Non-linear propagation of kink waves to the solar chromosphere

Small-scale magnetic field concentrations (magnetic elements) in the quiet Sun are believed to contribute to the energy budget of the upper layers of the Sun's atmosphere, as they are observed to support a large number of MHD modes. In recent years, kink waves in magnetic elements were observed at different heights in the solar atmosphere, from the photosphere to the corona. However, the propagation of these waves has not been fully evaluated. Our aim is to investigate the propagation of kink waves in small magnetic elements in the solar atmosphere. We analysed spectropolarimetric data of high-quality and long duration of a photospheric quiet Sun region observed near the disk center with the spectropolarimeter CRISP at the Swedish Solar Telescope (SST), and complemented by simultaneous and co-spatial broad-band chromospheric observations of the same region. Our findings reveal a clear upward propagation of kink waves with frequency above $~2.6$ mHz. Moreover, the signature of a non-linear propagation process is also observed. By comparing photospheric to chromospheric power spectra, no signature of an energy dissipation is found at least at the atmospheric heights at which the data analysed originate. This implies that most of the energy carried by the kink waves (within the frequency range under study $< 17$ mHz) flows to upper layers in the Sun's atmosphere.Comment: A&A accepte

High-frequency Oscillations in Small Magnetic Elements Observed with Sunrise/SuFI

We characterize waves in small magnetic elements and investigate their propagation in the lower solar atmosphere from observations at high spatial and temporal resolution. We use the wavelet transform to analyze oscillations of both horizontal displacement and intensity in magnetic bright points found in the 300 nm and the Ca II H 396.8 nm passbands of the filter imager on board the Sunrise balloon-borne solar observatory. Phase differences between the oscillations at the two atmospheric layers corresponding to the two passbands reveal upward propagating waves at high frequencies (up to 30 mHz). Weak signatures of standing as well as downward propagating waves are also obtained. Both compressible and incompressible (kink) waves are found in the small-scale magnetic features. The two types of waves have different, though overlapping, period distributions. Two independent estimates give a height difference of approximately 450+-100 km between the two atmospheric layers sampled by the employed spectral bands. This value, together with the determined short travel times of the transverse and longitudinal waves provide us with phase speeds of 29+-2 km/s and 31+-2 km/s, respectively. We speculate that these phase speeds may not reflect the true propagation speeds of the waves. Thus, effects such as the refraction of fast longitudinal waves may contribute to an overestimate of the phase speed.Comment: 14 pages, 7 figure

Migration of Ca II H bright points in the internetwork

The migration of magnetic bright point-like features (MBP) in the lower solar atmosphere reflects the dispersal of magnetic flux as well as the horizontal flows of the atmospheric layer they are embedded in. We analyse trajectories of the proper motion of intrinsically magnetic, isolated internetwork Ca II H MBPs (mean lifetime 461 +- 9 s) to obtain their diffusivity behaviour. We use seeing-free high spatial and temporal resolution image sequences of quiet-Sun, disc-centre observations obtained in the Ca II H 3968 {\AA} passband of the Sunrise Filter Imager (SuFI) onboard the Sunrise balloon-borne solar observatory. Small MBPs in the internetwork are automatically tracked. The trajectory of each MBP is then calculated and described by a diffusion index ({\gamma}) and a diffusion coefficient (D). We further explore the distribution of the diffusion indices with the help of a Monte Carlo simulation. We find {\gamma} = 1.69 +- 0.08 and D = 257 +- 32 km^2/s averaged over all MBPs. Trajectories of most MBPs are classified as super-diffusive, i.e., {\gamma} > 1, with the determined {\gamma} being to our knowledge the largest obtained so far. A direct correlation between D and time-scale ({\tau}) determined from trajectories of all MBPs is also obtained. We discuss a simple scenario to explain the diffusivity of the observed, relatively short-lived MBPs while they migrate within a small area in a supergranule (i.e., an internetwork area). We show that the scatter in the {\gamma} values obtained for individual MBPs is due to their limited lifetimes. The super-diffusive MBPs can be well-described as random walkers (due to granular evolution and intergranular turbu- lence) superposed on a large systematic (background) velocity, caused by granular, mesogranular and supergranular flows.Comment: 10 pages, 7 figures, 3 table

