617 research outputs found
Isolation and mapping of a C3'H gene (CYP98A49) from globe artichoke, and its expression upon UV-C stress
Globe artichoke represents a natural source of phenolic compounds with dicaffeoylquinic acids along with their biosynthetic precursor chlorogenic acid (5-caffeoylquinic acid) as the predominant molecules. We report the isolation and characterization of a full-length cDNA and promoter of a globe artichoke p-coumaroyl ester 3¿-hydroxylase (CYP98A49), which is involved in both chlorogenic acid and lignin biosynthesis. Phylogenetic analyses demonstrated that this gene belongs to the CYP98 family. CYP98A49 was also heterologously expressed in yeast, in order to perform an enzymatic assay with p-coumaroylshikimate and p-coumaroylquinate as substrates. Real Time quantitative PCR analysis revealed that CYP98A49 expression is induced upon exposure to UV-C radiation. A single nucleotide polymorphism in the CYP98A49 gene sequence of two globe artichoke varieties used for genetic mapping allowed the localization of this gene to linkage group 10 within the previously developed map
Non-resonant microwave absorption studies of superconducting MgB_2
Non-resonant microwave absorption(NRMA) studies of superconducting MgB_2 at a
frequency of 9.43 GHz in the field range -50 Gauss to 5000 Gauss are reported.
The NRMA results indicate near absence of intergranular weak links. A linear
temperature dependence of the lower critical field H_c1 is observed indicating
a non s-wave superconductivity. However, the phase reversal of the NRMA signal
which could suggest d-wave symmetry is also not observed.Comment: 8 pages, 2 figure
Critical State Flux Penetration and Linear Microwave Vortex Response in YBa_2Cu_3O_{7-x} Films
The vortex contribution to the dc field (H) dependent microwave surface
impedance Z_s = R_s+iX_s of YBa_2Cu_3O_{7-x} thin films was measured using
suspended patterned resonators. Z_s(H) is shown to be a direct measure of the
flux density B(H) enabling a very precise test of models of flux penetration.
Three regimes of field-dependent behavior were observed: (1) Initial flux
penetration occurs on very low field scales H_i(4.2K) 100Oe, (2) At moderate
fields the flux penetration into the virgin state is in excellent agreement
with calculations based upon the field-induced Bean critical state for thin
film geometry, parametrized by a field scale H_s(4.2K) J_c*d 0.5T, (3) for very
high fields H >>H_s, the flux density is uniform and the measurements enable
direct determination of vortex parameters such as pinning force constants
\alpha_p and vortex viscosity \eta. However hysteresis loops are in
disagreement with the thin film Bean model, and instead are governed by the low
field scale H_i, rather than by H_s. Geometric barriers are insufficient to
account for the observed results.Comment: 20 pages, LaTeX type, Uses REVTeX style files, Submitted to Physical
Review B, 600 dpi PostScript file with high resolution figures available at
http://sagar.physics.neu.edu/preprints.htm
Observation of Individual Josephson Vortices in YBCO Bicrystal Grain-boundary Junctions
The response of YBCO bicrystal grain-boundary junctions to small dc magnetic
fields (0 - 10 Oe) has been probed with a low-power microwave (rf) signal of
4.4 GHz in a microwave-resonator setup. Peaks in the microwave loss at certain
dc magnetic fields are observed that result from individual Josephson vortices
penetrating into the grain-boundary junctions under study. The system is
modeled as a long Josephson junction described by the sine-Gordon equation with
the appropriate boundary conditions. Excellent quantitative agreement between
the experimental data and the model has been obtained. Hysteresis effect of dc
magnetic field is also studied and the results of measurement and calculation
are compared.Comment: 11 pages, 4 figure
Location of chlorogenic acid biosynthesis pathway and polyphenol oxidase genes in a new interspecific anchored linkage map of eggplant
© Gramazio et al.; licensee BioMed Central. 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated
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