Recovery and increase of the productivity of wells of Kashirskiy and Podolskiy reservoirs of the certain Perm region oil field

The paper presents the results of the analysis of the efficiency of improved oil recovery (IOR) methods designed to restore and increase the productivity of wells of Kashirskiy and Podolskiy reservoirs of the certain Perm region oil field. Indirect evidences prove that the carbonate reservoir of the formations exhibits fracture-pore-type reservoir properties, which affects the productivity of wells and results of IOR methods. Comparative analysis of the efficiency of IOR methods implemented on production wells poined out on higher values for proppant hydraulic fracturing (HF). The increment in well production oil rate after HF increases with an increase in the specific consumption of proppant. The calculations are performed according to the wave acoustic cross-dipole logging (cross-dipole sonic). According to the results of studies and calculations, the profile of horizontal stresses in the reservoir was constructed, the values of bottomhole pressure at which the closure of the fracture occurs in individual layers are substantiated. A retrospective design of the main HF was performed for a certain well. It is showed that the height of fracture development is limited by dense barriers above and below the perforation interval, while the part of the fracture formed is not packed with proppant. Proppant HF is accompanied by a significant increase in well water-cut after IOR methods implementation, the nature of which changes in subsequent periods indicates a high probability of involvment of formation drainage through a fractured interbeds with low natural oil saturation to the process. The analysis of the results of IOR methods, well logging data, taking into account the built retrospective design of the main HF, leads to the conclusion that it is necessary to optimize the technological parameters while designing the HF for production wells of the Kashirskiy and Podolskiy reservoirs of the certain Perm region oil fields That is controlled by increasing the specific consumption of proppant, reducing the polymer load and the share of the buffer stage of the main HF

Mechanisms of trematodiases pathogenicity: the presence of the secretory proteins from the liver fluke Opisthorchis felineus in the gallbladder tissues of the patients with chronic opisthorchiasis

Opisthorchiasis caused by the liver fluke Opisthorchis felineus infection remains a serious public health problem in the former USSR and Eastern European countries. O. felineus infests the bile ducts, the liver and gallbladder of many fish-eating mammalian species, including humans. Opisthorchiasis leads to a number of related diseases of the liver and pancreas: liver fibrosis, cholangitis, cholecystitis, liver cysts and pancreatitis. Excretory-secretory products of the parasite are considered to be key factors in host-parasite relationships and mediate pathogenic pleiotropic effects on the host organism.The aim of this study was to determine the helminthic proteins (thioredoxin peroxidase and glutathione-S-transferase) in the gallbladder tissues of the experimental animals and patients with opisthorchiasis disease. We demonstrated by immunohistochemistry assay using antibodies against recombinant O. felineus proteins that thioredoxin peroxidase and glutathione-S-transferase could be detected in the biliary duct epithelium of the experimental animals and in human gallbladder tissues. Moreover, these proteins could also be detected in human gallbladder infiltrated cells and underlying connective tissues. The results are important for understanding the molecular mechanisms of opisthorchiasis pathogenesis, as well as for improvement of the immunodiagnostics of the opisthorchiasis and opisthorchiasis-related diseases

Correlation of susceptibility to ortho-aminoazotolueneinduced hepatocarcinogenesis with Car and Ahr signaling pathway activation in mice

Ortho-aminoazotoluene (OAT) is a potent hepatocarcinogen for most strains of mice. It has previously been shown that OAT application activates the aryl hydrocarbon receptor (Ahr) and the constitutive androstane receptor (Car) in the mouse liver. Both of these receptors are directly involved in the process of hepatocarcinogenesis. In this study, we investigated the effect of chronic OAT administration on the mRNA expression levels of Ahr, Car and their target genes Cyp1a1 and Cyp2b10 in the liver of DD/He (DD) and CC57BR/Mv (BR) mouse strains contrasting in sensitivity to hepatocarcinogenesis. The inflammatory response of these strains was also studied. Male mice of both strains received OAT oil solution at the dose of 225 mg/kg body weight four times within two months. Control animals received the equivalent solvent amount. Mice were sacrificed on days 1 and 4 after the last OAT administration. Gene expression levels in the liver were determined by real-time PCR. The inflammatory response was evaluated by serum concentration of tumor necrosis factor alpha (TNF-alpha). In resistant BR mice, OAT induced a pronounced and prolonged increase in Cyp1a1 mRNA, showing primarily Ahr activation, while the DD strain displayed a more pronounced elevation of Cyp2b10 expression, indicative of Car activation. In addition, a strong inflammatory response to OAT was recorded in DD mice but not in BR. It is assumed that the prevalence of Ahr signaling pathway activation over Car signaling pathway activation is a factor of resistance to OAT-induced hepatocarcinogenesis

The morphofunctional and biochemical characteristics of opisthorchiasis-associated cholangiocarcinoma in a Syrian hamster model

The validity of experimental models of pathologies is one of the key challenges in translational medicine. Cholangiocarcinoma, or bile duct cancer, ranks second among oncological diseases of the liver. There is a strong association between bile duct cancer and parasitic infestation of the liver caused by trematodes in the family Opisthorchiidae. We have recently demonstrated that cholangiocarcinoma can develop in Syrian hamsters (Mesocricetus auratus) infected by Opisthorchis felineus and administered with dimethylnitrosamine. However, there is still no description of how this experimental model can possibly be used in translational research. The aim of this work was to study the morphological, functional and biochemical characteristics during cholangiocarcinoma development in Syrian hamsters infected by O. felineus and administered with dimethylnitrosamine. The experi­ment lasted 30 weeks with combined exposure to dimethylnitrosamine in drinking water at a dose of 12.5 ppm and a single injection of 50 metacercariae O. felineus. It was shown that the development of cholangiocarcinoma (18 weeks) increased the total number of basophils, eosinophils and monocytes, the relative number of granulocytes, the amount of total and direct bilirubin, and cholesterol and ALT levels, but reduced the relative number of lymphocytes. Based on pathological, morphometric and biochemical analyses, our model has characteristics similar to those in patients with opisthorchiasisassociated cholangiocarcinoma. Thus, this model can be used to test anticancer drugs, to study the mechanisms of cholangiocarcinogenesis and to search for molecular markers for early diagnosis of bile duct cancer

Коррекция микроциркуляторных нарушений в комплексном лечении больных с острой эмпиемой плевры и пиопневмотораксом

Results of treatment of 548 patients with acute empyema and pyopneumothorax are shown. An application of freshly frozen or cryosupernatant plasma, heparin or proteinase inhibitors provides an improvement in microcirculation at the inflammation area and allows better outcome equally with the adequate drainage of the pleural space.Дан анализ результатов лечения 548 больных с острой эмпиемой плевры и пиопневмотораксом. Применение свежезамороженной или криосупернатантной плазмы, введение гепарина и ингибиторов протеиназ способствует улучшению микроциркуляции в очаге воспаления, что наряду с адекватным дренированием гнойника в плевральной полости и рациональной антибиотикотерапией позволяет улучшить исходы заболевания