An Isoform of the Eukaryotic Translation Elongation Factor 1A (eEF1a) Acts as a Pro-Viral Factor Required for Tomato Spotted Wilt Virus Disease in <i>Nicotiana benthamiana</i>

The tripartite genome of the negative-stranded RNA virus Tomato spotted wilt orthotospovirus (TSWV) is assembled, together with two viral proteins, the nucleocapsid protein and the RNA-dependent RNA polymerase, into infectious ribonucleoprotein complexes (RNPs). These two viral proteins are, together, essential for viral replication and transcription, yet our knowledge on the host factors supporting these two processes remains limited. To fill this knowledge gap, the protein composition of viral RNPs collected from TSWV-infected Nicotiana benthamiana plants, and of those collected from a reconstituted TSWV replicon system in the yeast Saccharomyces cerevisiae, was analysed. RNPs obtained from infected plant material were enriched for plant proteins implicated in (i) sugar and phosphate transport and (ii) responses to cellular stress. In contrast, the yeast-derived viral RNPs primarily contained proteins implicated in RNA processing and ribosome biogenesis. The latter suggests that, in yeast, the translational machinery is recruited to these viral RNPs. To examine whether one of these cellular proteins is important for a TSWV infection, the corresponding N. benthamiana genes were targeted for virus-induced gene silencing, and these plants were subsequently challenged with TSWV. This approach revealed four host factors that are important for systemic spread of TSWV and disease symptom development

Telemedicine Improves Performance of a Two-Incision Lower Leg Fasciotomy by Combat Medics:A Randomized Controlled Trial

Introduction:The primary aim of this randomized controlled trial was to assess if a head-mounted display (HMD) providing telemedicine support improves performance of a two-incision lower leg fasciotomy by a NATO special operations combat medic (combat medic).Materials and Methods:Thirty-six combat medics were randomized into two groups: One group performed a two-incision lower leg fasciotomy with the assistance of an HMD, while the control group completed the procedure without guidance. A Mann-Whitney U test was used to determine the possible differences in release of compartments and performance scores, as assessed by a supervising medical specialist. A Fisher's exact test was used to compare the proportions of collateral damage between groups. An independent-samples t-test was used to interpret total procedure times. The usability and technical factors involving HMD utilization were also assessed.Results:Combat medics in the HMD group released the anterior compartment (P &lt;= .001) and deep posterior compartment (P = .008) significantly better. There was significantly more iatrogenic muscle (P &lt;= .001) and venous damage (P &lt;= .001) in the control group. The overall performance of combat medics in the HMD group was significantly better than that of the control group (P &lt; .001). Combat medics in the control group were significantly faster (P = .012). The combat medics were very satisfied with the HMD. The HMD showed no major technical errors.Conclusions:This randomized controlled trial shows that a HMD providing telemedicine support leads to significantly better performance of a two-incision lower leg fasciotomy by a combat medic with less iatrogenic muscle and venous damage

Telemedicine Improves Performance of a Two-Incision Lower Leg Fasciotomy by Combat Medics:A Randomized Controlled Trial

Introduction:The primary aim of this randomized controlled trial was to assess if a head-mounted display (HMD) providing telemedicine support improves performance of a two-incision lower leg fasciotomy by a NATO special operations combat medic (combat medic).Materials and Methods:Thirty-six combat medics were randomized into two groups: One group performed a two-incision lower leg fasciotomy with the assistance of an HMD, while the control group completed the procedure without guidance. A Mann-Whitney U test was used to determine the possible differences in release of compartments and performance scores, as assessed by a supervising medical specialist. A Fisher's exact test was used to compare the proportions of collateral damage between groups. An independent-samples t-test was used to interpret total procedure times. The usability and technical factors involving HMD utilization were also assessed.Results:Combat medics in the HMD group released the anterior compartment (P &lt;= .001) and deep posterior compartment (P = .008) significantly better. There was significantly more iatrogenic muscle (P &lt;= .001) and venous damage (P &lt;= .001) in the control group. The overall performance of combat medics in the HMD group was significantly better than that of the control group (P &lt; .001). Combat medics in the control group were significantly faster (P = .012). The combat medics were very satisfied with the HMD. The HMD showed no major technical errors.Conclusions:This randomized controlled trial shows that a HMD providing telemedicine support leads to significantly better performance of a two-incision lower leg fasciotomy by a combat medic with less iatrogenic muscle and venous damage

Toward the PSTN/Internet Inter-Networking--Pre-PINT Implementations

This document contains the information relevant to the development of the inter-networking interfaces underway in the Public Switched Telephone Network (PSTN)/Internet Inter-Networking (PINT) Working Group. It addresses technologies, architectures, and several (but by no means all) existing pre-PINT implementations of the arrangements through which Internet applications can request and enrich PSTN telecommunications services. The common denominator of the enriched services (a.k.a. PINT services) is that they combine the Internet and PSTN services in such a way that the Internet is used for non-voice interactions, while the voice (and fax) are carried entirely over the PSTN. One key observation is that the pre-PINT implementations, being developed independently, do not inter-operate. It is a task of the PINT Working Group to define the inter-networking interfaces that will support inter-operation of the future implementations of PINT services

