Evaluation of Trust and Revocation of Malicious Nodes in MANETs

Mobile adhoc network (MANET) is formed by a set of mobile hosts which communicate via radio waves within the transmission range. Manets are wireless communication where nodes can freely move within the network. Manets operate in the absence of base station and central access point. Applications of Manets are different in various areas. Manets are famous because of its self configuring, wireless and dispersed. The main factor in Manet is its security. The features of Manets are actually nontrivial problems to the security design in Manets. Manets are vulnerable to attacks due to the absence of the centralized control, dynamic topologies. Hence it’s very important to protect Manets from various types of attacks and revocate the certificate of the malicious nodes

Identification of NBS-LRR Resistance Gene Analogues (RGA) from Rose (IIHRR13-4) Resistant to Powdery Mildew (Podosphaera pannosa (Wallr.:Fr.) de Bary)

Resistance is the best strategy to manage powdery mildew (Podosphaera pannosa (Wallr.:Fr.) de Bary) of rose. Identification of resistant genes (R genes) from plant species will help in breeding programs. Nucleotide Binding Site - Leucine Rich Repeats (NBS- LRR) is a major class of R gene family in plants. This study reports the identification and molecular characterization of resistance gene analogues from roses maintained at ICAR- Indian Institute of Horticultural Research (IIHR). The powdery mildew resistant line IIHRR13-4 was compared with the susceptible commercial cultivar, konfetti. PCR based approaches with degenerative primers based on different conserved motifs of NBS-LRR were employed to isolate resistance gene analogues (RGAs) from rose. Eleven RGAs (IIHRR13-4R1, IIHRR13-4R2, IIHRR13-4R3, IIHRR13-4R4, IIHRR13-4R5, IIHRR13- 4R6, IIHRR13-4R7, IIHRR13-4R8 IIHRR13-4R9 and IIHRR13-4R10) were identified from powdery mildew resistant germplasm line, IIHRR13-4, based on the sequence and similarity to RGAs from rosaceae family and other crops. The major similarity to rose RGAs reported are from Fragaria vesca, Rosa hybrid cultivar, Prunus and Rosa chinensis. RGAs isolated from IIHRR13-4 belonged to Toll Interleukin Receptor (TIR)-NBS-LRR and Non-TIR-NBS-LRR RGAs (Lecine Zipper (LZ) type). Different motifs of RGAs identified were P-loop, RNBS A, kinase 2, kinase 3a, RNBS-D and GLPL of NBS domain. This study reports the existence of resistance at genetic level in powdery mildew resistant genotype IIHRR13-4. These RGAs will be useful for mapping and characterization of R genes in IIHRR13-4 and breeding for improved powdery mildew resistance in roses

Effectiveness of computer assisted instruction on knowledge of the care after cardiac surgery in PICU among mothers of children under 12 years

A pre-experimental study to assess the effectiveness of computer assisted instruction on knowledge of the care after cardiac surgery in PICU among mothers of children under 12 years in selected hospitals at Chennai. The conceptual framework of the study was developed on the basis of Ludwig Yon Bertalanffy(1968) general system theory model .The study variable was mothers of children under 12 years of age. An experimental research approach with pre – experimental pre and post test design was used to achieve the objectives of the study .The study was conducted in MIOT hospitals at Chennai, with a sample size of 30 mothers of children under 12 years. The samples were selected through non – probability convenient sampling technique. The investigator used a demographic variable performa and with a structured questionnaire on knowledge of the care after cardiac surgery in PICU among mothers of children under 12 year to collect the data. The data collection tools were validated and reliability was established. The data was collected by using a self administered questionnaire method. After the pre test computer assisted instruction was administered to the mothers of children under 12 year and the post test was done after 7 days. The demographic characteristics revealed that most of the participants were in the age group of 26-30 years. It was noted that majority of the participants 27 (90%) had moderate knowledge and 3(10%) had inadequate knowledge, in the pre test where as in the post test none of them had inadequate knowledge and majority of them gained adequate knowledge with the mean score of 86.7 %. It was also noted that there was a significant improvement in the post test mean score which were highly significant at P <0.001 level which indicates the effectiveness of computer assisted instruction. There is a significant association between the demographic variables with the pre test and post test levels of knowledge. iv The results indicated that the computer assisted instruction had significantly improved the level of knowledge of the care after cardiac surgery in PICU among mothers of children under 12 years

A CRITICAL REVIEW ON AVALEHA KALPANA

Avaleha is the most common secondary Kalpana (dosage form) which has been employed in various disorders and this product is gaining popularity due to its easy administration, palatability and longer shelf life. It is a semisolid preparation of herbal drugs prepared in decoction or extracts of different herbs by adding sweetening agents like jaggery, sugar or sugar candy. The component drugs which are used in the preparation of Avaleha are aqueous medium, substrate, Oushada dravyas, lipid medium, additives etc. By analyzing consistency of various Avaleha varies from freely-flowing, paste-like, semisolid and granular, which depends upon the substrate and Oushada churna ratio. Avaleha can be compared to confections because both contains essential ingredients as sugar and water and optional ingredients as edible oils, honey, flours, starches, edible salts etc. Avaleha are intend to provide better drug absorption through the oral cavity along with absorption through villi. The literature related to Avaleha has been surveyed from various Ayurvedic literatures, journals, Ayurvedic Formulary of India etc. and presented briefly here. Here we have discussed the information regarding Avaleha like ingredients, method of preparation, Avaleha paka lakshana, non-conformances related to Avaleha production, comparison between Avaleha and confections and absorption

