Short communication: Culture of Chlorella ellipsoidea in different inexpensive medium and used as food for production of rotifer, Brachionus calyciflorus

Rotifers are multicellular animals with body cavities that are partially lined by mesoderm. These organisms are valuable live food for larval fish and crustacean culture. Several characteristics of rotifers, including their nutritional quality, body size and relatively slow motility have contributed to their usefulness as good prey for active larvae. In general, rotifers have both nutrient content and a high rate of daily production. ... In the present study, we cultured Chlorella ellipsoidea in different media and used as food in powdered and fresh live form along with Backer’s yeast to mass production of rotifers

Effect of use of duckweed powder as a fish feed on monoculture of silver carp (Hypophthalmichthys molitrix)

An experiment on the use of duckweed powder as a fish feed on monoculture of silver carp (Hypophthalmichthys molitrix) was conducted in 6 ponds for a period of 75 days. The area of the each pond was one decimal. The experiment was carried out under two treatments, each with 3 replications. A combination of duckweed powder and rice bran at the ratio of 3:1 was supplied at the rate of 4% of total body weight in the ponds under treatment-I. On the other hand, the ponds under treatment-II were without supplying of feed. Each of the ponds under both treatments were stocked with 45 fingerlings of silver carp (H. molitrix). The average initial length and weight of the fingerlings were 14.60 cm and 33.48 g, respectively. The ponds were fertilized fortnightly with poultry droppings at the rate of 2 kg, urea 60 g and TSP 90 g decimal-1. During the experimental period the ranges of physico-chemical parameters viz. water depth (0.82 to 0.90 m), water temperature (17.80 to 26.7 °C), air temperature (19.11 to 28.29 °C), transparency (28.00 to 34.00 cm), dissolved oxygen (6.70 to 8.20 mg L-1), pH (6.70 to 8.00), total alkalinity (170 to 210 mg L-1), free CO2 (0.0 to 3.50 mg L-1), phosphate-phosphorus (1.2 to 2.9 mg L-1), and nitrate-nitrogen (3.1 to 4.5 mg L-1) were within the productive limit and more or less similar in all the ponds under treatments I and II. There were 25 genera of phytoplankton under four major groups and 10 genera of zooplankton under three major groups in the experimental ponds. Mean survival rates under treatment-I and treatment-II were 97.78% and 95.56%, respectively. The specific growth rates (SGR % per day) of the fish found under treatment-I and treatment-II were 0.98% and 0.49%. The calculated net production of the ponds under treatment-I was 1.87 ton ha-1 yr-1 and that of the ponds under treatment-II, was 0.74 ton ha-1 yr-1. By 't' test, it was found that the net fish production of treatment-I was significantly (p<0.01) higher than that of treatment-II, and cost return relationship was found that the net profit of treatment-I and treatment-II were more or less similar. Finally, it can be concluded that duckweed powder as an ingredient of fish feed had significant impacts on production of silver carp, which do not consume duckweed as fresh and raw condition. Thus, duckweed powder can be used as feed for most fishes. Int. J. Agril. Res. Innov. & Tech. 9 (1): 73-83, June, 201

Suitability of Chlorella ellipsoidea as food for production of the rotifer Brachionus calyciflorus

Rotifers are valuable live food for larval fish and crustacean in aquaculture. In the present study, we conducted an experiment to evaluate the suitability of Chlorella ellipsoidea as food for production of the rotifer, Brachionus calyciflorus. The experiment was carried out in three treatments using dried powder Chlorella ellipsoidea (T1), fresh live Chlorella ellipsoidea (T2) and Bakers yeast (T3) as food with three replications. For supplying food to rotifers, C. ellipsoidea was cultured sufficiently in different inexpensive culture media before rotifer culture and it was continued up to the end of the rotifer culture experiment. The ranges of environmental factors analyzed were suitable for both C. ellipsoidea and rotifers culture during the experimental period. Maximum cell densities of C. ellipsoidea were recorded in inexpensive pulse bran extract medium during the culture period. The mean population densities (means ± SEM) of B. calyciflorus were significantly (p<0.01) highest in T2 (28.6 ± 4.64 (x103) individuals L-1) compared to T1 (11.6 ± 1.24 (x103) individuals L-1) and T3 (11.4 ± 1.82 (x103) individuals L-1). The findings of present study revealed that fresh cultured C. ellipsoidea was the best food for production of the rotifer, B. calyciflorus. DOI: http://dx.doi.org/10.3329/ijarit.v3i2.17843 Int. J. Agril. Res. Innov. & Tech. 3 (2): 41-48, December, 201

Control of euglenophyte bloom and fish production enhancement using duckweed and lime

