Preparation of interactive educational and methodical providing: look from the student, from the teacher and from the developer

There is analysed some experience of chair of Applied mathematics of the Ural state economic university in the area of development and using the interactive educational and methodical providing.В работе проанализирован опыт кафедры Прикладной математики Уральского государственного экономического университета в области разработки и использования интерактивного учебно-методического обеспечения

BIOSYNTHESIS OF LYCORINE BY IN VITRO CULTURES OF PANCRATIUM MARITIMUM L. (AMARYLLIDACEAE)

ABSTRACT Seeds of Pancratium maritimum collected from the natural populations along Bulgaria

Interplay of quark and meson degrees of freedom in a near-threshold resonance

We investigate the interplay of quark and meson degrees of freedom in a physical state representing a near-threshold resonance for the case of a single continuum channel. We demonstrate that such a near-threshold resonance may possess quite peculiar properties if both quark and meson dynamics generate weakly coupled near-threshold poles in the S-matrix. In particular, the scattering t-matrix may possess zeros in this case. We also discuss possible implications for production reactions as well as studies within lattice QCD.Comment: LaTeX2e, 11 pages, minor typo corrections, to appear in Eur.Phys.J.

Assessment of the dynamics of mortality from alcohol-related causes at the regional level

Aim of the study was to investigate rates of mortality from the alcohol-related reasons among the population of the Republic of Buryatia and their dynamics during 2013–2017 for assessment of efficiency of the preventive actions directed to decrease in prevalence of scales of alcoholism. Material and methods. Medical statistics presented in official publications of the Rosstat and the Republican Medical Information and Analytical Center of the Ministry of Health of the Republic of Buryatia were used as an empirical base. Mortality rates are calculated on the average annual population. Results and discussion. 2974 persons died from the alcohol-associated reasons in the Republic during 2013-2017 according to Buryatstat. The rate of mortality decreased by 11.98 % on 100 thousand population (from 60.30 to 53.85). That exceeds similar indicators in the Far Eastern Federal District (further the FEFD) by 1.76 times, Siberian Federal District (further the SFD) by 1.52 times and the Russian Federation by 1.45 times. According to this indicator the Republic of Buryatia takes the 2nd rank place after Transbaikal region among 11 subjects included into the FEFD since 03.11.2018. Conclusions. Mortality indicator from the alcohol-associated reasons is one of the most informative indicators for assessment of the territories social and economic development and wellbeing. The decrease in mortality from the alcohol-associated reasons and volumes of alcoholic products consumption per caput is noted in recent years in the Republic. At the same time, the problem of increase in rates of growth of the mortality value from the alcohol-associated reasons among female population of working-age, living in rural areas allerts. Territories of risk with the high level of population alcoholic mortality and relating to «the most drinking», geographically being most far off from the capital of the Republic are revealed

Search for the Radiative Capture d+d->^4He+\gamma Reaction from the dd\mu Muonic Molecule State

A search for the muon catalyzed fusion reaction dd --> ^4He +\gamma in the dd\mu muonic molecule was performed using the experimental \mu CF installation TRITON and NaI(Tl) detectors for \gamma-quanta. The high pressure target filled with deuterium at temperatures from 85 K to 800 K was exposed to the negative muon beam of the JINR phasotron to detect \gamma-quanta with energy 23.8 MeV. The first experimental estimation for the yield of the radiative deuteron capture from the dd\mu state J=1 was obtained at the level n_{\gamma}\leq 2\times 10^{-5} per one fusion.Comment: 9 pages, 3 Postscript figures, submitted to Phys. At. Nuc

NITRILIUM DERIVATES OF NIDO-CARBORANE IN SYNTHESIS OF TRANSITIONAL METAL COMPLEXES

This work was supported by Russian Science Foundation, project № 21-73-10199

Type II restriction endonuclease R.Eco29kI is a member of the GIY-YIG nuclease superfamily

<p>Abstract</p> <p>Background</p> <p>The majority of experimentally determined crystal structures of Type II restriction endonucleases (REases) exhibit a common PD-(D/E)XK fold. Crystal structures have been also determined for single representatives of two other folds: PLD (R.BfiI) and half-pipe (R.PabI), and bioinformatics analyses supported by mutagenesis suggested that some REases belong to the HNH fold. Our previous bioinformatic analysis suggested that REase R.Eco29kI shares sequence similarities with one more unrelated nuclease superfamily, GIY-YIG, however so far no experimental data were available to support this prediction. The determination of a crystal structure of the GIY-YIG domain of homing endonuclease I-TevI provided a template for modeling of R.Eco29kI and prompted us to validate the model experimentally.</p> <p>Results</p> <p>Using protein fold-recognition methods we generated a new alignment between R.Eco29kI and I-TevI, which suggested a reassignment of one of the putative catalytic residues. A theoretical model of R.Eco29kI was constructed to illustrate its predicted three-dimensional fold and organization of the active site, comprising amino acid residues Y49, Y76, R104, H108, E142, and N154. A series of mutants was constructed to generate amino acid substitutions of selected residues (Y49A, R104A, H108F, E142A and N154L) and the mutant proteins were examined for their ability to bind the DNA containing the Eco29kI site 5'-CCGCGG-3' and to catalyze the cleavage reaction. Experimental data reveal that residues Y49, R104, E142, H108, and N154 are important for the nuclease activity of R.Eco29kI, while H108 and N154 are also important for specific DNA binding by this enzyme.</p> <p>Conclusion</p> <p>Substitutions of residues Y49, R104, H108, E142 and N154 predicted by the model to be a part of the active site lead to mutant proteins with strong defects in the REase activity. These results are in very good agreement with the structural model presented in this work and with our prediction that R.Eco29kI belongs to the GIY-YIG superfamily of nucleases. Our study provides the first experimental evidence for a Type IIP REase that does not belong to the PD-(D/E)XK or HNH superfamilies of nucleases, and is instead a member of the unrelated GIY-YIG superfamily.</p