Collaborative Learning Technologies in Teaching Preschool Twins

This paper focuses on applying collaborative learning technologies in teaching preschool twins and reveals the role of collaborative learning in the formation of twins’ self-awareness. At the ascertaining stage of the experiment, 100 twins of preschool age took part. At the formative stage of the experiment, 16 preschoolers took part. At the formative stage of the experiment, an empirical assessment was carried out based on empirical indicators of the process and result of joint task performance by twins before and after participation in the formative experiment. An empirical study of the peculiarities of intrapair interaction in collaborative activities of preschool twins has shown effects that are a consequence of the twin situation. The use of collaborative learning in the psychological and pedagogical support of preschool twins has allowed obtaining positive results expressed in an increase in self-esteem, motivation for cooperation, and a decrease in aggressiveness and rivalry in a pair of twins

The role of legal consciousness in the implementation of preparedness for the dangerous driving of road drivers

The article discusses the role of legal consciousness in enhancing the internal factors of preparedness for dangerous driving. The analysis of statistical data regarding traffic accidents is given. The external and internal predictors of dangerous driving are analyzed. The results of an empirical study showing the relationship of the style of driving a vehicle and the development of the legal consciousness of road users are discussed

The Impact of the Global Financial Crisis on Russia's Cooperation with the Countries of EurAsEC

The article considers the impact of the financial and economic crisis on Russia's cooperation with the EurAsEC countries. Because crises are an integral part of any economic system, a deep analysis should be carried out. We consider the integration at the micro level and prospects of Russia's cooperation with the EurAsEC, the prospects for cooperation between Russia and the EurAsEC countries should be assessed in different ways. Speaking about the cooperation it should be noted that the main weakness of the countries dependent on the prices of natural resources, which are the main export of all countries, including Russia. After all, the raw material orientation determines the country's place in the world. And so the main task here is to develop the production of finished products and the development of knowledge-based industries. Keywords: EurAsEC countries, financial crisis, problems of cooperation, economic globalization. JEL Classifications: F63, F65, G01, H1

IgG-like bispecific antibodies with potent and synergistic neutralization against circulating SARS-CoV-2 variants of concern

Monoclonal antibodies are a promising approach to treat COVID-19, however the emergence of SARS-CoV-2 variants has challenged the efficacy and future of these therapies. Antibody cocktails are being employed to mitigate these challenges, but neutralization escape remains a major challenge and alternative strategies are needed. Here we present two anti-SARS-CoV-2 spike binding antibodies, one Class 1 and one Class 4, selected from our non-immune human single-chain variable fragment (scFv) phage library, that are engineered into four, fully-human IgG-like bispecific antibodies (BsAb). Prophylaxis of hACE2 mice and post-infection treatment of golden hamsters demonstrates the efficacy of the monospecific antibodies against the original Wuhan strain, while promising in vitro results with the BsAbs demonstrate enhanced binding and distinct synergistic effects on neutralizing activity against circulating variants of concern. In particular, one BsAb engineered in a tandem scFv-Fc configuration shows synergistic neutralization activity against several variants of concern including B.1.617.2. This work provides evidence that synergistic neutralization can be achieved using a BsAb scaffold, and serves as a foundation for the future development of broadly reactive BsAbs against emerging variants of concern

The algal composition and structure of the Yaitskoe lake (Samara Region, Russia)

We studied the phytoplankton of the Yaitskoe Lake (Samara region, Russia) from May to October 2009–2011. In this water body, there were 312 species and intraspecific taxa of algae. They belonged to nine taxonomic Divisions, 14 Classes, 22 Orders, 52 Families, and 108 Genera. A larger number of species, varieties and forms of algae belonged to the Chlorophyta, which included 37% of the total number of species and intraspecific taxa, followed by Bacillariophyta (21%), Euglenophyta (14%), and Cyanoprokaryota (12%). The other algae species number was less than 10% of the total species richness. The value of algae quantitative development was high in the Yaitskoe Lake during the three-year period. The values of abundance and biomass increased from spring to the end of summer, then there was a decrease. The level of trophic state was assessed as eutrophic. The abundance and biomass of algae were high during the study. The level of species diversity and evenness in phytoplankton community were assessed as high. Indices values, calculated by the phytoplankton abundance, were maximal at late spring. In summer, their values decreased. This was due to the increased dominance of blue-green algae (cyanoprokaryotes). The degree of cyanoprokaryota dominance decreased in autumn, while species diversity and evenness of the community increased. The indices, calculated on biomass, decreased from spring to early autumn. Their minimal values were during the «blooming» of water, and then gradually increased. Saprobiological analysis showed that this pond belonged to the β-mesosaprobic type, moderately polluted with Class III of water quality

Lanthanide 9-anthracenate: solution processable emitters for efficient purely NIR emitting host-free OLEDs

Searching for new NIR emitting materials, lanthanide 9-anthracenates Ln(ant)3 were synthesized and thoroughly characterized. Ytterbium 9-anthracenate Yb(ant)3, that demonstrated the highest NIR luminescence efficiency, was successfully used as an emission layer of a host-free OLED and its electroluminescence quantum efficiency, corresponding to the sole band at 1000 nm, reached 0.21%. This performance could be achieved due to the high quantum yield of Yb(ant)3, which reached 1.5% and was increased up to 2.5% by partial Yb3+ substitution with Lu3+, as well as its high electron mobility due to the extended stacking in its crystal structure. The first gadolinium-based PHOLED was prepared based on Gd(ant)3

Comparative Study of Cytotoxic and Membranotropic Properties of Betulinic Acid-F16 Conjugate on Breast Adenocarcinoma Cells (MCF-7) and Primary Human Fibroblasts

The present study evaluates the cytotoxicity of a previously synthesized conjugate of betulinic acid (BA) with the penetrating cation F16 on breast adenocarcinoma (MCF-7) and human fibroblast (HF) cell lines, and also shows the mechanism underlying its membranotropic action. It was confirmed that the conjugate exhibits higher cytotoxicity compared to native BA at low doses also blocking the proliferation of both cell lines and causing cell cycle arrest in the G0/G1 phase. We show that the conjugate indeed has a high potential for accumulation in mitochondria, being visualized in these organelles, which is most pronounced in cancer cells. The effect of the conjugate was observed to be accompanied by ROS hyperproduction in both cancerous and healthy cells, despite the lower base level of ROS in the latter. Along with this, using artificial liposomes, we determined that the conjugate is able to influence the phase state of lipid membranes, make them more fluid, and induce nonspecific permeabilization contributing to the overall cytotoxicity of the tested agent. We conclude that the studied BA–F16 conjugate does not have significant selective cytotoxicity, at least against the studied breast cancer cell line MCF-7