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Structure of the novel membrane-coating material in proton-secreting epithelial cells and identification as an H+ATPase.
Specialized proton-secreting cells known collectively as mitochondria-rich cells are found in a variety of transporting epithelia, including the kidney collecting duct (intercalated cells) and toad and turtle urinary bladders. These cells contain a population of characteristic tubulovesicles that are believed to be involved in the shuttling of proton pumps (H+ATPase) to and from the plasma membrane. These transporting vesicles have a dense, studlike material coating the cytoplasmic face of their limiting membranes and similar studs are also found beneath parts of the plasma membrane. We have recently shown that this membrane coat does not contain clathrin. The present study was performed to determine the structure of this coat in rapidly frozen and freeze-dried tissue, and to determine whether the coat contains a major membrane protein transported by these vesicles, a proton pumping H+ATPase. The structure of the coat was examined in proton-secreting, mitochondria-rich cells from toad urinary bladder epithelium by rapidly freezing portions of apical membrane and associated cytoplasm that were sheared away from the remainder of the cell using polylysine-coated coverslips. Regions of the underside of these apical membranes as large as 0.2 micron2 were decorated by studlike projections that were arranged into regular hexagonal arrays. Individual studs had a diameter of 9.5 nm and appeared to be composed of multiple subunits arranged around a central depression, possibly representing a channel. The studs had a density of approximately 16,800 per micron2 of membrane. Similar arrays of studs were also found on vesicles trapped in the residual band of cytoplasm that remained attached to the underside of the plasma membrane, but none were seen in adjacent granular cells. To determine whether these arrays of studs contained H+ATPase molecules, we examined a preparation of affinity-purified bovine medullary H+ATPase, using the same technique, after incorporation of the protein eluted from a monoclonal antibody affinity column into phospholipid liposomes. The affinity-purified protein was shown to be capable of ATP-dependent acidification. In such preparations, large paracrystalline arrays of studs identical in appearance to those seen in situ were found. The dimensions of the studs as well as the number per square micrometer of membrane were identical to those of toad bladder mitochondria-rich cells: 9.5 nm in diameter, 16,770 per micron2 of membrane.(ABSTRACT TRUNCATED AT 400 WORDS
Uncertainties of the CJK 5 Flavour LO Parton Distributions in the Real Photon
Radiatively generated, LO quark (u,d,s,c,b) and gluon densities in the real,
unpolarized photon, calculated in the CJK model being an improved realization
of the CJKL approach, have been recently presented. The results were obtained
through a global fit to the experimental F2^gamma data. In this paper we
present, obtained for the very first time in the photon case, an estimate of
the uncertainties of the CJK parton distributions due to the experimental
errors. The analysis is based on the Hessian method which was recently applied
in the proton parton structure analysis. Sets of test parametrizations are
given for the CJK model. They allow for calculation of its best fit parton
distributions along with F2^gamma and for computation of uncertainties of any
physical value depending on the real photon parton densities. We test the
applicability of the approach by comparing uncertainties of example
cross-sections calculated in the Hessian and Lagrange methods. Moreover, we
present a detailed analysis of the chi^2 of the CJK fit and its relation to the
data. We show that large chi^2/DOF of the fit is due to only a few of the
experimental measurements. By excluding them chi^2/DOF approx 1 can be
obtained.Comment: 28 pages, 8 eps figures, 2 Latex figures; FORTRAN programs available
at http://www.fuw.edu.pl/~pjank/param.html; table 10, figure 10 and section 6
correcte
Testing Color Evaporation in Photon-Photon Production of J/Psi at CERN LEP II
The DELPHI Collaboration has recently reported the measurement of J/Psi
production in photon-photon collisions at LEP II. These newly available data
provide an additional proof of the importance of colored c bar{c} pairs for the
production of charmonium because these data can only be explained by
considering resolved photon processes. We show here that the inclusion of color
octet contributions to the J/Psi production in the framework of the color
evaporation model is able to reproduce this data. In particular, the
transverse-momentum distribution of the J/Psi mesons is well described by this
model.Comment: 10 pages, 5 Figures, Revtex
Interaction of small size wave packet with hadron target
We calculate in QCD the cross section for the scattering of an energetic
small-size wave packet off a hadron target. We use our results to study the
small- behaviour of , the distribution over cross
section for the pion, in the leading -order.Comment: Revised version of the report CEBAF-TH-96-0
Leading Chiral Contributions to the Spin Structure of the Proton
The leading chiral contributions to the quark and gluon components of the
proton spin are calculated using heavy-baryon chiral perturbation theory.
