A genetic analysis of aluminium tolerance in cereals

Aluminium (Al) toxicity is a major threat to agricultural production world wide wherever acid soil exists. Wheat and barley, the major food and feed crops, are severely affected and this necessitates investigations that could help to improve the yield by utilising the available genetic diversity for Al tolerance with the aid of several molecular platforms. We investigated the quantitative trait loci (QTL) conferring tolerance to Al toxicity in three different mapping populations of wheat and barley.Using a set of D genome (Ae. tauschii) introgression lines, a major Al tolerance locus was assigned to chromosome arm 4DL, explaining 31% of the phenotypic variation displayed by the population. A second major QTL was mapped to chromosome arm 3BL using a set of doubled haploid progeny lines. This major QTL, QaltCS.ipk-3B, originated from ‘Chinese Spring’ accounted for 49% of the variation in the population. The inheritance for Al tolerance in barley was dissected based on a genetic map constructed with genic markers. QTLs were identified on chromosomes 2H, 3H and 4H. A sequence homology search was used to derive the putative function of the genes linked to the QTL, in order to identify potential candidate genes for Al tolerance. Some of these candidates are implicated in stress/defence responses, in particular, stress signal transduction, transcription regulation factors and cell metabolism

A QTL analysis of aluminium tolerance in Barley, using Gene-Based markers

Barley is more sensitive than the other major cereal crops to aluminium (Al) toxicity. Here, a doubled haploid mapping population was exploited to study the inheritance of Al tolerance in barley. Quantitative trait locus (QTL) mapping was based on a genetic map constructed with genic markers. Al tolerance QTL were identified on chromosomes 2H, 3H and 4H. A sequence homology search was used to derive the putative function of the genes linked to the QTL, in order to identify potential candidate genes for Al tolerance. Some of these candidates are implicated in stress/defence responses, in particular, stress signal transduction, transcription regulation factors and cell metabolism

A QTL analysis of aluminium tolerance in barley, using gene-based markers

Barley is more sensitive than the other major cereal crops to aluminium (Al) toxicity. Here, a doubled haploid mapping population was exploited to study the inheritance of Al tolerance in barley. Quantitative trait locus (QTL) mapping was based on a genetic map constructed with genic markers. Al tolerance QTL were identified on chromosomes 2H, 3H and 4H. A sequence homology search was used to derive the putative function of the genes linked to the QTL, in order to identify potential candidate genes for Al tolerance. Some of these candidates are implicated in stress/defence responses, in particular, stress signal transduction, transcription regulation factors and cell metabolism

A Genetic Analysis of Aluminium Tolerance in Cereals

Aluminium (Al) toxicity is a major threat to agricultural production world wide wherever acid soil exists. Wheat and barley, the major food and feed crops, are severely affected and this necessitates investigations that could help to improve the yield by utilising the available genetic diversity for Al tolerance with the aid of several molecular platforms. We investigated the quantitative trait loci (QTL) conferring tolerance to Al toxicity in three different mapping populations of wheat and barley. Using a set of D genome (Ae. tauschii) introgression lines, a major Al tolerance locus was assigned to chromosome arm 4DL, explaining 31% of the phenotypic variation displayed by the population. A second major QTL was mapped to chromosome arm 3BL using a set of doubled haploid progeny lines. This major QTL, QaltCS.ipk-3B, originated from ‘Chinese Spring’ accounted for 49% of the variation in the population. The inheritance for Al tolerance in barley was dissected based on a genetic map constructed with genic markers. QTLs were identified on chromosomes 2H, 3H and 4H. A sequence homology search was used to derive the putative function of the genes linked to the QTL, in order to identify potential candidate genes for Al tolerance. Some of these candidates are implicated in stress/defence responses, in particular, stress signal transduction, transcription regulation factors and cell metabolism

A Genetic Analysis of Aluminium Tolerance in Cereals

Aluminium (Al) toxicity is a major threat to agricultural production world wide wherever acid soil exists. Wheat and barley, the major food and feed crops, are severely affected and this necessitates investigations that could help to improve the yield by utilising the available genetic diversity for Al tolerance with the aid of several molecular platforms. We investigated the quantitative trait loci (QTL) conferring tolerance to Al toxicity in three different mapping populations of wheat and barley. Using a set of D genome (Ae. tauschii) introgression lines, a major Al tolerance locus was assigned to chromosome arm 4DL, explaining 31% of the phenotypic variation displayed by the population. A second major QTL was mapped to chromosome arm 3BL using a set of doubled haploid progeny lines. This major QTL, QaltCS.ipk-3B, originated from ‘Chinese Spring’ accounted for 49% of the variation in the population. The inheritance for Al tolerance in barley was dissected based on a genetic map constructed with genic markers. QTLs were identified on chromosomes 2H, 3H and 4H. A sequence homology search was used to derive the putative function of the genes linked to the QTL, in order to identify potential candidate genes for Al tolerance. Some of these candidates are implicated in stress/defence responses, in particular, stress signal transduction, transcription regulation factors and cell metabolism

Validation of quantitative trait loci for aluminum tolerance in Chinese wheat landrace FSW

Citation: Dai, J., Bai, G., Zhang, D. et al. Euphytica (2013) 192: 171. https://doi.org/10.1007/s10681-012-0807-9Aluminum (Al) toxicity is one of the major constraints for wheat production in acidic soils worldwide and use of Al-tolerant cultivars is one of the most effective approaches to reduce Al damage in the acidic soils. A Chinese landrace, FSW, shows a high level of tolerance to Al toxicity and a mapping population of recombinant inbred lines (RILs) was developed from a cross between FSW and Al-sensitive US spring wheat cultivar Wheaton to validate the quantitative trait loci (QTL) previously identified in FSW. The mapping population was evaluated for net root growth (NRG) during Al stress in a nutrient solution culture and hematoxylin staining score (HSS) of root tips after Al stress. After 132 simple sequence repeat (SSR) markers from three chromosomes that were previously reported to have the QTLs were analyzed in the population, two QTLs for Al tolerance from FSW were confirmed. The major QTL on chromosome 4DL co-segregated with the Al-activated malate transporter gene (ALMT1), however, sequence analysis of the promoter region (Ups4) of ALMT1 gene indicated that FSW contained a marker allele that is different from the one that was reported to condition Al tolerance in the Brazilian source. Another QTL on chromosome 3BL showed a minor effect on Al tolerance in the population. The two QTLs accounted for about 74.9% of the phenotypic variation for HSS and 72.1% for NRG and demonstrated an epistatic effect for both HSS and NRG. SSR markers closely linked to the QTLs have potential to be used for marker-assisted selection (MAS) to improve Al tolerance in wheat breeding programs