Identification of heat-tolerant rice genotypes and their molecular characterisation using SSR markers

The effect of high-temperature stress has a critical impact in causing reduced crop yield. The focus of the current investigation is the identification of heat-tolerant rice varieties that can alleviate the effects of stress. Among the ten genotypes evaluated across various parameters such as leaf area, dry weight, photosynthetic rate, stomatal conductance and spikelet fertility, N-22 showed superior characteristics for the grain filling parameters along with CR-Dhan 307. The variety CR-Dhan 307 with significantly higher mean pollen viability (80.23%), spikelet fertility (81.18%) and 1000 grain weight (25.45 gm) can be utilized as a heat-tolerant variety. Other genotypes Ptb-7 and CR-Dhan 202 seemed to have tolerance traits beneficial at the vegetative stage. The genotype Rajalakshmi can be characterised as heat susceptible as it had significantly lower values for all parameters. Polymorphic analysis was carried out to validate SSR markers associated with heat tolerance. The polymorphic information content (PIC) was found to be the highest for RM236 and RM6100. The SSR marker RM6100 has been validated in the current investigation to be associated with heat tolerance. As the PIC value is an indication of the ability of the marker in indicating genetic diversity, the PIC values of the 11 polymorphic markers is useful for identify heat-tolerant genotypes. The genetic diversity analysis was carried out using DendroUPGMA to establish the relationship between the genotypes. The genotypes Ptb-7 and CR-Dhan 204 were thus found to be closely related to the heat-tolerant check variety, N-22 indicating genetically related traits for tolerance to heat

Modified Variational Mode Decomposition for Power Line Interference Removal in ECG Signals

Power line interferences (PLI) occurring at 50/60 Hz can corrupt the biomedical recordings like ECG signals and which leads to an improper diagnosis of disease conditions. Proper interference cancellation techniques are therefore required for the removal of these power line disturbances from biomedical recordings. The non-linear time varying characteristics of biomedical signals make the interference removal a difficult task without compromising the actual signal characteristics. In this paper, a modified variational mode decomposition based approach is proposed for PLI removal from the ECG signals. In this approach, the central frequency of an intrinsic mode function is fixed corresponding to the normalized power line disturbance frequency. The experimental results show that the PLI interference is exactly captured both in magnitude and phase and are removed. The proposed approach is experimented with ECG signal records from MIT-BIH Arrhythmia database and compared with traditional notch filtering

Isolation of a novel soil fungus VT-NSK capable of utilizing the distillery spentwash and synthetic melanoidin – a preliminary report

Soil samples collected from Mc Dowell’s Distillery Ltd, Aleppey, India were screened for potent melanoidin degrading fungus. Seven fungi were obtained in pure culture. Out of the seven isolates obtained, one strain showing highest rate of melanoidin degradation was coded as VT-NSK and was characterized in detail. The isolate VT-NSK was systematically identified by microscopy, phylogeny by molecular techniques like 18 S rRNA gene sequencing followed by Blast analysis. The isolate VT-NSK was further screened for the melanoidin degrading activity on 1% synthetic melanoidin and 10% distillery effluent amended Czapek dox medium. The isolate showed a decolourization zone of 61mm diameter in 1% synthetic melanoidin and 69mm diameter in 10% distillery effluent amended czapek dox medium after 48hours of incubation. 18S r RNA gene sequencing of the isolate showed maximum alignment with Cunninghamella blakesleeana sp belonging to zygomycetes class. The sequence has been deposited in GenBank with Accession number  JN570507

Microsphere-Based Scaffolds Carrying Opposing Gradients of Chondroitin Sulfate and Tricalcium Phosphate

Extracellular matrix (ECM) components, such as chondroitin sulfate (CS) and tricalcium phosphate, serve as raw materials, and thus spatial patterning of these raw materials may be leveraged to mimic the smooth transition of physical, chemical, and mechanical properties at the bone-cartilage interface. We hypothesized that encapsulation of opposing gradients of these raw materials in high molecular weight poly(d,l-lactic-co-glycolic acid) (PLGA) microsphere-based scaffolds would enhance differentiation of rat bone marrow–derived stromal cells. The raw material encapsulation altered the microstructure of the microspheres and also influenced the cellular morphology that depended on the type of material encapsulated. Moreover, the mechanical properties of the raw material encapsulating microsphere-based scaffolds initially relied on the composition of the scaffolds and later on were primarily governed by the degradation of the polymer phase and newly synthesized ECM by the seeded cells. Furthermore, raw materials had a mitogenic effect on the seeded cells and led to increased glycosaminoglycan (GAG), collagen, and calcium content. Interestingly, the initial effects of raw material encapsulation on a per-cell basis might have been overshadowed by medium-regulated environment that appeared to favor osteogenesis. However, it is to be noted that in vivo, differentiation of the cells would be governed by the surrounding native environment. Thus, the results of this study demonstrated the potential of the raw materials in facilitating neo-tissue synthesis in microsphere-based scaffolds and perhaps in combination with bioactive signals, these raw materials may be able to achieve intricate cell differentiation profiles required for regenerating the osteochondral interface