zone Water Quality Model (RZWQM) and the Simultaneous Heat and Water (SHAW) model to extend RZWQM applications to conditions of frozen soil and crop residue cover. RZ-SHAW offers the comprehensive land management options of RZWQM with the additional capability to simulate diurnal changes in energy balance needed for simulating the near-surface microclimate and leaf temperature. The objective of this study was to evaluate RZ-SHAW for simulations of radiation balance and sensible and latent heat fluxes over plant canopies. Canopy energy balance data were collected at various growing stages of winter wheat in the North China Plain (36° 57'N, 116° 6'E, 28 m above sea level). RZ-SHAW and SHAW simulations using hourly meteorological data were compared with measured net radiation, latent heat flux, sensible heat flux, and soil heat flux. RZ-SHAW provided similar goodness-of-prediction statistics as the original SHAW model for all the energy balance components when using observed plant growth input data. The root mean square error (RMSE) for simulated net radiation, latent heat, sensible heat, and soil heat fluxes was 29.7, 30.7, 29.9, and 25.9 W m -2 for SHAW and 30.6, 32.9, 34.2, and 30.6 W m -2 for RZ-SHAW, respectively. Nash-Sutcliffe R 2 ranged from 0.67 for sensible heat flux to 0.98 for net radiation. Subsequently, an analysis was performed using the plant growth component of RZ-SHAW instead of inputting LAI and plant height. The model simulation results agreed with measured plant height, yield, and LAI very well. As a result, RMSE for the energy balance components were very similar to the original RZ-SHAW simulation, and latent, sensible, and soil heat fluxes were actually simulated slightly better. RMSE for simulated net radiation, latent heat, sensible heat, and soil heat fluxes was 31.5, 30.4, 30.2, and 27.6 W m -2, respectively. Overall, the results demonstrated a successful coupling of RZWQM and SHAW in terms of canopy energy balance simulation, which has important implications for prediction of crop growth, crop water stress, and irrigation scheduling

A Translation Initiation Element Specific to mRNAs with Very Short 5′UTR that Also Regulates Transcription

Transcription is controlled by cis regulatory elements, which if localized downstream to the transcriptional start site (TSS), in the 5′UTR, could influence translation as well. However presently there is little evidence for such composite regulatory elements. We have identified by computational analysis an abundant element located downstream to the TSS up to position +30, which controls both transcription and translation. This element has an invariable ATG sequence, which serves as the translation initiation codon in 64% of the genes bearing it. In these genes the initiating AUG is preceded by an extremely short 5′UTR. We show that translation in vitro and in vivo is initiated exclusively from the AUG of this motif, and that the AUG flanking sequences create a strong translation initiation context. This motif is distinguished from the well-known Kozak in its unique ability to direct efficient and accurate translation initiation from mRNAs with a very short 5′UTR. We therefore named it TISU for Translation Initiator of Short 5′UTR. Interestingly, this translation initiation element is also an essential transcription regulatory element of Yin Yang 1. Our characterization of a common transcription and translation element points to a link between mammalian transcription and translation initiation

Temperature changes over storms from measurements of spacecraft TIMED

In the present work we have studied changes of mesospheric temperature over the powerful storms Wilma, Haitang, and Katrina using measurements of the space vehicle TIMED. We have found the temperature increasing at the altitude range 80-100 km. We have found the explanations for the obtained results by the dissipation of the gravity waves. Propagation of atmospheric gravity waves in a non-isothermal, windless atmosphere, with taking into account the viscosity and the thermal conductivity, has also been modelled in this work. We have determined that the maximum of amplitude of the atmospheric-gravity waves at the considered characteristics corresponds to altitudes of near 90 km (mesopause). It was found that the main factor influencing propagation and dissipation of the wave in such cases is the vertical temperature gradient. Viscosity and thermal conductivity have less influence on the wave amplitude

Design of a pulse power supply unit for micro-ECM

Electrochemical micro-machining (μECM) requires a particular pulse power supply unit (PSU) to be developed in order to achieve desired machining performance. This paper summarises the development of a pulse PSU meeting the requirements of μECM. The pulse power supply provides tens of nanosecond pulse duration, positive and negative bias voltages and a polarity switching functionality. It fulfils the needs for tool preparation with reversed pulsed ECM on the machine. Moreover, the PSU is equipped with an ultrafast overcurrent protection which prevents the tool electrode from being damaged in case of short circuits. The developed pulse PSU was used to fabricate micro-tools out of 170 μm WC-Co alloy shafts via micro-electrochemical turning and drill deep holes via μECM in a disk made of 18NiCr6. The electrolyte used for both processes was a mixture of sulphuric acid and NaNO3 aqueous solutions.The research reported in this paper is supported by the European Commission within the project “Minimizing Defects in Micro-Manufacturing Applications (MIDEMMA)” (FP7-2011-NMP-ICT-FoF-285614

The +4G Site in Kozak Consensus Is Not Related to the Efficiency of Translation Initiation

The optimal context for translation initiation in mammalian species is GCCRCCaugG (where R = purine and “aug” is the initiation codon), with the -3R and +4G being particularly important. The presence of +4G has been interpreted as necessary for efficient translation initiation. Accumulated experimental and bioinformatic evidence has suggested an alternative explanation based on amino acid constraint on the second codon, i.e., amino acid Ala or Gly are needed as the second amino acid in the nascent peptide for the cleavage of the initiator Met, and the consequent overuse of Ala and Gly codons (GCN and GGN) leads to the +4G consensus. I performed a critical test of these alternative hypotheses on +4G based on 34169 human protein-coding genes and published gene expression data. The result shows that the prevalence of +4G is not related to translation initiation. Among the five G-starting codons, only alanine codons (GCN), and glycine codons (GGN) to a much smaller extent, are overrepresented at the second codon, whereas the other three codons are not overrepresented. While highly expressed genes have more +4G than lowly expressed genes, the difference is caused by GCN and GGN codons at the second codon. These results are inconsistent with +4G being needed for efficient translation initiation, but consistent with the proposal of amino acid constraint hypothesis

Investigation of sandwich material surface created by abrasive water jet (AWJ) via vibration emission

The paper presents research a of abrasive waterjet cutting of heterogeneous “sandwich“ material with different Young modulus of elasticity of the cutted surface geometry by means of vibration emission. In order to confirm hypothetical assumptions about direct relation between vibration emission and surface quality an experiment in heterogeneous material consisting of stainless steel (DIN 1.4006 / AISI 410) and alloy AlCuMg2 has been provided

Synthesis of two SAPAP3 isoforms from a single mRNA is mediated via alternative translational initiation

In mammalian neurons, targeting and translation of specific mRNAs in dendrites contribute to synaptic plasticity. After nuclear export, mRNAs designated for dendritic transport are generally assumed to be translationally dormant and activity of individual synapses may locally trigger their extrasomatic translation. We show that the long, GC-rich 5′-untranslated region of dendritic SAPAP3 mRNA restricts translation initiation via a mechanism that involves an upstream open reading frame (uORF). In addition, the uORF enables the use of an alternative translation start site, permitting synthesis of two SAPAP3 isoforms from a single mRNA. While both isoforms progressively accumulate at postsynaptic densities during early rat brain development, their levels relative to each other vary in different adult rat brain areas. Thus, alternative translation initiation events appear to regulate relative expression of distinct SAPAP3 isoforms in different brain regions, which may function to influence synaptic plasticity