12 research outputs found
Strategies for Multiplexed Electrochemical Sensor Development
Detection of multiple biomarkers for disease diagnosis or treatment monitoring has received a lot of attention due to their potential impact on clinical decision making. Electrochemical biosensors have become one of the preferred detection approaches, due to the simplicity of the accompanying instrumentation. This chapter will explore how electrochemical sensors can be utilized for detection of multiple analytes by integration of sensors into microfluidic microsystems. Some key fabrication technologies for such devices will be presented utilizing polymer microfabrication, paper-based approaches, and the use of printed circuit boards. Next, the use of electrode arrays will be presented along with some commercial platforms, outlining plausible paths towards a successful electrochemical multiplexed sensor. Novel approaches based on microbeads and various labels will then be introduced along with various strategies and technologies utilized to achieve ultrasensitive multiplexed detection
Boron Doped Diamond Paste Electrodes for Microfluidic Paper-Based Analytical Devices
Boron
doped diamond (BDD) electrodes have exemplary electrochemical
properties; however, widespread use of high-quality BDD has previously
been limited by material cost and availability. In the present article,
we report the use of a BDD paste electrode (BDDPE) coupled with microfluidic
paper-based analytical devices (ÎĽPADs) to create a low-cost,
high-performance electrochemical sensor. The BDDPEs are easy to prepare
from a mixture of BDD powder and mineral oil and can be easily stencil-printed
into a variety of electrode geometries. We demonstrate the utility
and applicability of BDDPEs through measurements of biological species
(norepinephrine and serotonin) and heavy metals (Pb and Cd) using
ÎĽPADs. Compared to traditional carbon paste electrodes (CPE),
BDDPEs exhibit a wider potential window, lower capacitive current,
and are able to circumvent the fouling of serotonin. These results
demonstrate the capability of BDDPEs as point-of-care sensors when
coupled with ÎĽPADs