Vascular Health in American Football Players: Cardiovascular Risk Increased in Division III Players

Studies report that football players have high blood pressure (BP) and increased cardiovascular risk. There are over 70,000 NCAA football players and 450 Division III schools sponsor football programs, yet limited research exists on vascular health of athletes. This study aimed to compare vascular and cardiovascular health measures between football players and nonathlete controls. Twenty-three athletes and 19 nonathletes participated. Vascular health measures included flow-mediated dilation (FMD) and carotid artery intima-media thickness (IMT). Cardiovascular measures included clinic and 24 hr BP levels, body composition, VO2 max, and fasting glucose/cholesterol levels. Compared to controls, football players had a worse vascular and cardiovascular profile. Football players had thicker carotid artery IMT (0.49 ± 0.06 mm versus 0.46 ± 0.07 mm) and larger brachial artery diameter during FMD (4.3 ± 0.5 mm versus 3.7 ± 0.6 mm), but no difference in percent FMD. Systolic BP was significantly higher in football players at all measurements: resting (128.2 ± 6.4 mmHg versus 122.4 ± 6.8 mmHg), submaximal exercise (150.4 ± 18.8 mmHg versus 137.3 ± 9.5 mmHg), maximal exercise (211.3 ± 25.9 mmHg versus 191.4 ± 19.2 mmHg), and 24-hour BP (124.9 ± 6.3 mmHg versus 109.8 ± 3.7 mmHg). Football players also had higher fasting glucose (91.6 ± 6.5 mg/dL versus 86.6 ± 5.8 mg/dL), lower HDL (36.5±11.2 mg/dL versus 47.1±14.8 mg/dL), and higher body fat percentage (29.2±7.9% versus 23.2±7.0%). Division III collegiate football players remain an understudied population and may be at increased cardiovascular risk

Effects of Dietary and Plasma Lipid Levels on Vascular Health Measures

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Synthesis of Oleoylethanolamide Using Lipase

An effective process for the enzymatic synthesis of oleoylethanolamide is described in this study. The process included purification of a commercial oleic acid product and then optimization of the reaction between the purified oleic acid and ethanolamine in the presence of hexane and a lipase. Under the optimal amidation reaction conditions identified, oleoylethanolamide was obtained with 96.6% purity. The synthesis was also conducted on a large scale (50 mmol of each of the reactants), and oleoylethanolamide purity and yield after crystallization purification were 96.1 and 73.5%, respectively. Compared to the previous studies, the current method of preparing high-purity oleoylethanolamide is more effective and economically feasible. The scalability and ease for such synthesis make it possible to study the biological and nutritional functions of the cannabinoid-like oleoylethanolamide in animal or human subjects

Visit-to-visit and 24-h blood pressure variability: association with endothelial and smooth muscle function in African Americans

The purpose of this study was to investigate the association of visit-to-visit and 24-h blood pressure (BP) variability with markers of endothelial injury and vascular function. We recruited 72 African Americans who were non-diabetic, non-smoking and free of cardiovascular (CV) and renal disease. Office BP was measured at three visits and 24-h ambulatory BP monitoring was conducted to measure visit-to-visit and 24-h BP variability, respectively. The 5-min time-course of brachial artery flow-mediated dilation and nitroglycerin-mediated dilation were assessed as measures of endothelial and smooth muscle function. Fasted blood samples were analyzed for circulating endothelial microparticles (EMPs). Significantly lower CD31+CD42− EMPs were found in participants with high visit-to-visit systolic blood pressure (SBP) variability or high 24-h diastolic blood pressure (DBP) variability. Participants with high visit-to-visit DBP variability had significantly lower flow-mediated dilation and higher nitroglycerin-mediated dilation at multiple time-points. When analyzed as continuous variables, 24-h mean arterial pressure variability was inversely associated with CD62+ EMPs; visit-to-visit DBP variability was inversely associated with flow-mediated dilation normalized by smooth muscle function and was positively associated with nitroglycerin-mediated dilation; and 24-h DBP variability was positively associated with nitroglycerin-mediated dilation. All associations were independent of age, gender, body mass index and mean BP. In conclusion, in this cohort of African Americans visit-to-visit and 24-h BP variability were associated with measures of endothelial injury, endothelial function and smooth muscle function. These results suggest that BP variability may influence the pathogenesis of CV disease, in part, through influences on vascular health

Racial differences in tumor necrosis factor-α-induced endothelial microparticles and interleukin-6 production

