Povezanost između polimorfizama XRCC1 ARG399GLN i P53 ARG72PRO s rizikom od raka želuca i debeloga crijeva u turskoj populaciji

Gastric cancer is one of the most common cancers of the gastrointestinal system, and its overall fi ve-year survival rate is still 15 % to 20 %, as it can mostly be diagnosed at an advanced stage. On the other hand, although colorectal cancer has a rather good prognosis, mortality is one half that of the incidence. As carcinogenesis is believed to involve reactive radicals that cause DNA adduct formation, impaired repair activity, and weakened tumour suppression, it would help to understand the role of the polymorphisms of nucleotide excision repair enzyme XRCC1 and of tumour suppressor gene p53 in gastric and colorectal cancers. Our study included 94 gastric cancer patients, 96 colorectal cancer patients, and 108 cancer-free individuals as control with the aim to see if there was an association between XRCC1 Arg399Gln and p53 Arg72Pro polymorphisms and cancer susceptibility. DNA was extracted from peripheral blood cells and genotypes were determined using the polymerase chain reaction-restriction fragment length polymorphism. Polymorphism p53 Arg72Pro was not associated with either gastric or colorectal carcinoma, while XRCC1 Arg399Gln was not associated with the increased risk of colorectal cancer. However, XRCC1 homozygous Gln allele at codon 399 was associated with 2.54 times higher risk of gastric cancer.Rak želuca najčešći je oblik karcinoma probavnoga sustava, a ukupno mu je preživljenje i dalje 15 % do 20 %, budući da se većinom dijagnosticira u poodmakloj fazi razvoja. S druge pak strane, premda rak debeloga crijeva ima prilično dobru prognozu, smrtnost je i dalje 50 %. Vjeruje se da je nastanak karcinoma povezan s reaktivnim radikalima koji uzrokuju stvaranje DNA-adukata, onemogućavaju popravak DNA te slabe supresiju tumora. Stoga bi bilo korisno razumjeti ulogu polimorfi zama gena za enzim XRCC1 koji sudjeluje u popravku isjecanjem nukleotida i tumor-supresorskoga gena p53 u nastanku raka želuca i debeloga crijeva. Naše je ispitivanje obuhvatilo 94 bolesnika s rakom želuca, 96 bolesnika s rakom debeloga crijeva te 108 kontrolnhih ispitanika (koji nisu oboljeli od bilo kojeg oblika raka) s ciljem da se utvrdi povezanost između polimorfi zama XRCC1 Arg399Gln i p53 Arg72Pro i sklonosti nastanku raka. DNA je dobiven iz stanica periferne krvi, a genotip utvrđen s pomoću metode lančane reakcije polimerazom - polimorfi zma restrikcijskih fragmenata na osnovi dužine (PCRRLFP). Polimorfi zam p53 Arg72Pro nije se pokazao povezanim s povećanim rizikom od raka želuca ili debeloga crijeva niti je XRCC1 Arg399Gln bio povezan s povećanim rizikom od raka debeloga crijeva, ali je zato rizik od raka želuca u homozigotnih nositelja ovoga polimorfi zma bio 2,54 puta veći

Stability of Endohedral Hydrogen Doped Boron Nitride Nanocages: A Density Functional Theory Study

WOS: 000343955700006In this study, the stabilization energies of the nH(2)@BmNm Complexes (m = 12, 24, 36 48;60) have been determined by exploiting several density functional theory methods, namely B3LYP,PBE1PBE and omega B97X-D. Among these density functional theory methods, omega B97X-D is found to be the most appropriate for the systems involving H-2 doping in boron nitride nanocages; It predicted that the smallest nanocage, has no stable complex and the H-2@B24N24,2H(2)@B36N36, 4H(2)@B48N48 and 7H(2)@B60N60 complexes are the most Stable hydrogen-boron nitride complexes. Accordingly, it is found that the number of hydrogen molecules doped inside the most stable complex of each nanocage quadratically depends on nanocage size. This indicates that as the size of nanocage, as well as, the size of the endohedral cavity increases More stable nH(2)@BmNm Complexes are formed,Scientific Research Projects of Ege UniversityEge University [09/FEN/046]This study is supported Scientific Research Projects of Ege University through the project no 09/FEN/046. The numerical calculations reported in this paper were partially performed at TUBITAK ULAKBIM, High Performance and Grid Computing Center (TRUBA resources)

Computational investigation of hydrogen storage capacity of boron nitride nanocages by newly developed PM7 method

Employing the newly developed PM7 method,we have computationally investigated endohedral hydrogen storage capacities of boron-nitride based nanocages, BmNm (m = 12, 24, 36, 48 and 96). The calculation results revealed that the smallest two boron-nitride nanocages, B12N12 and B24N24, (their gravimetric density of hydrogen storages are 1.35 and 3.05 wt. %) are not suitable for hydrogen doping due to their rather small endohedral cavities. B36N36, whose hydrogen storage density is 4.96 wt. %, may not be very efficient although it has better storage capacity than the smallest two. On the other hand, the gravimetric densities of hydrogen storage inside the B48N48 and B96N96 nanocages are predicted to be 6.6 and 10.6 wt. %, respectively. This high weight percentage of hydrogen storage indicates that B48N48 and B96N96 can be considered as promising hydrogen storage materials. © 2015, Chemical Publishing Co. All rights reserved

Tumor volume estimation by the percentage carcinoma method in uterine cervix carcinoma

OBJECTIVE: To assess the capacity of the percentage carcinoma method to predict tumor volume in cervical carcinoma and generate a mathematical equation for calculation of tumor volume