Penggunaan Berbagai Macam Legum Cover Crop (LCC) Dalam Menyerap Logam-Logam Berat Pada Lahan Bekas Tambang Emas Di Kabupaten Sijunjung

Penelitian penggunaan berbagai macam Legum Cover Crop (LCC) dalammenyerap logam-logam berat pada lahan bekas tambang emas di kabupatensijunjung. Tujuan penelitian adalah untuk melihat LCC yang paling bagus dalammenyerap logam-logam berat pada lahan bekas tambang emas. Penelitian inidilakukan di Nagari Pala Luar Kecamatan Koto Tujuh Kabupaten Sijunjungselama 3 bulan dan dilanjutkan dengan analisis di Laboratorium Tanah FakultasPertanian Universitas Andalas. Rancangan yang digunakan dalam penelitian iniadalah Rancangan Acak Kelompok (RAK) 5x3 dengan 5 perlakuan dan 3ulangan, perlakuan penggunaan tanaman LCC dimana, A=Kontrol (tanpa LCC), B = Mucuna conchinchinensis, C = Calopogonium mucunoide, D = Centrocemapubescen, E = Mucuna bracteata. Data analisis dengan menggunakan tabelAnova 5% apabila F hitung lebih hitung lebih dari F tabel 5 % dan uji lanjut BNJ5%. Dari hasil penelitian tersebut dapat disimpulkan bahwa penggunaan LCCjenis Mucuna bracteata mampu menyerap logam berat lebih banyak dibandingkan jenis LCC lainnya.Kata Kunci : LCC, Logam-logam berat, lahan bekas tamban

Fitoremediasi Lahan Bekas Tambang Emas Dengan Penggunaan Berbagai Jenis Legume Dalam Memperbaiki Sifat Fisik Tanah Di Kabupaten Sijunjung

Kabupaten Sijunjung, Sumatera Barat, memiliki cadangan emas yang diperkirakan ada di beberapa lokasi. Kegiatan penambangan umumnya dilakukan di lahan pertanian dan perkebunan seperti persawahan dan perkebunan karet dengan teknik penambangan terbuka yang berpotensi merusak lingkungan. Akibat pengerukan, penimbunan, dan pemadatan kendaraan berat merusak tekstur tanah, pada tata air, resapan air yang terganggu akan meningkatkan limpasan dan erosi. Strategi perbaikan sifat fisik tanah dilakukan dengan penanaman tanaman penutup tanah yang dikenal dengan Legum Cover Crop (LCC). Tanaman ini dapat hidup dan bertahan hidup di tanah yang rusak dan berguna untuk melindungi tanah dari kerusakan erosi dan menghasilkan bahan organik dalam jumlah besar. Penelitian ini bertujuan untuk memperbaiki sifat fisik lahan bekas penambangan emas. Penelitian ini dilakukan di Nagari Pala Luar Kecamatan Koto Tujuh Kabupaten Sijunjung Sumatera Barat selama 3 bulan dan dilakukan analisis tanah di Laboratorium Tanah Fakultas Pertanian Universitas Andalas. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan 5 perlakuan LCC yang berbeda: A = Kontrol (tanpa penerapan LCC), B = Mucuna conchinchinensis, C = Calopogonium mucunoide, D = Centrocema pubescens dan E = Mucuna bracteata. Data yang terkumpul dianalisis menggunakan Analisis Varians (ANOVA) dan uji Tukey's HSD diterapkan jika ada perbedaan rata-rata. Hasil penelitian menunjukkan bahwa penggunaan LCC Mucuna conchinchinensis telah memperbaiki sifat fisik tanah yang ditunjukkan dengan peningkatan bahan organik tanah, penurunan volume tanah (BV), dan peningkatan total pori-pori tanah bekas tambang emas di Sijunjung. Kabupaten, Sumatera Barat

The two-step treatment for giant hepatic hemangiomas

The aim of the present study is to analyze the feasibility and the impact of a two-step approach in the treatment of giant hemangiomas (GH) i.e., exceeding 10 cm in maximum diameter, con-sisting of transarterial embolization (TAE) followed by laparoscopic liver resection (LLR). Ten patients with 11 GH were treated with TAE and subsequent LLR between 2017 and 2020 (Group A). A matched cohort of 10 patients with GH treated with upfront LLR between 2014 and 2017 was identified for comparison (Group B). Data were analyzed regarding intraoperative and postoperative outcomes, including successful completion of LLR, morbidity, and mortality. Successful microparticle emboliza-tion of the GH-feeding arteries was performed in all patients in group A. In three cases a liquid embolic agent (Squid-18) was also injected to obtain complete embolization. No complications were observed after TAE. Successful surgery was performed after a mean time interval of 2.2 days from TAE without any case of conversion to laparotomy. Statistically significant differences between group A and group B were found in intraoperative blood loss (250 \ub1 200 vs. 400 \ub1 300 mL, p = 0.039), operative time (245 \ub1 60 vs. 420 \ub1 60 min, p = 0.027), and length of stay (5 \ub1 1 vs. 8 \ub1 2 days, p = 0.046). Our data suggest that two-step TAE + LLR might be a safe and effective option for surgical treatment of GH >10 cm

Characterization of transcriptome dynamics during watermelon fruit development: sequencing, assembly, annotation and gene expression profiles

<p>Abstract</p> <p>Background</p> <p>Cultivated watermelon [<it>Citrullus lanatus </it>(Thunb.) Matsum. & Nakai var. <it>lanatus</it>] is an important agriculture crop world-wide. The fruit of watermelon undergoes distinct stages of development with dramatic changes in its size, color, sweetness, texture and aroma. In order to better understand the genetic and molecular basis of these changes and significantly expand the watermelon transcript catalog, we have selected four critical stages of watermelon fruit development and used Roche/454 next-generation sequencing technology to generate a large expressed sequence tag (EST) dataset and a comprehensive transcriptome profile for watermelon fruit flesh tissues.</p> <p>Results</p> <p>We performed half Roche/454 GS-FLX run for each of the four watermelon fruit developmental stages (immature white, white-pink flesh, red flesh and over-ripe) and obtained 577,023 high quality ESTs with an average length of 302.8 bp. <it>De novo </it>assembly of these ESTs together with 11,786 watermelon ESTs collected from GenBank produced 75,068 unigenes with a total length of approximately 31.8 Mb. Overall 54.9% of the unigenes showed significant similarities to known sequences in GenBank non-redundant (nr) protein database and around two-thirds of them matched proteins of cucumber, the most closely-related species with a sequenced genome. The unigenes were further assigned with gene ontology (GO) terms and mapped to biochemical pathways. More than 5,000 SSRs were identified from the EST collection. Furthermore we carried out digital gene expression analysis of these ESTs and identified 3,023 genes that were differentially expressed during watermelon fruit development and ripening, which provided novel insights into watermelon fruit biology and a comprehensive resource of candidate genes for future functional analysis. We then generated profiles of several interesting metabolites that are important to fruit quality including pigmentation and sweetness. Integrative analysis of metabolite and digital gene expression profiles helped elucidating molecular mechanisms governing these important quality-related traits during watermelon fruit development.</p> <p>Conclusion</p> <p>We have generated a large collection of watermelon ESTs, which represents a significant expansion of the current transcript catalog of watermelon and a valuable resource for future studies on the genomics of watermelon and other closely-related species. Digital expression analysis of this EST collection allowed us to identify a large set of genes that were differentially expressed during watermelon fruit development and ripening, which provide a rich source of candidates for future functional analysis and represent a valuable increase in our knowledge base of watermelon fruit biology.</p