Bayesian space-time analysis of health insurance data

Generalized linear models (GLMs) and semiparametric extensions provide a flexible framework for analyzing the claims process in non-life insurance. Currently, most applications are still based on traditional GLMs, where covariate effects are modelled in form of a linear predictor. However, these models may already be too restrictive if nonlinear effects of metrical covariates are present. Moreover, although data are often collected within longer time periods and come from different geographical regions, effects of space and time are usually totally neglected. We provide a Bayesian semiparametric approach, which allows to simultaneously incorporate effects of space, time and further covariates within a joint model. The method is applied to analyze costs of hospital treatment and accommodation for a large data set from a German health insurance company

Ethylene receptor ETR2 controls trichome branching by regulating microtubule assembly in Arabidopsis thaliana

The single-celled trichome of Arabidopsis thaliana is a widely used model system for studying cell development. While the pathways that control the later stages of trichome development are well characterized, the early signalling events that co-ordinate these pathways are less well understood. Hormones such as gibberellic acid, salicylic acid, cytokinins, and ethylene are known to affect trichome initiation and development. To understand the role of the plant hormone ethylene in trichome development, an Arabidopsis loss-of-function ethylene receptor mutant, etr2-3, which has completely unbranched trichomes, is analysed in this study. It was hypothesized that ETR2 might affect the assembly of the microtubule cytoskeleton based on analysis of the cytoskeleton in developing trichomes, and exposures to paclitaxol and oryzalin, which respectively act either to stabilize or depolymerize the cytoskeleton. Through epistatic and gene expression analyses it is shown that ETR2 is positioned upstream of CHROMATIN ASSEMBLY FACTOR1 and TRYPTICHON and is independent of the GLABRA2 and GLABRA3 pathways. These results help extend understanding of the early events that control trichome development and identify a signalling pathway through which ethylene affects trichome branching

Plasmodesmal receptor-like kinases identified through analysis of rice cell wall extracted proteins

In plants, plasmodesmata (PD) are intercellular channels that function in both metabolite exchange and the transport of proteins and RNAs. Currently, many of the PD structural and regulatory components remain to be elucidated. Receptor-like kinases (RLKs) belonging to a notably expanded protein family in plants compared to the animal kingdom have been shown to play important roles in plant growth, development, pathogen resistance, and cell death. In this study, cell biological approaches were used to identify potential PD-associated RLK proteins among proteins contained within cell walls isolated from rice callus cultured cells. A total of 15 rice RLKs were investigated to determine their subcellular localization, using an Agrobacterium-mediated transient expression system. Of these six PD-associated RLKs were identified based on their co-localization with a viral movement protein that served as a PD marker, plasmolysis experiments, and subcellular localization at points of wall contact between spongy mesophyll cells. These findings suggest potential PD functions in apoplasmic signaling in response to environmental stimuli and developmental inputs

A Case Study of Children\u27s Literacy Development in a Voluntary Pre-Kindergarten Classroom

This article describes a year-long study, involving three teachers and 13 children (nine boys and four girls), that examined the impact of instructional practices on student achievement in a voluntary pre-kindergarten (VPK) class. A variety of data were collected: standardized measures, observations, and interviews. The qualitative data revealed several themes that were characteristic of the teachers, as well as the district mandates that affected the teacher, curriculum, and students. The quantitative analysis revealed the limited impact of the VPK program on the students\u27 literacy development and achievement