Inclinations of small quiet-Sun magnetic features based on a new geometric approach

High levels of horizontal magnetic flux have been reported in the quiet-Sun internetwork, often based on Stokes profile inversions. Here we introduce a new method for deducing the inclination of magnetic elements and use it to test magnetic field inclinations from inversions. We determine accurate positions of a set of small, bright magnetic elements in high spatial resolution images sampling different photospheric heights obtained by the Sunrise balloon-borne solar observatory. Together with estimates of the formation heights of the employed spectral bands, these provide us with the inclinations of the magnetic features. We also compute the magnetic inclination angle of the same magnetic features from the inversion of simultaneously recorded Stokes parameters. Our new, geometric method returns nearly vertical fields (average inclination of around 14 deg with a relatively narrow distribution having a standard deviation of 6 deg). In strong contrast to this, the traditionally used inversions give almost horizontal fields (average inclination of 75+-8 deg) for the same small magnetic features, whose linearly polarised Stokes profiles are adversely affected by noise. The almost vertical field of bright magnetic features from our geometric method is clearly incompatible with the nearly horizontal magnetic fields obtained from the inversions. This indicates that the amount of magnetic flux in horizontal fields deduced from inversions is overestimated in the presence of weak Stokes signals, in particular if Stokes Q and U are close to or under the noise level. By combining the proposed method with inversions we are not just improving the inclination, but also the field strength. This technique allows us to analyse features that are not reliably treated by inversions, thus greatly extending our capability to study the complete magnetic field of the quiet Sun.Comment: 12 pages, 9 figures, 1 table; Accepted for publication in Astronomy & Astrophysic

Establishment and characterization of two human breast carcinoma cell lines by spontaneous immortalization: Discordance between Estrogen, Progesterone and HER2/neu receptors of breast carcinoma tissues with derived cell lines

Background: Breast cancer is one of the most common cancers among women throughout the world. Therefore, established cell lines are widely used as in vitro experimental models in cancer research.Methods: Two continuous human breast cell lines, designated MBC1 and MBC2, were successfully established and characterized from invasive ductal breast carcinoma tissues of Malaysian patients. MBC1 and MBC2 have been characterized in terms of morphology analysis, population doubling time, clonogenic formation, wound healing assay, invasion assay, cell cycle, DNA profiling, fluorescence immunocytochemistry, Western blotting and karyotyping.Results: MBC1 and MBC2 exhibited adherent monolayer epithelial morphology at a passage number of 150. Receptor status of MBC1 and MBC2 show (ER+, PR+, HER2+) and (ER+, PR-, HER2+), respectively. These results are in discordance with histopathological studies of the tumoral tissues, which were triple negative and (ER-, PR-, HER2+) for MBC1 and MBC2, respectively. Both cell lines were capable of growing in soft agar culture, which suggests their metastatic potential. The MBC1 and MBC2 metaphase spreads showed an abnormal karyotype, including hyperdiploidy and complex rearrangements with modes of 52-58 chromosomes per cell.Conclusions: Loss or gain in secondary properties, deregulation and specific genetic changes possibly conferred receptor changes during the culturing of tumoral cells. Thus, we hypothesize that, among heterogenous tumoral cells, only a small minority of ER+/PR+/HER2+ and ER+/PR-/HER2+ cells with lower energy metabolism might survive and adjust easily to in vitro conditions. These cell lines will pave the way for new perspectives in genetic and biological investigations, drug resistance and chemotherapy studies, and would serve as prototype models in Malaysian breast carcinogenesis investigations. © 2012 Kamalidehghan et al.; licensee BioMed Central Ltd

Development of 3D PCL microsphere/TiO\u3csub\u3e2\u3c/sub\u3e nanotube composite scaffolds for bone tissue engineering

