Osmopriming combined with Boron-Tolerant Bacteria (Bacillus sp. MN54) improved the productivity of Desi Chickpea under rainfed and irrigated conditions

Chickpeas are rich source of protein and predominantly grown in boron (B)-deficient sandy-loam soils in Pakistan. Boron-tolerant bacteria (BTB) could tolerate higher B levels in soil and increase B availability to the plants. Field trials were conducted under irrigated (district Layyah) and rainfed (district Chakwal) conditions to evaluate the interactive effects of pre-optimized B application methods and BTB (Bacillus sp. MN54) on the nodule’s population, grain quality, productivity, and grain-B concentration in desi chickpea during 2019–2020 and 2020–2021. Boron was applied as soil application (1 kg B ha−1), foliar application (0.025% B), osmopriming (0.001% B), and seed coating (1.5 g B kg−1 seed) with or without BTB inoculation. Untreated seeds receiving no B through any of the methods were regarded as control. The individual and interactive effects (up to three-way interaction of location × BTB inoculation × B application methods) of year, location, B application methods and BTB inoculation significantly altered the growth and yield-related traits of desi chickpea. The four-way interaction of year × location × BTB inoculation × B application methods was non-significant for all recorded growth and yield-related traits. Regarding individual effects, the higher values of growth and yield-related traits were noted for 2020–2021, rainfed location, BTB inoculation and B application through seed priming. Similarly, in two-way interactions 2020–2021 with rainfed location and BTB inoculation, rainfed location with BTB inoculation and osmopriming and osmopriming with BTB inoculation recorded higher values of the growth and yield-related traits. Osmopriming combined with BTB inoculation significantly improved dry matter accumulation and leaf area index in both locations. Boron application through all the methods significantly improved grain quality, yield grain B concentration. The highest grain and biological yields, and nodules’ population were recorded with osmopriming followed by soil application of B combined with BTB inoculation. The highest plant B concentration (75.05%) was recorded with foliar application of B followed by osmopriming (68.73%) combined with BTB inoculation. Moreover, the highest economic returns (USD 2068.5 ha−1) and benefit–cost ratio (3.7%) were recorded with osmopriming + BTB inoculation in 2020–2021 under rainfed conditions. Overall, B application through osmopriming and soil application combined with BTB inoculation could be used to increase productivity and profitability of desi chickpea, whereas foliar application is a better method to enhance grain and plant B concentration

Clinical evaluation of cases of lower genitourinary tract trauma with special reference to primary realignment in cases of posterior urethral distraction defect

Background: The exact management strategy for lower genitourinary tract trauma remains controversial. Primary realignment with/without suprapubic catheterization provides definitive procedure with low complications and avoids the need for further open surgeries.Methods: This was a prospective longitudinal study done on 31 cases with different complaints related to lower tract genitourinary trauma. All patients underwent suprapubic catheterization and/or primary realignment. The outcome was measured in the terms of time for discharge, urinary incontinence, stricture formation, erectile dysfunction and impotence.Results: Maximum proportion of patients with lower genitourinary injuries in the study was in 10-20 years age group (48.4%). Blunt trauma was accounted for 93.6% of lower genitourinary injuries. Road traffic accidents were the most common cause (90.32%) of lower genitourinary injuries. Urinary bladder injuries accounted for 41.9% of all lower genitourinary injuries. Blood at meatus is present in only about half of the significant urethral injuries. Primary realignment of urethral injury results in lesser duration of hospital stay (9.24±2.44 days), shorter length of suprapubic catheterization (11.67±4.78 days) and early spontaneous voiding (40.93±15.79 days). The stricture rate following primary realignment is low (31.25%). Erectile dysfunction was noted only in two patients (16.6%).Conclusions: Management of traumatic urethral disruption by primary realignment serves as ultimate therapy in majority of patients

Soil-Applied boron combined with Boron-Tolerant Bacteria (Bacillus sp. MN54) improve root proliferation and nodulation, yield and agronomic grain biofortification of chickpea (Cicer arietinum L.)

