Political economy of planned relocation: A model of action and inaction in government responses

This is the author accepted manuscript. The final version is available from Elsevier via the DOI in this record.Planned relocation has been shown to have significant impacts on the livelihoods and wellbeing of people and communities, whether the resettlement process is inclusive or coercive. For states, planned relocation represents risks to those communities but also to government investments and political legitimacy. Evaluations of relocations commonly focus on the risks and benefits of government interventions while overlooking the consequences of not intervening. Here we develop a conceptual framework to examine the factors that influence government decision-making about whether or not to undertake planned relocation of populations in the context of environmental change. The study examines planned relocation decisions and non-decisions by government agencies in West Bengal in India for communities seeking relocation due to coastal flooding. It focuses on three localities facing river erosion losing significant land areas in small islands and communities where populations recognize the need for public intervention, but where there has been a diversity of responses from the state authorities. Data are derived from interviews with key respondents involved in planning and implementing relocation and with residents affected by those government decisions (n = 26). These data show that government action is explained by a combination of risk aversion within political systems to avoid perceived negative consequences, and a lack of government accountability. The empirical cases demonstrate the uneven application of action and inaction and the consequent uneven distribution of potential outcomes on populations. The study suggests that while there may be a growing demand for planned relocation in places affected by environmental change, its implementation is likely to be uneven, with profound socioeconomic implications for those living in such localities.International Development Research Centr

The endogenous HBZ interactome in ATL leukemic cells reveals an unprecedented complexity of host interacting partners involved in RNA splicing

Adult T-cell leukemia/lymphoma (ATL) is a T-cell lymphoproliferative neoplasm caused by the human T-cell leukemia virus type 1 (HTLV-1). Two viral proteins, Tax-1 and HBZ play important roles in HTLV-1 infectivity and in HTLV-1-associated pathologies by altering key pathways of cell homeostasis. However, the molecular mechanisms through which the two viral proteins, particularly HBZ, induce and/or sustain the oncogenic process are still largely elusive. Previous results suggested that HBZ interaction with nuclear factors may alter cell cycle and cell proliferation. To have a more complete picture of the HBZ interactions, we investigated in detail the endogenous HBZ interactome in leukemic cells by immunoprecipitating the HBZ-interacting complexes of ATL-2 leukemic cells, followed by tandem mass spectrometry analyses. RNA seq analysis was performed to decipher the differential gene expression and splicing modifications related to HTLV-1. Here we compared ATL-2 with MOLT-4, a non HTLV-1 derived leukemic T cell line and further compared with HBZ-induced modifications in an isogenic system composed by Jurkat T cells and stably HBZ transfected Jurkat derivatives. The endogenous HBZ interactome of ATL-2 cells identified 249 interactors covering three main clusters corresponding to protein families mainly involved in mRNA splicing, nonsense-mediated RNA decay (NMD) and JAK-STAT signaling pathway. Here we analyzed in detail the cluster involved in RNA splicing. RNAseq analysis showed that HBZ specifically altered the transcription of many genes, including crucial oncogenes, by affecting different splicing events. Consistently, the two RNA helicases, members of the RNA splicing family, DDX5 and its paralog DDX17, recently shown to be involved in alternative splicing of cellular genes after NF-κB activation by HTLV-1 Tax-1, interacted and partially co-localized with HBZ. For the first time, a complete picture of the endogenous HBZ interactome was elucidated. The wide interaction of HBZ with molecules involved in RNA splicing and the subsequent transcriptome alteration strongly suggests an unprecedented complex role of the viral oncogene in the establishment of the leukemic state

Hindlimb suspension in Wistar rats: Sex-based differences in muscle response

Ground-based animal models have been used extensively to understand the effects of microgravity on various physiological systems. Among them, hindlimb suspension (HLS), developed in 1979 in rats, remains the gold-standard and allows researchers to study the consequences of total unloading of the hind limbs while inducing a cephalic fluid shift. While this model has already brought valuable insights to space biology, few studies have directly compared functional decrements in the muscles of males and females during HLS. We exposed 28 adult Wistar rats (14 males and 14 females) to 14 days of HLS or normal loading (NL) to better assess how sex impacts disuse-induced muscle deconditioning. Females better maintained muscle function during HLS than males, as shown by a more moderate reduction in grip strength at 7 days (males: −37.5 ± 3.1%, females: −22.4 ± 6.5%, compared to baseline), that remains stable during the second week of unloading (males: −53.3 ± 5.7%, females: −22.4 ± 5.5%, compared to day 0) while the males exhibit a steady decrease over time (effect of sex × loading p = 0.0002, effect of sex × time × loading p = 0.0099). This was further supported by analyzing the force production in response to a tetanic stimulus. Further functional analyses using force production were also shown to correspond to sex differences in relative loss of muscle mass and CSA. Moreover, our functional data were supported by histomorphometric analyzes, and we highlighted differences in relative muscle loss and CSA. Specifically, female rats seem to experience a lesser muscle deconditioning during disuse than males thus emphasizing the need for more studies that will assess male and female animals concomitantly to develop tailored, effective countermeasures for all astronauts

Perceived environmental risks and insecurity reduce future migration intentions in hazardous migration source areas

This is the author accepted manuscript. The final version is available from Cell Press via the DOI in this record. Environmental change influences population movements at various temporal and spatial scales. Yet individual decisions to migrate involve multiple motivations including perceived environmental risks and economic opportunities. We analyze how perceptions of environmental risks affect migration decisions and future migration intentions. We use cross-sectional household survey data (N = 5,450) from populations engaged in migration in net out-migration areas in four coastal areas in Ghana, Bangladesh, and India to examine ex post-migration motivations and ex ante-future migration intentions. The data include variables on previous migration, migration intentions, well-being, food insecurity, and perceived long-term environmental degradation. The results show that few households identified environmental risks as the primary driver for past migration decisions. Perceived increased severity of drought and household insecurity both reduce stated future migration intentions. Hence, perceptions of environmental risks, including future potential changes, are significant in altering aggregate migration flows from source areas in low-lying coastal regions.Collaborative Adaptation Research Initiative in Africa and Asia (CARIAA)UK Foreign, Commonwealth and Development OfficeInternational Development Research Centre, Ottawa, CanadaBelmont ForumUK Research and Innovatio

Human T Cell Leukemia Virus Reactivation with Progression of Adult T-Cell Leukemia-Lymphoma

Background: Human T-cell leukemia virus-associated adult T-cell leukemia-lymphoma (ATLL) has a very poor prognosis, despite trials of a variety of different treatment regimens. Virus expression has been reported to be limited or absent when ATLL is diagnosed, and this has suggested that secondary genetic or epigenetic changes are important in disease pathogenesis. Methods and Findings: We prospectively investigated combination chemotherapy followed by antiretroviral therapy for this disorder. Nineteen patients were prospectively enrolled between 2002 and 2006 at five medical centers in a phase II clinical trial of infusional chemotherapy with etoposide, doxorubicin, and vincristine, daily prednisone, and bolus cyclophosphamide (EPOCH) given for two to six cycles until maximal clinical response, and followed by antiviral therapy with daily zidovudine, lamivudine, and alpha interferon-2a for up to one year. Seven patients were on study for less than one month due to progressive disease or chemotherapy toxicity. Eleven patients achieved an objective response with median duration of response of thirteen months, and two complete remissions. During chemotherapy induction, viral RN