In this research, the three dimensional porous scaffolds made of a polycaprolactone (PCL) microsphere/TiO2 nanotube (TNT) composite was fabricated and evaluated for potential bone substitute applications. We used a microsphere sintering method to produce three dimensional PCL microsphere/TNT composite scaffolds. The mechanical properties of composite scaffolds were regulated by varying parameters, such as sintering time, microsphere diameter range size and PCL/TNT ratio. The obtained results ascertained that the PCL/TNT (0.5 wt%) scaffold sintered at 60 °C for 90 min had the most optimal mechanical properties and an appropriate pore structure for bone tissue engineering applications. The average pore size and total porosity percentage increased after increasing the microsphere diameter range for PCL and PCL/TNT (0.5 wt%) scaffolds. The degradation rate was relatively high in PCL/TNT (0.5 wt%) composites compared to pure PCL when the samples were placed in the simulated body fluid (SBF) for 6 weeks. Also, the compressive strength and modulus of PCL and PCL/TNT (0.5 wt%) composite scaffolds decreased during the 6 weeks of storage in SBF. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay and alkaline phosphates (ALP) activity results demonstrated that a generally increasing trend in cell viability was observed for PCL/TNT (0.5 wt%) scaffold sintered at 60 °C for 90 min compared to the control group. Eventually, the quantitative RT-PCR data provided the evidence that the PCL scaffold containing TiO2 nanotube constitutes a good substrate for cell differentiation leading to ECM mineralization

Height dependence of the penumbral fine-scale structure in the inner solar atmosphere

We studied the physical parameters of the penumbra in a large and fully-developed sunspot, one of the largest over the last two solar cycles, by using full-Stokes measurements taken at the photospheric Fe I 617.3 nm and chromospheric Ca II 854.2 nm lines with the Interferometric Bidimensional Spectrometer. Inverting measurements with the NICOLE code, we obtained the three-dimensional structure of the magnetic field in the penumbra from the bottom of the photosphere up to the middle chromosphere. We analyzed the azimuthal and vertical gradient of the magnetic field strength and inclination. Our results provide new insights on the properties of the penumbral magnetic fields in the chromosphere at atmospheric heights unexplored in previous studies. We found signatures of the small-scale spine and intra-spine structure of both the magnetic field strength and inclination at all investigated atmospheric heights. In particular, we report typical peak-to-peak variations of the field strength and inclination of $\approx 300$ G and $\approx 20^{\circ}$, respectively, in the photosphere, and of $\approx 200$ G and $\approx 10^{\circ}$ in the chromosphere. Besides, we estimated the vertical gradient of the magnetic field strength in the studied penumbra: we find a value of $\approx 0.3$ G km$^{-1}$ between the photosphere and the middle chromosphere. Interestingly, the photospheric magnetic field gradient changes sign from negative in the inner to positive in the outer penumbra.Comment: 14 page, 9 figures, accepted for Ap

A central line care maintenance bundle for the prevention of central line–associated bloodstream infection in non–intensive care unit settings

OBJECTIVE: To evaluate a central line care maintenance bundle to reduce central line-associated bloodstream infection (CLABSI) in non-ICU settings. DESIGN: Before-after trial with 12 month follow-up period. SETTING: 1250-bed teaching hospital. PARTICIPANTS: Patients with central lines on eight general medicine wards. Four wards received the intervention and four served as controls. INTERVENTION: A multifaceted catheter care maintenance bundle consisting of educational programs for nurses, update of hospital policies, visual aids, a competency assessment, process monitoring, regular progress reports, and consolidation of supplies necessary for catheter maintenance. RESULTS: Data were collected for 25,542 catheter-days including 43 CLABSI (rate = 1.68 per 1,000 CL-days) and 4,012 catheter dressing observations. Following the intervention, a 2.5% monthly decrease in the CLABSI incidence density was observed on intervention floors, but this was not statistically significant (95% confidence interval (CI); −5.3 – 0.4). On control floors, there was a smaller, but marginally significant decrease in CLABSI incidence during the study (change in monthly rate = −1.1%; 95% CI, −2.1 - −0.1). Implementation of the bundle was associated with improvement in catheter dressing compliance on intervention wards (78.8% compliance pre-intervention vs. 87.9% during intervention/follow-up; p<0.001) but improvement was also observed on control wards (84.9% compliance pre-intervention vs. 90.9% during intervention/follow-up; P = .001). CONCLUSIONS: A multi-faceted program to improve catheter care was associated with improvement in catheter dressing care, but no change in CLABSI rates. Additional study is needed to determine strategies to prevent CLABSI in non-ICU patients