Chickpea is widely cultivated on calcareous sandy soils in arid and semi-arid regions of Pakistan; however, widespread boron (B) deficiencies in these soils significantly decreases its productivity. Soil application of B could improve chickpea yield and grain-B concentration. However, optimizing suitable B level is necessary due to a narrow deficiency and toxicity range of B. Nonetheless, the co-application of B-tolerant bacteria (BTB) and synthetic B fertilizer could be helpful in obtaining higher chickpea yields and grain-B concentration. Therefore, this study optimized the level of soil applied B along with BTB, (i.e., Bacillus sp. MN54) to improve growth, yield and grain-B concentrations of chickpea. The B concentrations included in the study were 0.00 (control), 0.25, 0.50, 0.75 and 1.00 mg B kg−1 soil combined with or without Bacillus sp. MN54 inoculation. Soil application of B significantly improved root system, nodulation, yield and grain-B concentration, and Bacillus sp. MN54 inoculation further improved these traits. Moreover, B application at a lower dose (0.25 mg B kg−1 soil) with BTB inoculation recorded the highest improvements in root system (longer roots with more roots’ proliferation), growth, nodulation and grain yield. However, the highest grain-B concentration was recorded under a higher B level (0.75 mg B kg−1 soil) included in the study. Soil application of 0.25 mg B kg−1 with Bacillus sp. MN54 inoculation improved growth and yield-related traits, especially nodule population (81%), number of pods plant−1 (38%), number of grains plant−1 (65%) and grain yield (47%) compared with control treatment. However, the grain-B concentration was higher under the highest B level (1.00 mg kg−1 soil) with Bacillus sp. MN54 inoculation. In conclusion, soil application of 0.25 mg B kg−1 with Bacillus sp. MN54 inoculation is a pragmatic option to improve the root system, nodule population, seedling growth, yield and agronomic grain-B biofortification of chickpea

A novel design, analysis and 3D printing of Ti-6Al-4V alloy bio-inspired porous femoral stem

The current study is proposing a design envelope for porous Ti-6Al-4V alloy femoral stems to survive under fatigue loads. Numerical computational analysis of these stems with a body-centered-cube (BCC) structure is conducted in ABAQUS. Femoral stems without shell and with various outer dense shell thicknesses (0.5, 1.0, 1.5, and 2 mm) and inner cores (porosities of 90, 77, 63, 47, 30, and 18%) are analyzed. A design space (envelope) is derived by using stem stiffnesses close to that of the femur bone, maximum fatigue stresses of 0.3?ys in the porous part, and endurance limits of the dense part of the stems. The Soderberg approach is successfully employed to compute the factor of safety Nf > 1.1. Fully porous stems without dense shells are concluded to fail under fatigue load. It is thus safe to use the porous stems with a shell thickness of 1.5 and 2 mm for all porosities (18-90%), 1 mm shell with 18 and 30% porosities, and 0.5 mm shell with 18% porosity. The reduction in stress shielding was achieved by 28%. Porous stems incorporated BCC structures with dense shells and beads were successfully printed. [Figure not available: see fulltext.] 2020, The Author(s).This paper was made possible by NPRP grant# NPRP 8-876-2-375 from the Qatar National Research Fund (a member of Qatar Foundation), and seed project # PSU-23-12-2019. The findings achieved herein are solely the responsibility of the authors. The authors would also like to thank Structures and Materials Research Lab at Prince Sultan University. Open Access funding provided by the Qatar National Library.Scopu

Night vision obstacle detection and avoidance based on Bio-Inspired Vision Sensors

Moving towards autonomy, unmanned vehicles rely heavily on state-of-the-art collision avoidance systems (CAS). However, the detection of obstacles especially during night-time is still a challenging task since the lighting conditions are not sufficient for traditional cameras to function properly. Therefore, we exploit the powerful attributes of event-based cameras to perform obstacle detection in low lighting conditions. Event cameras trigger events asynchronously at high output temporal rate with high dynamic range of up to 120 $dB$. The algorithm filters background activity noise and extracts objects using robust Hough transform technique. The depth of each detected object is computed by triangulating 2D features extracted utilising LC-Harris. Finally, asynchronous adaptive collision avoidance (AACA) algorithm is applied for effective avoidance. Qualitative evaluation is compared using event-camera and traditional camera.Comment: Accepted to IEEE SENSORS 202

