Mutagenic and antimutagenic properties of some lichen species grown in the Eastern Anatolia Region of Turkey

All the methanol extracts did not show mutagenic activity in Ames/Salmonella and Z. mays MI test systems. Furthermore, some extracts showed significant antimutagenic activity against 9-AA in Ames test system. Inhibition rates for 9-AA mutagenicity ranged from 25.51 % (P. furfuracea – 0.05 μg/plate) to 66.14 % (C. islandica – 0.05 μg/plate). In addition, all of the extracts showed significant antimutagenic activity against sodium azide (NaN₃) mutagenicity on MI values of Z. mays.Целью работы было изучить мутагенный и антимутагенный потенциал метанольных экстрактов Cetraria islandica (L.) Ach. (Parmeliaceae), Pseudevernia furfuracea (L.) Zopf (Parmeliaceae) и Xanthoparmelia somloënsis (Gyeln.) Hale (Parmeliaceae) – лишайников из восточной части Турции. Ни один из экстрактов не показал мутагенной активности в тестах Эймса и Z. mays MI. Более того, некоторые экстракты проявляли заметную антимутагенную активность против 9-амино-акридина в тесте Эймса. Уровень ингибирования варьировал от 25,51 % (P. furfuracea) до 66,14 % (C. islandica). Кроме того, все экстракты проявляли значительную антимутагенную активность против азида натрия в Z. mays MI тесте. Все экстракты могут считаться генотоксично безопасными в исследованных концентрациях

Application of random amplified polymorphic DNA (RAPD) to detect genotoxic effect of trifluralin on maize (Zea mays)

PubMed ID: 22413768Trifluralin is a widely used dinitroaniline herbicide throughout the world. However, limited efforts have been made to study its genotoxic effects on different plants. The present study aimed to evaluate the herbicide's genotoxic potential on maize (Zea mays) by using the random amplified polymorphic DNA (RAPD) technique. For this purpose, maize seedlings were treated with aqueous solutions of trifluralin at concentrations ranging from 0.5 to 3 ppm for 7 days. In the RAPD analyses, 15 primers were used and 91 bands were obtained, with an average of 6.06 bands per primer in the control seedlings. After trifluralin treatment, significant changes were observed in RAPD profiles. These changes included loss of normal bands and appearance of new bands, in comparison to the control group, and they were dose dependent. In addition, root growth and total soluble protein level in trifluralin-treated seedlings were analyzed and compared for genomic template stability (GTS), which was performed for the qualitative measurement of changes in randomly amplified polymorphic DNA (RAPD) profiles. The results showed that GTS, root growth, and total soluble protein content of the seedlings gradually decreased with an increase in trifluralin concentration. These findings suggest that the RAPD technique is a useful biomarker assay to evaluate the genotoxic effects of herbicides on plants. © 2013 Informa Healthcare USA, Inc

HLA-B27 allele frequency in a Turkish study population with primer osteoarthritis

38th Congress of the Federation-of-European-Biochemical-Societies (FEBS) -- JUL 06-11, 2013 -- Saint Petersburg, RUSSIAWOS: 000325919201415…Federat European Biochemical So

Analysis of the Codon 72 Polymorphism of P53 Gene in Patients With Multinodular Goiter to Develop Thyroid Cancer

22nd Biennial Congress of the European-Association-for-Cancer-Research -- JUL 07-10, 2012 -- Barcelona, SPAINWOS: 000313036501040…European Assoc Canc Re

Analysis of the codon 72 polymorphism of p53 gene in patients with multinodular goitre: A risk factor of thyroid cancer

Aim: In this study, it was aimed to determine whether the p53 gene codon 72 polymorphism is a genetic marker of thyroid cancer development in multinodular goitre patients. Methods: Genomic DNA was isolated from 90 persons (42 with multinodular goitre and 48 healthy controls) in the study. DNA was amplified with specific primers by PCR and RFLP technique was used to analyze p53 gene codon 72 polymorphism alleles and genotypes. PCR-RFLP products were assessed with UV transilluminator after application of agarose gel electrophoresis. Results: According to genotype distribution and allele frequencies of p53 gene codon 72 polymorphism, there was no significant difference between groups. Conclusions: As a result of our study we may assert that p53 gene codon 72 polymorphism should not be considered as a genetic marker to develop thyroid cancer in the studied Turkish population with multinodular goitre

Determination of chemical composition and genotoxic effects of essential oil obtained from Nepeta nuda on Zea mays seedlings

PubMed ID: 22312034We aimed to determine the genotoxic potential of essential oil (EO) obtained from Nepeta nuda. The chemical content of EO was measured via gas chromatography/mass spectrometry. The most abundant contents were 4a?,7?,7a?-nepetalactone (18.10%), germacrene (15.68%) and elemol (14.38%). For genotoxic effects of EO, Zea mays’ seeds were exposed to four different concentrations of this oil. Inhibition of root and stem growth were observed with an increase in EO concentrations. Randomly amplified polymorphic DNA (RAPD) method was used to determine the genotoxic effects of EO. Some changes occurred in RAPD profiles of germinated EO-treated seeds. Even though total soluble protein quantity vary, the data observed from the protein profiles of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) showed that there was a little differentiation between band profiles of treated samples and control group. We concluded that the basis of interactions between plants, like allelopathy, may be related with genotoxic effects of EO. © 2012, SAGE Publications. All rights reserved