Michael D Brown1,3, Deborah L Feairheller1, Sunny Thakkar1, Praveen Veerabhadrappa1, Joon-Young Park21Hypertension, Molecular and Applied Physiology Laboratory, 2Cardiovascular Genomics Laboratory, Department of Kinesiology, 3Cardiovascular Research Center, School of Medicine, Temple University, Philadelphia, PA, USAAbstract: African Americans (AA) tend to have heightened systemic inflammation and endothelial dysfunction. Endothelial microparticles (EMP) are released from activated/apoptotic endothelial cells (EC) when stimulated by inflammation. The purpose of our study was to assess EMP responses to inflammatory cytokine (TNF-α) and antioxidant (superoxide dismutase, SOD) conditions in human umbilical vein ECs (HUVECs) obtained from AA and Caucasians. EMPs were measured under four conditions: control (basal), TNF-α, SOD, and TNF-α + SOD. Culture supernatant was collected for EMP analysis by flow cytometry and IL-6 assay by ELISA. IL-6 protein expression was assessed by Western blot. AA HUVECs had greater EMP levels under the TNF- α condition compared to the Caucasian HUVECs (6.8 ± 1.1 vs 4.7% ± 0.4%, P = 0.04). The EMP level increased by 89% from basal levels in the AA HUVECs under the TNF-α condition (P = 0.01) compared to an 8% increase in the Caucasian HUVECs (P = 0.70). Compared to the EMP level under the TNF-α condition, the EMP level in the AA HUVECs was lower under the SOD only condition (2.9% ± 0.3%, P = 0.005) and under the TNF-α + SOD condition (2.1% ± 0.4%, P = 0.001). Basal IL-6 concentrations were 56.1 ± 8.8 pg/mL/µg in the AA and 30.9 ± 14.9 pg/mL/µg in the Caucasian HUVECs (P = 0.17), while basal IL-6 protein expression was significantly greater (P < 0.05) in the AA HUVECs. These preliminary observational results suggest that AA HUVECs may be more susceptible to the injurious effects of the proinflammatory cytokine, TNF-α.Keywords: endothelium, inflammation, endothelial microparticles, African American

Terpenoids VII. Absolute stereostructure of copaene: Tetrahedron Lett.

NRC publication: Ye

Copaene: Proc.Chem.Soc.

NRC publication: Ye

Effects of Cardiovascular Fitness and Body Composition on Maximal Core Temperature in Collegiate Football Players During Preseason

McClelland, JM, Godek, SF, Chlad, PS, Feairheller, DL, and Morrison, KE. Effects of cardiovascular fitness and body composition on maximal core temperature in collegiate football players during preseason. J Strength Cond Res 32(6): 1662–1670, 2018—This study evaluated the effects of body mass index (BMI) and aerobic fitness (V[Combining Dot Above]O2max) on maximal core temperature values (Tcmax) in 17 National Collegiate Athletic Association Division III football players during preseason. The subjects included 9 backs (BKs) and 8 linemen (LM). V[Combining Dot Above]O2max testing was performed 1 week before preseason. Core temperature was monitored by ingestible sensor every 10 minutes during practices on day 4 (D1), day 5 (D2), day 7 (D3), and postacclimatization on day 14 (D4). Wet bulb globe temperature (WBGT) was recorded on each collection day. Independent, paired t-tests and Pearson\u27s correlations were performed (α = 0.05). There were no significant correlations between V[Combining Dot Above]O2max and Tcmax on D1 (WBGT = 29.07° C) or D2 (WBGT = 30.93° C), but on D3 (WBGT = 31.39° C) there was a nonsignificant moderate negative correlation (r = −0.564, p = 0.090). There were no significant correlations between BMI and Tcmax on D1 or D2, but on D3 there was a nonsignificant moderate positive correlation (r = 0.596, p = 0.069). Paired t-tests revealed that overall Tcmax (D1–3) (38.56 ± 0.32° C) was statistically higher (p = 0.002) than D4 (38.16 ± 0.30° C). Independent t-tests between groups showed that the Tcmax values during preacclimatization (D1–D3) were significantly higher in LM (38.50 ± 0.37° C) than BKs (38.16 ± 0.35° C) (p = 0.007). V[Combining Dot Above]O2max was significantly lower (p = 0.006) in LM (36.89 ± 6.40 ml·kg−1·min−1) than BKs (47.44 ± 7.09 ml·kg−1·min−1), and BMI was significantly higher (p = 0.019) in LM (35.59 ± 4.00 kg·m−2) than BKs (28.68 ± 3.38 kg·m−2). The results of this study demonstrate that LM are significantly less fit than BKs and have a greater BMI. When WBGT was the highest on D3, the results suggest that those with lower V[Combining Dot Above]O2max and higher BMI experienced a higher Tcmax

Vascular Health and Fitness Levels in Metabolically Healthy and Unhealthy Obesity

Please see the pdf version of the abstract