36 research outputs found
C4BQ0: a genetic marker of familial HCV-related liver cirrhosis.
Source
Department of Medicine and Pneumology, V Cervello Hospital, Via Trabucco 180, 90146 Palermo, Italy. [email protected]
Abstract
BACKGROUND AND METHODS:
Host may have a role in the evolution of chronic HCV liver disease. We performed two cross-sectional prospective studies to evaluate the prevalence of cirrhosis in first degree relatives of patients with cirrhosis and the role of two major histocompatibility complex class III alleles BF and C4 versus HCV as risk factors for familial clustering.
FINDINGS:
Ninety-three (18.6%) of 500 patients with cirrhosis had at least one cirrhotic first degree relative as compared to 13 (2.6%) of 500 controls, (OR 7.38; CI 4.21-12.9). C4BQ0 was significantly more frequent in the 93 cirrhotic patients than in 93 cirrhotic controls without familiarity (Hardy-Weinberg equilibrium: chi2 5.76, P = 0.016) and in 20 families with versus 20 without aggregation of HCV related cirrhosis (29.2% versus 11.3%, P = 0.001); the association C4BQ0-HCV was found almost only in cirrhotic patients with a family history of liver cirrhosis.
CONCLUSIONS:
Our studies support the value of C4BQ0 as a risk indicator of familial HCV related cirrhosis
LA LIPIDOMICA DELLA NON ALCOHOLIC FATTY LIVER DISEASE: ANALISI DELLA CINETICA DELL\u2019ACIDO PALMITICO MEDIANTE L\u2019USO DI UN ISOTOPO STABILE IN UN MODELLO IN VITRO
Salinomycin and other ionophores as a new class of antimalarial drugs with transmission-blocking activity
The drug target profile proposed by the Medicines for Malaria Venture for a malaria elimination/eradication policy focuses on molecules active on both asexual and sexual stages of Plasmodium, thus with both curative and transmission-blocking activities. The aim of the present work was to investigate whether the class of monovalent ionophores, which includes drugs used in veterinary medicine and that were recently proposed as human anticancer agents, meets these requirements. The activity of salinomycin, monensin, and nigericin on Plasmodium falciparum asexual and sexual erythrocytic stages and on the development of the Plasmodium berghei and P. falciparum mosquito stages is reported here. Gametocytogenesis of the P. falciparum strain 3D7 was induced in vitro, and gametocytes at stage II and III or stage IV and V of development were treated for different lengths of time with the ionophores and their viability measured with the parasite lactate dehydrogenase (pLDH) assay. The monovalent ionophores efficiently killed both asexual parasites and gametocytes with a nanomolar 50% inhibitory concentration (IC50). Salinomycin showed a fast speed of kill compared to that of standard drugs, and the potency was higher on stage IV and V than on stage II and III gametocytes. The ionophores inhibited ookinete development and subsequent oocyst formation in the mosquito midgut, confirming their transmission-blocking activity. Potential toxicity due to hemolysis was excluded, since only infected and not normal erythrocytes were damaged by ionophores. Our data strongly support the downstream exploration of monovalent ionophores for repositioning as new antimalarial and transmission-blocking leads
The T.O.S.C.A. Project: Research, Education and Care
Despite recent and exponential improvements in diagnostic-
therapeutic pathways, an existing βGAPβ has been revealed