Assessment of genotoxic effects of 2,4-dichlorophenoxyacetic acid on maize by using RAPD analysis

The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is principally used in agriculture and may pose toxic risks to some crops. Thus, the goal of the present study was to evaluate the genotoxic properties of 2,4-D by using randomly amplified polymorphic DNA (RAPD) assay in the root of maize (Zea mays L.) seedlings. For this purpose maize seedlings were treated with aqueous solutions of 2,4-D at concentrations ranging from 0.5. ppm to 2. ppm for 7 days. After the growth, root length, total soluble protein levels and RAPD profiles of the seedlings were analyzed to determine genotoxicity. The results showed that increasing concentrations of 2,4-D caused a decrease in the root length and an increase in the total soluble protein levels of the seedlings. The changes occurred in the RAPD profiles of the root tips following 2,4-D treatment included loss of normal bands and appearance of new bands in comparison to that of the control seedlings. Genomic template stability (GTS), carried out for the qualitative assessment of alterations in RAPD profiles, was also significantly affected by increasing concentrations of 2,4-D. © 2012 Elsevier B.V.

The protective role of zinc and calcium in Vicia faba seedlings subjected to cadmium stress

PubMed ID: 20921057The aim of the present study was to evidence the possible antagonistic effect of Zinc (Zn2+) and Calcium (Ca2+) against cadmium (Cd2+)-induced DNA damage by using random amplification of polymorphic DNA (RAPD) and metabolic activities in Vicia faba. The results showed that all doses of Cd2+(10-3M, 10-5M) caused an increase in polymorphism value and a decrease in genomic template stability (GTS %). In addition, when 10-4-10-6M Ca2+, 10-6M Zn2+were added together with 10-3M, 10-4M, 10-5M of Cd2+, polymorphism value decreased besides GTS, total protein and chlorophyll content increased. Results suggested that Zn2+and Ca2+have an antagonistic effect against Cd2+. The order of the antagonisms of Ca2+, Zn2+against Cd2+toxicity was Ca2+> Zn2+. Especially, the degree of antagonistic effect of Zn2+against Cd2+is probably related to its concentration ratio. © 2011, SAGE Publications. All rights reserved

The association Between Development and Progression of Multinodular Goiter and Thyroid-Stimulating hormone Receptor Gene D727E and P52T Polymorphisms

PubMed ID: 23336574Aim: This study has been performed on a Turkish population with multinodular goiter (MNG) to investigate the thyroid-stimulating hormone receptor (TSHR) gene D727E and P52T polymorphisms. Methods: DNA samples were isolated from 300 patients with MNG and 142 controls. Polymerase chain reaction-restriction fragment length polymorphism and agarose gel electrophoresis were used. Results: The D727E polymorphism G-allele frequency and the CG and GG genotypes were significantly higher in patients with MNG. However, there was no significant difference in the P52T polymorphism between patients and control subjects. Conclusions: As a conclusion, the D727E polymorphism G allele may be related to MNG development in the studied population. © 2013, Mary Ann Liebert, Inc

Protective effects of methanol extracts from Cladonia rangiformis and Umbilicaria vellea against known mutagens sodium azide and 9-aminoacridine

PubMed ID: 21427134Lichens and their various extracts have been occasionally used in the treatment of many diseases. Cladonia rangiformis and Umbilicaria vellea are two important species of these lichens and they have several biological activities. In the present study, methanol extracts of these lichens, which are grown in the Eastern Anatolia Region of Turkey, were isolated, and their mutagenic and antimutagenic properties were investigated by using AMES-Salmonella and Zea mays Root Tip Mitotic Index mutagenicity and antimutagenicity assay systems. Known mutagens sodium azide (NaN3) and 9-Aminoacridine (9-AA) were used to determine antimutagenic properties of methanol extracts. The results showed that all methanol extracts, investigated in the present study, can be considered genotoxically safe because they do not have mutagenic activity at the tested concentrations. Besides, all of them have antimutagenic activity against 9-AA known as a model intercalator agent in the AMES-Salmonella test system. The inhibition rates obtained from the antimutagenicity assays ranged from 37.07% (C. rangiformis—5 µg/plate) to 54.39% (C. rangiformis—5 µg/plate). Furthermore, all the methanol extracts have significant antimutagenic activity against NaN3mutagenicity in Z. mays Root Tip Mitotic Index assay system. These activities are valuable towards an extension of the employ of these drugs as new phytotherapeutic or preservative ingredients. © 2011, SAGE Publications. All rights reserved