Experimental study of a generic high-speed civil transport

An experimental study of generic high-speed civil transport was conducted in the NASA Langley 8-ft Transonic Pressure Tunnel. The data base was obtained for the purpose of assessing the accuracy of various levels of computational analysis. Two models differing only in wingtip geometry were tested with and without flow-through nacelles. The baseline model has a curved or crescent wingtip shape, while the second model has a more conventional straight wingtip shape. The study was conducted at Mach numbers from 0.30 to 1.19. Force data were obtained on both the straight wingtip model and the curved wingtip model. Only the curved wingtip model was instrumented for measuring pressures. Selected longitudinal, lateral, and directional data are presented for both models. Selected pressure distributions for the curved wingtip model are also presented

Investigation of a 2-Colour Undulator FEL Using Puffin

Initial studies of a 2-colour FEL amplifier using one monoenergetic electron beam are presented. The interaction is modelled using the unaveraged, broadband FEL code Puffin. A series of undulator modules are tuned to generate two resonant frequencies along the FEL interaction and a self-consistent 2-colour FEL interaction at widely spaced non-harmonic wavelengths at 1nm and 2.4nm is demonstrated.Comment: Submitted to The 35th International Free-Electron Laser Conference, Manhattan, New York (2013

The credibility of health economic models for health policy decision-making: the case of population screening for abdominal aortic aneurysm

<i>Objectives</i>: To review health economic models of population screening for abdominal aortic aneurysm (AAA) among elderly males and assess their credibility for informing decision-making. <i>Methods</i>: A literature review identified health economic models of ultrasound screening for AAA. For each model focussing on population screening in elderly males, model structure and input parameter values were critically appraised using published good practice guidelines for decision analytic models. <i>Results</i>: Twelve models published between 1989 and 2003 were identified. Converting costs to a common currency and base year, substantial variability in cost-effectiveness results were revealed. Appraisals carried out for the nine models focusing on population screening showed differences in their complexity, with the simpler models generating results most favourable to screening. Eight of the nine models incorporated two or more simplifying structural assumptions favouring screening; uncertainty surrounding these assumptions was not investigated by any model. Quality assessments on a small number of parameters revealed input values varied between models, methods used to identify and incorporate input data were often not described, and few sensitivity analyses were reported. <i>Conclusions</i>: Large variation exists in the cost-effectiveness results generated by AAA screening models. The substantial number of factors potentially contributing to such disparities means that reconciliation of model results is impossible. In addition, poor reporting of methods makes it difficult to identify the most plausible and thus most useful model of those developed

Revisiting the 1954 Suspension Experiments of R. A.Bagnold

In 1954 R. A. Bagnold published his seminal findings on the rheological properties of a liquid-solid suspension. Although this work has been cited extensively over the last fifty years, there has not been a critical review of the experiments. The purpose of this study is to examine the work and to suggest an alternative reason for the experimental findings. The concentric cylinder rheometer was designed to measure simultaneously the shear and normal forces for a wide range of solid concentrations, fluid viscosities and shear rates. As presented by Bagnold, the analysis and experiments demonstrated that the shear and normal forces depended linearly on the shear rate in the 'macroviscous' regime; as the grain-to-grain interactions increased in the 'grain-inertia' regime, the stresses depended on the square of the shear rate and were independent of the fluid viscosity. These results, however, appear to be dictated by the design of the experimental facility. In Bagnold's experiments, the height (h) of the rheometer was relatively short compared to the spacing (t) between the rotating outer and stationary inner cylinder (h/t=4.6). Since the top and bottom end plates rotated with the outer cylinder, the flow contained two axisymmetric counter-rotating cells in which flow moved outward along the end plates and inward through the central region of the annulus. At higher Reynolds numbers, these cells contributed significantly to the measured torque, as demonstrated by comparing Bagnold's pure-fluid measurements with studies on laminar-to-turbulent transitions that pre-date the 1954 study. By accounting for the torque along the end walls, Bagnold's shear stress measurements can be estimated by modelling the liquid-solid mixture as a Newtonian fluid with a corrected viscosity that depends on the solids concentration. An analysis of the normal stress measurements was problematic because the gross measurements were not reported and could not be obtained

Species sensitivity of zeolite minerals for uptake of mercury solutes

The uptake of inorganic Hg2+ and organometallic CH3Hg+ from aqueous solutions by 11 different natural zeolites has been investigated using a batch distribution coefficient (Kd) method and supported by a preliminary voltammetric study. The effect of mercury concentration on theKd response is shown over an environmentally appropriate concentration range of 0.1-5 ppm inorganic and organometallic Hg using a batch factor of 100 ml g−1 and 20 h equilibration. Analcime and a Na-chabazite displayed the greatest methylmercury uptakes (Kd values at 1.5 ppm of 4023 and 3456, respectively), with mordenite as the smallest at 578. All uptake responses were greater for methylmercury than for the inorganic mercuric nitrate solutions, suggesting a distinctive sensitivity of zeolites to reaction with different types of solute species. It is likely that this sensitivity is attributable to the precise nature of the resultant Hg-zeolite bonds. Additionally, both the Si-Al ratio and the Na content of the initial natural zeolite samples are shown to influence the Kd responses, with positive correlations between Kd and Na content for all zeolites excluding mordenite

Dynamic removal of replication protein A by Dna2 facilitates primer cleavage during Okazaki fragment processing in Saccharomyces cerevisiae

Eukaryotic Okazaki fragments are initiated by an RNA/DNA primer, which is removed before the fragments are joined. Polymerase d displaces the primer into a flap for processing. Dna2 nuclease/helicase and flap endonuclease 1 (FEN1) are proposed to cleave the flap. The single-stranded DNA binding protein, replication protein A (RPA), governs cleavage activity. Flap-bound RPA inhibits FEN1. This necessitates cleavage by Dna2, which is stimulated by RPA. FEN1 then cuts the remaining RPA-free flap to create a nick for ligation. Cleavage by Dna2 requires that it enter the 5'-end and track down the flap. Since Dna2 cleaves the RPA-bound flap, we investigated the mechanism by which Dna2 accesses the protein-coated flap for cleavage. Using a nuclease-defective Dna2 mutant, we showed that just binding of Dna2 dissociates the flap-bound RPA. Facile dissociation is specific to substrates with a genuine flap, and will not occur with an RPA-coated single strand. We also compared the cleavage patterns of Dna2 with and without RPA to better define RPA stimulation of Dna2. Stimulation derived from removal of DNA folding in the flap. Apparently, coordinated with its dissociation, RPA relinquishes the flap to Dna2 for tracking in a way that does not allow flap structure to reform. We also found that RPA strand melting activity promotes excessive flap elongation, but it is suppressed by Dna2-promoted RPA dissociation. Overall, results indicate that Dna2 and RPA coordinate their functions for efficient flap cleavage and preparation for FEN1

A real-time PCR method for quantification of the total and major variant strains of the Deformed wing virus

Funding: ELB was supported by a Biotechnology and Biological Sciences Research Council (BBSRC) EASTBIO Doctoral Training Partnership (http://www.bbsrc.ac.uk) [grant number BB/J01446X/1] and an Eastern Association Regional Studentship (EARS) and The Morley Agricultural Foundation awarded to ASB. CRC was supported by a KTN BBSRC CASE studentship (BB/M503526/1) (http://www.bbsrc.ac.uk), part-funded by the Scottish Beekeeping Association (https://www.scottishbeekeepers.org.uk/) and the Animal Health - Disease Prevention, Scottish Government awarded to ASB CRC. This project received funding from the European Union’s Seventh Framework Programme for research, technological development and demonstration under grant agreement no 613960 (SMARTBEES) (http://www.smartbees-fp7.eu/) awarded to ASB. The funders had no role in study design, data collection and analysis decision to publish, or preparation of the manuscript. Acknowledgments The authors wish to thank Mr W. Thrale, Mr Z. Blackmore, Mr J. Quinlan, and Mr J. Palombo for sample collection from the South East of England and Margie Ramsey for Beinn Eighe National Nature Reserve sample collection.Peer reviewedPublisher PD

Questions of quality in repositories of open educational resources: a literature review

Open educational resources (OER) are teaching and learning materials which are freely available and openly licensed. Repositories of OER (ROER) are platforms that host and facilitate access to these resources. ROER should not just be designed to store this content – in keeping with the aims of the OER movement, they should support educators in embracing open educational practices (OEP) such as searching for and retrieving content that they will reuse, adapt or modify as needed, without economic barriers or copyright restrictions. This paper reviews key literature on OER and ROER, in order to understand the roles ROER are said or supposed to fulfil in relation to furthering the aims of the OER movement. Four themes which should shape repository design are identified, and the following 10 quality indicators (QI) for ROER effectiveness are discussed: featured resources; user evaluation tools; peer review; authorship of the resources; keywords of the resources; use of standardised metadata; multilingualism of the repositories; inclusion of social media tools; specification of the creative commons license; availability of the source code or original files. These QI form the basis of a method for the evaluation of ROER initiatives which, in concert with considerations of achievability and long-term sustainability, should assist in enhancement and development. Keywords: open educational resources; open access; open educational practice; repositories; quality assuranc