Synthesis and Characterization of Silver-Coated Polymeric Scaffolds for Bone Tissue Engineering: Antibacterial and In Vitro Evaluation of Cytotoxicity and Biocompatibility

In bone tissue engineering, multifunctional composite materials are very challenging. Bone tissue engineering is an innovative technique to develop biocompatible scaffolds with suitable orthopedic applications with enhanced antibacterial and mechanical properties. This research introduces a polymeric nanocomposite scaffold based on arabinoxylan-co-acrylic acid, nano-hydroxyapatite (nHAp), nano-aluminum oxide (nAl₂O₃), and graphene oxide (GO) by free-radical polymerization for the development of porous scaffolds using the freeze-drying technique. These polymeric nanocomposite scaffolds were coated with silver (Ag) nanoparticles to improve antibacterial activities. Together, nHAp, nAl₂O₃, and GO enhance the multifunctional properties of materials, which regulate their physicochemical and biomechanical properties. Results revealed that the Ag-coated polymeric nanocomposite scaffolds had excellent antibacterial properties and better microstructural properties. Regulated morphological properties and maximal antibacterial inhibition zones were found in the porous scaffolds with the increasing amount of GO. Moreover, the nanosystem and the polymeric matrix have improved the compressive strength (18.89 MPa) and Young’s modulus (198.61 MPa) of scaffolds upon increasing the amount of GO. The biological activities of the scaffolds were investigated against the mouse preosteoblast cell lines (MC3T3-E1) and increasing the quantities of GO helps cell adherence and proliferation. Therefore, our findings showed that these silver-coated polymeric nanocomposite scaffolds have the potential for engineering bone tissue

What can motivate Lady Health Workers in Pakistan to engage more actively in tuberculosis case-finding?

BACKGROUND: Many interventions to motivate community health workers to perform better rely on financial incentives, even though it is not clear that monetary gain is the main motivational driver. In Pakistan, Lady Health Workers (LHW) are responsible for delivering community level primary healthcare, focusing on rural and urban slum populations. There is interest in introducing large-scale interventions to motivate LHW to be more actively involved in improving tuberculosis case-finding, which is low in Pakistan. METHODS: Our study investigated how to most effectively motivate LHW to engage more actively in tuberculosis case-finding. The study was embedded within a pilot intervention that provided financial and other incentives to LHW who refer the highest number of tuberculosis cases in three districts in Sindh province. We conducted semi-structured interviews with 20 LHW and 12 health programme managers and analysed these using a framework categorising internal and external sources of motivation. RESULTS: Internal drivers of motivation, such as religious rewards and social recognition, were salient in our study setting. While monetary gain was identified as a motivator by all interviewees, programme managers expressed concerns about financial sustainability, and LHW indicated that financial incentives were less important than other sources of motivation. LHW emphasised that they typically used financial incentives provided to cover patient transport costs to health facilities, and therefore financial incentives were usually not perceived as rewards for their performance. CONCLUSIONS: This study indicated that interventions in addition to, or instead of, financial incentives could be used to increase LHW engagement in tuberculosis case-finding. Our finding about the strong role of internal motivation (intrinsic, religious) in Pakistan suggests that developing context-specific strategies that tap into internal motivation could allow infectious disease control programmes to improve engagement of community health workers without being dependent on funding for financial incentives

‘We need to share our stories’: the lives of Pakistanis with intellectual disability and their guardians

© 2020 MENCAP and International Association of the Scientific Study of Intellectual and Developmental Disabilities and John Wiley & Sons Ltd Introduction: The experiences of Pakistanis with intellectual disabilities (IDs) and their family members have been underexplored empirically. Method: The present study sought to address this gap by understanding the lives of five Special Olympics Pakistan athletes and their guardians through PhotoVoice. Findings: Through thematic analysis, we present the primary theme concerning Pakistan\u27s cultural context that provides an empirical exploration of cultural beliefs about intellectual disability, cultural expectations and support received by people with intellectual disabilities and their guardians. Discussion: We discuss implications for research and practice