Euglenophyte bloom is a common problem in most of the aquaculture ponds in Bangladesh. In the present study we conducted an experiment to control euglenophyte bloom for getting better fish production using duckweed (Lemna minor) and lime. The experiment was carried out using four treatments, i.e., ponds were supplied with duckweed (T1), lime treatment (T2), both duckweed and lime (T3) and without supply of duckweed and lime (T4). Rohu, catla, mrigal, silver carp and silver barb were stocked and their gut contents were analyzed monthly. The ranges of water quality parameters were analyzed within the productive limit during the experimental period. The mean abundance of euglenophyte was significantly highest in T4 (17.62 ± 1.97 × 104 cells/L), followed by T2 (2.96 ± 0.20 × 104 cells/L), T1 (1.94 ± 0.35 × 104 cells/L) and T3 (1.53 ± 0.42 × 104 cells/L). Gut content analysis revealed that considerable amount of euglenophyte were consumed by silver carp and silver barb, but not preferred by rohu, catla and mrigal. The gross yields of fish were 2133.37, 1967.76, 2816.52 and 1725.62 kg/ha/5 months in T1, T2, T3 and T4, respectively. The highest fish production in T3 and lowest fish production in T4 indicated the use of duckweed and lime is economically sustainable for controlling euglenophytes bloom, maintaining water quality and getting higher fish production

Control of euglenophyte bloom and fish production enhancement using duckweed and lime

Euglenophyte bloom is a common problem in most of the aquaculture ponds in Bangladesh. In the present study we conducted an experiment to control euglenophyte bloom for achieving better fish production using duckweed (Lemna minor) and lime. The experiment was carried out using four treatments, i.e., ponds were supplied with duckweed (T1), lime treatment (T2), both duckweed and lime (T3) and without supply of duckweed and lime (T4). Rohu, catla, mrigal, silver carp and silver barb were stocked and their gut contents were analyzed monthly. The ranges of water quality parameters were analyzed within the productive limit during the experimental period. The mean abundance of euglenophyte was significantly highest in T4 (17.62 ± 1.97 x 10^4 cells/L), followed by T2 (2.96 ± 0.20 x 10^4 cells/L), T1 (1.94 ± 0.35 x 10^4 cells/L) and T3 (1.53 ± 0.42 x 10^4 cells/L). Gut content analysis revealed that considerable amounts of euglenophyte were consumed by silver carp and silver barb, but not preferred by rohu, catla and mrigal. The gross yields of fish were 2133.37, 1967.76, 2816.52 and 1725.62 kg/ha/5 months in T1, T2, T3 and T4 respectively. The highest fish production in T3 and lowest fish production in T4 indicated the use of duckweed and lime is economically sustainable for controlling euglenophytes bloom, maintaining water quality and getting higher fish production

Determinants of male participation in reproductive healthcare services: a cross-sectional study

Background The role of male’s participation in reproductive healthcare is now well-recognized. The present study investigated the role of men in some selected reproductive health issues, characterizing their involvement, including factors influencing their participation in reproductive healthcare services. Methods This study was conducted in the working areas of urban and rural implemented by NGOs. The sample-size was determined scientifically. The systematic sampling procedure was used for selecting the sample. The study included 615 men aged 25-45 years. Bivariate analysis was performed between male’s involvement as the dependent variable with several independent variables. Logistic regression analysis was applied to assess the effects of risk factors on the participation of men in reproductive health care services. Results The mean age of the respondents was little over 34 years while their mean years of schooling was 3.7, and their mean monthly income was about Tk 3,400 (US$ 1 = Tk 70) at the time of the study. Rickshaw-pulling and driving was the main occupation of the respondents from the urban while farming were main occupation in the rural area respectively. About two-thirds of the respondents discussed reproductive health issues with their wives and accompanied them to healthcare facilities. The current contraceptive-use rate was 63% among the men who attended the evening clinics. Results of bivariate analysis showed a significant association with education, occupation, income, access to media, and number of living children. Results of logistic regression analysis showed that secondary to higher education level, number of living children, paid employment status, long marital duration, and access to media were important correlates of males’ involvement in reproductive healthcare services. Conclusions The results imply that a greater integration of reproductive healthcare matters with the Millennium Development Goals and increasing perception of men through enrollment in various components of reproductive activities will produce synergistic effects

Classification of multichannel EEG signal by linear discriminant analysis

Motor imagery (MI) related Electroencephalogram (EEG) signal classification is one of the main challenge in designing a brain computer interface (BCI) system. Linear Discriminant Analysis (LDA) has a very low computational requirement which makes it suitable for online BCI system. This paper proposes an advanced and simple classification technique for MI related BCI system. Initially the signal is extracted for different features. The LDA classifier has been used to propose technique to design an MI based BCI. For contrastive comparison other classification techniques have been evaluated by classification accuracy and Cohen's kappa

Classification of multichannel EEG signal by single layer perceptron learning algorithm

Motor imagery (MI) related Electroencephalogram (EEG) signal classification is one of the main challenge in designing a brain computer interface (BCI) system. Single Layer Perceptron Learning (SLPL) algorithm has a very low computational requirement which makes it suitable for online BCI system. This paper proposes an advanced and simple classification technique for MI related BCI system. Initially the signal is extracted for different features. The SLPL classifier has been used to propose technique to design an MI based BCI. For contrastive comparison with other classification techniques have been evaluated by classification accuracy, mutual information and Cohen’s kappa