The Cool ISM in S0 Galaxies. I. A Survey of Molecular Gas

Lenticular galaxies remain remarkably mysterious as a class. Observations to date have not led to any broad consensus about their origins, properties and evolution, though they are often thought to have formed in one big burst of star formation early in the history of the Universe, and to have evolved relatively passively since then. In that picture, current theory predicts that stellar evolution returns substantial quantities of gas to the interstellar medium; most is ejected from the galaxy, but significant amounts of cool gas might be retained. Past searches for that material, though, have provided unclear results. We present results from a survey of molecular gas in a volume-limited sample of field S0 galaxies, selected from the Nearby Galaxies Catalog. CO emission is detected from 78 percent of the sample galaxies. We find that the molecular gas is almost always located inside the central few kiloparses of a lenticular galaxy, meaning that in general it is more centrally concentrated than in spirals. We combine our data with HI observations from the literature to determine the total masses of cool and cold gas. Curiously, we find that, across a wide range of luminosity, the most gas rich galaxies have about 10 percent of the total amount of gas ever returned by their stars. That result is difficult to understand within the context of either monolithic or hierarchical models of evolution of the interstellar medium.Comment: 26 pages of text, 15 pages of tables, 10 figures. Accepted for publication in the Astrophysical Journa

A Flux-limited Sample of Bright Clusters of Galaxies from the Southern Part of the ROSAT All-Sky Survey: the Catalog and the LogN-LogS

We describe the selection of an X-ray flux-limited sample of bright clusters of galaxies in the southern hemisphere, based on the first analysis of the ROSAT All-Sky Survey data (RASS1). The sample is constructed starting from an identification of candidate clusters in RASS1, and their X-ray fluxes are remeasured using the Steepness Ratio Technique. This method is better suited than the RASS1 standard algorithm for measuring flux from extended sources. The final sample is count-rate-limited in the ROSAT hard band (0.5-2.0 keV), so that due to the distribution of NH, its effective flux limit varies between about 3-4 x 10**-12 ergs cm**-2 s**-1 over the selected area. This covers the Decl<2.5 deg part of the south Galactic cap region (b<-20 deg) - with the exclusion of patches of low RASS1 exposure time and of the Magellanic Clouds area - for a total of 8235 deg**2. 130 candidate sources fulfill our selection criteria for bonafide clusters of galaxies in this area. Of these, 101 are Abell/ACO clusters, while 29 do not have a counterpart in these catalogs. Of these clusters, 126 (97%) clusters have a redshift and for these we compute an X-ray luminosity. 20% of the cluster redshifts come from new observations, as part of the ESO Key Program REFLEX Cluster Survey that is under completion. Considering the intrinsic biases and incompletenesses introduced by the flux selection and source identification processes, we estimate the overall completeness to be better than 90%. The observed number count distribution, LogN-LogS, is well fitted by a power law with slope alpha = 1.34 +/- 0.15 and normalization A = 11.87 +/- 1.04 sr**-1 (10**-11 ergs cm**-2 s**-1)**alpha, in good agreement with other measurements.Comment: 27 pages, 8 figures and 3 tables included, LaTex, emulateapj.sty and epsf.sty, accepted for publication in ApJ: scheduled for the March 20, 1999, Vol.514. The cluster catalog is available at http://www.merate.mi.astro.it/~degrand

Harmonization of the intracellular cytokine staining assay

Active immunotherapy for cancer is an accepted treatment modality aiming to reinforce the T-cell response to cancer. T-cell reactivity is measured by various assays and used to guide the clinical development of immunotherapeutics. However, data obtained across different institutions may vary substantially making comparative conclusions difficult. The Cancer Immunotherapy Immunoguiding Program organizes proficiency panels to identify key parameters influencing the outcome of commonly used T-cell assays followed by harmonization. Our successes with IFNγ-ELISPOT and peptide HLA multimer analysis have led to the current study on intracellular cytokine staining (ICS). We report the results of three successive panels evaluating this assay. At the beginning, 3 out of 9 participants (33 %) were able to detect >6 out of 8 known virus-specific T-cell responses in peripheral blood of healthy individuals. This increased to 50 % of the laboratories in the second phase. The reported percentages of cytokine-producing T cells by the different laboratories were highly variable with coefficients of variation well over 60 %. Variability could partially be explained by protocol-related differences in background cytokine production leading to sub-optimal signal-to-noise ratios. The large number of protocol variables prohibited identification of prime guidelines to harmonize the assays. In addition, the gating strategy used to identify reactive T cells had a major impact on assay outcome. Subsequent harmonization of the gating strategy considerably reduced the variability within the group of participants. In conclusion, we propose that first basic guidelines should be applied for gating in ICS experiments before harmonizing assay protocol variables