60 research outputs found
Atrazine mineralization in bulk soil and maize rhizosphere
International audienceAn experiment was carried out in the greenhouse in order to compare atrazine mineralization in bulk soil and maize rhizosphere at different development stages. After 4, 8 and 12 weeks, we have (1) measured the soil microbial biomass C, (2) characterized the C substrate utilization profiles of the culturable microflora, and (3) analyzed atrazine mineralization. Microbial growth was stimulated in planted soil and different C substrate utilization patterns were obtained in bulk and rhizosphere soils during the first 2 months. During this period, laboratory tests for atrazine biodegradation revealed a lower mineralization potential in bulk than in planted soil. Atrazine mineralization was stimulated to a greater extent after atrazine application in the greenhouse but again the presence of plants had a favorable effect. After 12 weeks of cropping, the atrazine mineralization potential decreased in planted soil with or without prior atrazine application
Plasma-enhanced protein patterning in a microfluidic compartmentalized platform for multi-organs-on-chip: A liver-tumor model
A microfluidic technique is presented for micropatterning protein domains and cell cultures within permanently bonded organs-on-chip devices. This method is based on the use of polydimethylsiloxane layers coupled with the plasma ablation technique for selective protein removal. We show how this technique can be employed to generate a multi-organ in vitro model directly within a microscale platform suitable for pharmacokinetic-based drug screening. We miniaturized a liver model based on micropatterned co-cultures in dual-compartment microfluidic devices. The cytotoxic effect of liver-metabolized Tegafur on colon cancer cell line was assessed using two microfluidic devices where microgrooves and valves systems are used to model drug diffusion between culture compartments. The platforms can reproduce the metabolism of Tegafur in the liver, thus killing colon cancer cells. The proposed plasma-enhanced microfluidic protein patterning method thus successfully combines the ability to generate precise cell micropatterning with the intrinsic advantages of microfluidics in cell biology
Influence of pea, lentil and faba bean on soil microbial activities implicated in sulfur and nitrogen mineralization
National audienc
Shifts in the nitrate-reducing community induced by the maize rhizosphere
International audienc
Effect of cropping cycles and repeated herbicide applications on the degradation of diclofop-methyl, bentazone, diuron, isoproturon and pendimethalin in soil
International audienc
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