between the βreal world careβ and the βoptimal careβ
of patients with chronic heart failure (CHF). We present the
T.O.S.CA. Project (Trattamento Ormonale dello Scompenso
CArdiaco), an Italian multicenter initiative involving different
health care professionals and services aiming to explore the
CHF βmetabolic pathophysiological modelβ and to improve
the quality of care of HF patients through research and continuing
medical education
Role of prothrombotic polymorphisms in successful or unsuccessful aging
The study of the genetic profile of
centenarians aims to identify the genes and allelic variants which may influence a greater life expectancy
and that can be considered as predisposing factors associated to the aging diseases, such as Alzheimer. Centenarians, that represent a cohort of
selected survivors, show an hypercoagulability state characterised by striking signs of high coagulation
enzyme activity, as directly assessed by the tested higher plasma level of some important factors involved in the haemostasis balance. Anyway, these
individuals seem to have a reduced susceptibility to dementia, as well as to cardiovascular events. In this
study we analyze the frequencies of Leiden Factor V polymorphism (G1691A), and G20210A of prothrombin (FII) in three cohorts of subjects: patients
with Alzheimer\u2019s disease (unsuccessful aging), nonagenarians (successful aging) and young healthy controls, to assess whether allelic variants associated to the modification of haemostatic system function,
may play a role in the protection or susceptibility to Alzheimer disease, as well as to reach a successful aging. No significant differences were observed in the frequencies of the three groups studied. These results indicate that the presence or absence of the gene
variants examined did not influence the achievement of advanced age and are not risk factors for Alzheimer\u2019s disease. The state of hypercoagulability and the
possession of these risk alleles appear to be compatible with the achievement of longevity and are not implied as risk factors in Alzheimer disease
development
ΠΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ Π΄Π΅ΠΉΡΡΠ²ΠΈΠΉ ΠΏΡΠΈ Π²ΠΈΠ·ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΊΡΠΎΠ²ΠΎΠΈΠ·Π»ΠΈΡΠ½ΠΈΠΉ/Π³Π΅ΠΌΠ°ΡΠΎΠΌ ΠΌΡΠ³ΠΊΠΈΡ ΡΠΊΠ°Π½Π΅ΠΉ Ρ ΠΏΠΎΠΆΠΈΠ»ΡΡ Π±ΠΎΠ»ΡΠ½ΡΡ , ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ SARS-CoV-2
Aim. To determine ultrasound, computed tomography and angiographic image characteristics for soft tissue hemorrhages/hematomas, the sequence of using imaging methods in patients infected with SARS-CoV-2, to study the morphology of changes in soft tissues, to determine the essence of the concept and to develop treatment tactics for this complication of COVID-19.Material and methods. During 4 months of treatment of elderly patients (+60) infected with SARS-CoV-2, 40 patients were identified with soft tissue hemorrhages/hematomas, of which 26 (65%) patients with large hematomas (>10 cm in size and > 1000 ml in volume). The analysis of clinical and laboratory parameters, methods of instrumental diagnostics (ultrasound β 26 patients, CT β 10 patients, angiography β 9 patients, punctures β 6 patients) was carried out; autopsy material was studied in 11 cases.Results. Image characteristics of hemorrhages/hematomas of soft tissue density were obtained using modern instrumental methods, and the sequence of application of visualization methods was determined. A tactic for managing a patient with stopped and ongoing bleeding has been developed. The morphological substrate of hemorrhagic complications in a new viral infection was studied. All patients were treated with conservative and minimally invasive procedures (embolization, puncture with pressure bandage). 