Evaluation of Trace Mineral Sources on Newly Arrived Stocker Cattle

Light weight stocker calves experience variable degrees of physiological and psychological stressors as they are assembled from various marketing points and transported to their destination. Susceptibility to disease in young, long hauled calves is greatly enhanced and the consequence of sickness is a major cause for poor production outcomes. Enhanced nutritional trace mineral programs that contain zinc, copper, and manganese from organic or hydroxy sources may be more efficacious as a means of minimizing disease or realizing improved performance than the sulfate form of these respective trace minerals

Feeding a Novel Trace Mineral at Lower Levels to Grazing Stocker Cattle Does Not Impair Performance

When grazing stocker cattle on native Flint Hills pasture, optimizing growth rate is important in determining overall profitability. The correct selection of mineral supplements is an important decision that can be used to help promote overall productivity during a grazing season

Syngenta Enogen Feed Corn Containing an Alpha Amylase Expression Trait Improves Digestibility in Growing Calf Diets

Objective: To evaluate the digestibility parameters of growing cattle when fed Enogen Feed corn. Study Description: Seven cannulated Holstein steers were used to determine the effects on digestibility when fed Enogen Feed corn (Syngenta) as whole-corn or processed as dry-rolled at ad libitum intake. The Bottom Line: When Enogen Feed corn was fed in an ad libitum fashion to growing calves, dry matter and organic matter are digested to a greater extent relative to yellow corn

Quality of Data Entry Using Single Entry, Double Entry and Automated Forms Processing–An Example Based on a Study of Patient-Reported Outcomes

Background: The clinical and scientific usage of patient-reported outcome measures is increasing in the health services. Often paper forms are used. Manual double entry of data is defined as the definitive gold standard for transferring data to an electronic format, but the process is laborious. Automated forms processing may be an alternative, but further validation is warranted. Methods: 200 patients were randomly selected from a cohort of 5777 patients who had previously answered two different questionnaires. The questionnaires were scanned using an automated forms processing technique, as well as processed by single and double manual data entry, using the EpiData Entry data entry program. The main outcome measure was the proportion of correctly entered numbers at question, form and study level. Results: Manual double-key data entry (error proportion per 1000 fields = 0.046 (95 % CI: 0.001–0.258)) performed better than single-key data entry (error proportion per 1000 fields = 0.370 (95 % CI: 0.160–0.729), (p = 0.020)). There was no statistical difference between Optical Mark Recognition (error proportion per 1000 fields = 0.046 (95 % CI: 0.001–0.258)) and double-key data entry (p = 1.000). With the Intelligent Character Recognition method, there was no statistical difference compared to single-key data entry (error proportion per 1000 fields = 6.734 (95 % CI: 0.817–24.113), (p = 0.656)), as well as double-key data entry (error proportion per 1000 fields = 3.367 (95 % CI: 0.085–18.616)), (p = 0.319))

Neue Systeme zur Antikörperherstellung

Antibodies are indispensable tools for therapy and diagnostics. Here, we present a novel technique that allows for an accelerated synthesis of functionally active antibodies. This is achieved by using a cell-free translation system based on mammalian cells

Functional analysis of membrane proteins produced by cell-free translation

Cell-free production is a valuable and alternative method for the synthesis of membrane proteins. This system offers openness allowing the researchers to modify the reaction conditions without any boundaries. Additionally, the cell-free reactions are scalable from 20 μL up to several mL, faster and suitable for the high-throughput protein production. Here, we present two cell-free systems derived from Escherichia coli (E. coli) and Spodoptera frugiperda (Sf21) lysates. In the case of the E. coli cell-free system, nanodiscs are used for the solubilization and purification of membrane proteins. In the case of the Sf21 system, endogenous microsomes with an active translocon complex are present within the lysates which facilitate the incorporation of the bacterial potassium channel KcsA within the microsomal membranes. Following cell-free synthesis, these microsomes are directly used for the functional analysis of membrane proteins