Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription

Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to study both the possible relationship between these processes in cultured pre-B cells and the role played by heavy (H) chain (mu) protein in regulating gene rearrangement. Our analysis of pre-B cell cultures representing various stages of maturity revealed that transcription of each germline Ig locus precedes or is coincident with its rearrangement. Cell lines containing one functional rearranged H chain allele, however, continue to transcribe and to rearrange the allelic, unrearranged H chain locus. These cell lines appear to initiate but not terminate rearrangement events and therefore provide information about the requirements for activating rearrangement but not about allelic exclusion mechanisms

Depth from Monocular Images using a Semi-Parallel Deep Neural Network (SPDNN) Hybrid Architecture

Deep neural networks are applied to a wide range of problems in recent years. In this work, Convolutional Neural Network (CNN) is applied to the problem of determining the depth from a single camera image (monocular depth). Eight different networks are designed to perform depth estimation, each of them suitable for a feature level. Networks with different pooling sizes determine different feature levels. After designing a set of networks, these models may be combined into a single network topology using graph optimization techniques. This "Semi Parallel Deep Neural Network (SPDNN)" eliminates duplicated common network layers, and can be further optimized by retraining to achieve an improved model compared to the individual topologies. In this study, four SPDNN models are trained and have been evaluated at 2 stages on the KITTI dataset. The ground truth images in the first part of the experiment are provided by the benchmark, and for the second part, the ground truth images are the depth map results from applying a state-of-the-art stereo matching method. The results of this evaluation demonstrate that using post-processing techniques to refine the target of the network increases the accuracy of depth estimation on individual mono images. The second evaluation shows that using segmentation data alongside the original data as the input can improve the depth estimation results to a point where performance is comparable with stereo depth estimation. The computational time is also discussed in this study.Comment: 44 pages, 25 figure

Summary of electrical component development for a 400-hertz Brayton energy conversion system

Design, fabrication, and testing of 12-kilowatt inductor alternator, voltage regulator-exciter, and parasitic loading speed controller - summar

Modeling the RXTE light curve of η\eta Carinae from a 3-D SPH simulation of its binary wind collision

The very massive star system $\eta$ Carinae exhibits regular 5.54-year (2024-day) period disruptive events in wavebands ranging from the radio to X-ray. There is a growing consensus that these events likely stem from periastron passage of an (as yet) unseen companion in a highly eccentric ($\epsilon \sim 0.9$) orbit. This paper presents three-dimensional (3-D) Smoothed Particle Hydrodynamics (SPH) simulations of the orbital variation of the binary wind-wind collision, and applies these to modeling the X-ray light curve observed by the Rossi X-ray Timing Explorer (RXTE). By providing a global 3-D model of the phase variation of the density of the interacting winds, the simulations allow computation of the associated variation in X-ray absorption, presumed here to originate from near the apex of the wind-wind interaction cone. We find that the observed RXTE light curve can be readily fit if the observer's line of sight is within this cone along the general direction of apastron. Specifically, the data are well fit by an assumed inclination $i = 45^{\circ}$ for the orbit's polar axis, which is thus consistent with orbital angular momentum being along the inferred polar axis of the Homunculus nebula. The fits also constrain the position angle $\phi$ that an orbital-plane projection makes with the apastron side of the semi-major axis, strongly excluding positions $\phi < 9^{\circ}$ along or to the retrograde side of the axis, with the best fit position given by $\phi = 27^{\circ}$. Overall the results demonstrate the utility of a fully 3-D dynamical model for constraining the geometric and physical properties of this complex colliding-wind binary system.Comment: 5 pages, 4 figures, accepted to MNRAS Letter

Motor-starting characteristics of two inductor alternators

Motor starting characteristics of two inductor alternator

Changing Labor Market Opportunities for Women and the Quality of Teachers 1957-1992

School officials and policy makers have grown increasingly concerned about their ability to attract and retain talented teachers. A number of authors have shown that in recent years the brightest students at least those with the highest verbal and math scores on standardized tests are less likely to enter teaching. In addition, it is frequently claimed that the ability of schools to attract these top students has been steadily declining for years. There is, however, surprisingly little evidence measuring the extent to which this popular proposition is true. We have good reason to suspect that the quality of those entering teaching has fallen over time. Teaching has remained a predominately female profession for years; at the same time, the employment opportunities for talented women outside of teaching have soared. In this paper, we combine data from four longitudinal surveys of high school graduates spanning the years 1957-1992 to examine how the propensity for talented women to enter teaching has changed over time. We find that while the quality of the average new female teacher has fallen only slightly over this period, the likelihood that a female from the top of her high school class will eventually enter teaching has fallen dramatically from 1964 to 1992 by our estimation, from almost 20% to under 4%.