15 patients (57.7%) recovered, 11 patients (42.3%) died from the progression of COVID-19 complications.Conclusion. Comprehensive clinical and laboratory sequential instrumental diagnosis of soft tissue hemorrhages in COVID-19. Treatment should be conservative and significantly invasive. The use of the term βsoft tissue hematomaβ in SARS-CoV-2 infected patients is not a natural quality of the normal pathological process and should not be observed from our point of view.Π¦Π΅Π»Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ: ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ Π²ΠΈΠ·ΡΠ°Π»ΡΠ½ΡΠ΅ ΡΠ»ΡΡΡΠ°Π·Π²ΡΠΊΠΎΠ²ΡΠ΅, ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎ-ΡΠΎΠΌΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈ Π°Π½Π³ΠΈΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΊΡΠΈΡΠ΅ΡΠΈΠΈ ΠΌΡΠ³ΠΊΠΎΡΠΊΠ°Π½Π½ΡΡ
ΠΊΡΠΎΠ²ΠΎΠΈΠ·Π»ΠΈΡΠ½ΠΈΠΉ/Π³Π΅ΠΌΠ°ΡΠΎΠΌ, ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π²ΠΈΠ·ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ Ρ Π±ΠΎΠ»ΡΠ½ΡΡ
, ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
SARS-CoV-2, ΠΈΠ·ΡΡΠΈΡΡ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠΉ Π² ΠΌΡΠ³ΠΊΠΈΡ
ΡΠΊΠ°Π½ΡΡ
, ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΡΡΡΡ ΠΏΠΎΠ½ΡΡΠΈΡ ΠΈ Π²ΡΡΠ°Π±ΠΎΡΠ°ΡΡ Π»Π΅ΡΠ΅Π±Π½ΡΡ ΡΠ°ΠΊΡΠΈΠΊΡ ΠΏΡΠΈ ΡΠ°ΠΊΠΎΠΌ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΈ COVID-19.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ° 4 ΠΌΠ΅Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΠΎΠΆΠΈΠ»ΡΡ
Π±ΠΎΠ»ΡΠ½ΡΡ
(+60), ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
SARS-CoV-2, Π²ΡΡΠ²Π»Π΅Π½ΠΎ 40 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΌΡΠ³ΠΊΠΎΡΠΊΠ°Π½Π½ΡΠΌΠΈ ΠΊΡΠΎΠ²ΠΎΠΈΠ·Π»ΠΈΡΠ½ΠΈΡΠΌΠΈ/Π³Π΅ΠΌΠ°ΡΠΎΠΌΠ°ΠΌΠΈ, ΠΈΠ· Π½ΠΈΡ
26 (65%) ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π±ΠΎΠ»ΡΡΠΈΠΌΠΈ Π³Π΅ΠΌΠ°ΡΠΎΠΌΠ°ΠΌΠΈ (ΡΠ°Π·ΠΌΠ΅Ρ >10 ΡΠΌ ΠΈ ΠΎΠ±ΡΠ΅ΠΌ> 1000 ΠΌΠ»). ΠΡΠΎΠ²Π΅Π΄Π΅Π½ Π°Π½Π°Π»ΠΈΠ· ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ, ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ (Π£ΠΠ β 26 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², ΠΠ’ β 10 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², Π°Π½Π³ΠΈΠΎΠ³ΡΠ°ΡΠΈΡ β 9 Π±ΠΎΠ»ΡΠ½ΡΡ
, ΠΏΡΠ½ΠΊΡΠΈΠΈ β 6 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ²), Π² 11 ΡΠ»ΡΡΠ°ΡΡ
ΠΈΠ·ΡΡΠ΅Π½ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π» Π°ΡΡΠΎΠΏΡΠΈΠΈ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. Π‘ΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠΌΠΈ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΌΠ΅ΡΠΎΠ΄Π°ΠΌΠΈ ΠΏΠΎΠ»ΡΡΠ΅Π½Π° Π²ΠΈΠ·ΡΠ°Π»ΡΠ½Π°Ρ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΊΡΠΎΠ²ΠΎ ΠΈΠ·Π»ΠΈΡΠ½ΠΈΠΉ/Π³Π΅ΠΌΠ°ΡΠΎΠΌ ΠΌΡΠ³ΠΊΠΎΡΠΊΠ°Π½Π½ΠΎΠΉ ΠΏΠ»ΠΎΡΠ½ΠΎΡΡΠΈ, ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π° ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½ΠΎΡΡΡ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π²ΠΈΠ·ΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠΈ. Π Π°Π·ΡΠ°Π±ΠΎΡΠ°Π½Π° ΡΠ°ΠΊΡΠΈΠΊΠ° Π²Π΅Π΄Π΅Π½ΠΈΡ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠ° ΠΏΡΠΈ ΠΎΡΡΠ°Π½ΠΎΠ²ΠΈΠ²ΡΠ΅ΠΌΡΡ ΠΈ ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠ°ΡΡΠ΅ΠΌΡΡ ΠΊΡΠΎΠ²ΠΎΡΠ΅ΡΠ΅Π½ΠΈΠΈ. ΠΠ·ΡΡΠ΅Π½ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΡΡΠ±ΡΡΡΠ°Ρ Π³Π΅ΠΌΠΎΡΡΠ°Π³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΡ ΠΏΡΠΈ Π½ΠΎΠ²ΠΎΠΉ Π²ΠΈΡΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ. ΠΡΠ΅ Π±ΠΎΠ»ΡΠ½ΡΠ΅ ΠΏΡΠΎΠ»Π΅ΡΠ΅Π½Ρ ΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ²Π½ΡΠΌΠΈ ΠΈ ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½ΠΎ ΠΈΠ½Π²Π°Π·ΠΈΠ²Π½ΡΠΌΠΈ ΠΏΡΠΎΡΠ΅Π΄ΡΡΠ°ΠΌΠΈ (ΡΠΌΠ±ΠΎΠ»ΠΈΠ·Π°ΡΠΈΡ, ΠΏΡΠ½ΠΊΡΠΈΡ Ρ Π΄Π°Π²ΡΡΠ΅ΠΉ ΠΏΠΎΠ²ΡΠ·ΠΊΠΎΠΉ). ΠΡΠ·Π΄ΠΎΡΠΎΠ²Π΅Π»ΠΈ 15 (57,7%) ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ², ΡΠΌΠ΅ΡΠ»ΠΈ ΠΎΡ ΠΏΡΠΎΠ³ΡΠ΅ΡΡΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΎΡΠ»ΠΎΠΆΠ½Π΅Π½ΠΈΠΉ COVID-19 11 (42,3%) Π±ΠΎΠ»ΡΠ½ΡΡ
.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½Π°Ρ ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½Π°Ρ ΠΏΠΎΡΠ»Π΅Π΄ΠΎΠ²Π°ΡΠ΅Π»ΡΠ½Π°Ρ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΠ°Π»ΡΠ½Π°Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ° ΠΌΡΠ³ΠΊΠΎΡΠΊΠ°Π½Π½ΡΡ
ΠΊΡΠΎΠ²ΠΎΠΈΠ·Π»ΠΈΡΠ½ΠΈΠΉ ΠΏΡΠΈ COVID-19-ΠΈΠ½ΡΠ΅ΠΊΡΠΈΠΈ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΠ΅Ρ ΡΠ²ΠΎΠ΅Π²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎ ΡΡΡΠ°Π½ΠΎΠ²ΠΈΡΡ ΡΠΎΠΏΠΈΡΠ΅ΡΠΊΠΈΠΉ Π΄ΠΈΠ°Π³Π½ΠΎΠ·, ΠΎΡΠ΅Π½ΠΈΡΡ ΡΡΠΆΠ΅ΡΡΡ ΠΊΡΠΎΠ²ΠΎΠΏΠΎΡΠ΅ΡΠΈ, Π΅Π΅ ΠΎΡΡΠ°Π½ΠΎΠ²ΠΊΡ ΠΈΠ»ΠΈ ΠΏΡΠΎΠ΄ΠΎΠ»ΠΆΠ΅Π½ΠΈΠ΅ ΠΊΡΠΎΠ²ΠΎΡΠ΅ΡΠ΅Π½ΠΈΡ. ΠΠ΅ΡΠ΅Π½ΠΈΠ΅ Π΄ΠΎΠ»ΠΆΠ½ΠΎ Π½ΠΎΡΠΈΡΡ ΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ²Π½ΡΠΉ ΠΈ ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½ΠΎ ΠΈΠ½Π²Π°Π·ΠΈΠ²Π½ΡΠΉ Ρ
Π°ΡΠ°ΠΊΡΠ΅Ρ. ΠΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅ΡΠΌΠΈΠ½Π° βΠ³Π΅ΠΌΠ°ΡΠΎΠΌΠ°β ΠΌΡΠ³ΠΊΠΈΡ
ΡΠΊΠ°Π½Π΅ΠΉ Ρ ΠΈΠ½ΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
SARS-CoV-2 Π±ΠΎΠ»ΡΠ½ΡΡ
Π½Π΅ ΠΎΡΡΠ°ΠΆΠ°Π΅Ρ ΡΡΡΡ ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΡΡΠ΅Π³ΠΎ ΠΏΠ°ΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΏΡΠΎΡΠ΅ΡΡΠ° ΠΈ, Ρ Π½Π°ΡΠ΅ΠΉ ΡΠΎΡΠΊΠΈ Π·ΡΠ΅Π½ΠΈΡ, Π½Π΅ Π΄ΠΎΠ»ΠΆΠ½ΠΎ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΡΡΡ
Effect of oxygen loaded nanodroplets on human endothelial cells and macrophages cultured in normoxic versus hypoxic conditions
Policies and practices of contemporary confinement ENS de Lyon, 20 and 21 June 2016 OurΒ programme (EN) More information: clickΒ ici
Effect of oxygen loaded nanodroplets on human endothelial cells and macrophages cultured in normoxic versus hypoxic conditions
Aminobisphosphonate-activated gamma delta T cells in immunotherapy of cancer: doubts no more
Background: Activated V gamma V iota 2 T cells are able to kill most tumour cells because of recognition by T cell receptor and natural killer receptors. Objective: We discuss the possibility that the intentional activation of gamma delta T cells in vivo by aminobisphosphonates may represent a promising target for the design of novel and highly innovative immunotherapy in cancer patients. Methods: The antitumoural effects of gamma delta T cells both in vitro and in vivo have been demonstrated suggesting a new therapeutic approach for translation into the clinical setting. Results/conclusion: V gamma 9V delta 2 T lymphocytes represent a particularly interesting target for immunotherapeutic protocols based on N-aminobisphosphonate administration and several Phase I-II trials are ongoing investigating the activity of zoledronic acid plus IL-2 in solid tumours