Similar calculations are done for the moments of the generalized parton
distributions relevant to the quark and gluon angular momentum densities. These
results provide useful insight about the role of pions in the spin structure of
the nucleon, and can serve as a guidance for extrapolating lattice QCD
calculations at large quark masses to the chiral limit.Comment: 8 pages, 2 figures; a typo in Ref. 7 correcte
Asymptotics and local constancy of characters of p-adic groups
In this paper we study quantitative aspects of trace characters
of reductive -adic groups when the representation varies. Our approach
is based on the local constancy of characters and we survey some other related
results. We formulate a conjecture on the behavior of relative to
the formal degree of , which we are able to prove in the case where
is a tame supercuspidal. The proof builds on J.-K.~Yu's construction and the
structure of Moy-Prasad subgroups.Comment: Proceedings of Simons symposium on the trace formul
Structure of the novel membrane-coating material in proton-secreting epithelial cells and identification as an H+ATPase.
Specialized proton-secreting cells known collectively as mitochondria-rich cells are found in a variety of transporting epithelia, including the kidney collecting duct (intercalated cells) and toad and turtle urinary bladders. These cells contain a population of characteristic tubulovesicles that are believed to be involved in the shuttling of proton pumps (H+ATPase) to and from the plasma membrane. These transporting vesicles have a dense, studlike material coating the cytoplasmic face of their limiting membranes and similar studs are also found beneath parts of the plasma membrane. We have recently shown that this membrane coat does not contain clathrin. The present study was performed to determine the structure of this coat in rapidly frozen and freeze-dried tissue, and to determine whether the coat contains a major membrane protein transported by these vesicles, a proton pumping H+ATPase. The structure of the coat was examined in proton-secreting, mitochondria-rich cells from toad urinary bladder epithelium by rapidly freezing portions of apical membrane and associated cytoplasm that were sheared away from the remainder of the cell using polylysine-coated coverslips. Regions of the underside of these apical membranes as large as 0.2 micron2 were decorated by studlike projections that were arranged into regular hexagonal arrays. Individual studs had a diameter of 9.5 nm and appeared to be composed of multiple subunits arranged around a central depression, possibly representing a channel. The studs had a density of approximately 16,800 per micron2 of membrane. Similar arrays of studs were also found on vesicles trapped in the residual band of cytoplasm that remained attached to the underside of the plasma membrane, but none were seen in adjacent granular cells. To determine whether these arrays of studs contained H+ATPase molecules, we examined a preparation of affinity-purified bovine medullary H+ATPase, using the same technique, after incorporation of the protein eluted from a monoclonal antibody affinity column into phospholipid liposomes. The affinity-purified protein was shown to be capable of ATP-dependent acidification. In such preparations, large paracrystalline arrays of studs identical in appearance to those seen in situ were found. The dimensions of the studs as well as the number per square micrometer of membrane were identical to those of toad bladder mitochondria-rich cells: 9.5 nm in diameter, 16,770 per micron2 of membrane.(ABSTRACT TRUNCATED AT 400 WORDS
Nuclear Effects in Charmonium Production in QCD
It is shown that the nuclear shadowing of charmonium due to the modification
of the nuclear parton distribution is similar in the factorization approach
based on non relativistic QCD and in the color evaporation model. In the first
model, a separate study of the color octet and color singlet contributions to
the yields of the various charmonium states as well as the contributions of
these states to the total production is performed. It is found a clear
dependence of these contributions which can reproduce experimental data
for moderate .Comment: 11 pages, 5 Postscript figure
Next-to-leading order QCD corrections to one hadron-production in polarized pp collisions at RHIC
We calculate the next-to-leading order QCD corrections to the spin-dependent
cross section for single-inclusive hadron production in hadronic collisions.
This process will be soon studied experimentally at RHIC, providing a tool to
unveil the polarized gluon distribution . We observe a considerably
improvement in the perturbative stability for both unpolarized and polarized
cross sections. The NLO corrections are found to be non-trivial, resulting in a
reduction of the asymmetry.Comment: 8 pages, RevTeX4, 9 figures include
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