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In vivo and in vitro models of demyelinating diseases. V. Comparison of the assembly of mouse hepatitis virus, strain JHM, in two murine cell lines.
The developmental sequence of a neurotropic strain (JHM) of mouse hepatitis virus was examined by transmission electron microscopy and immunocytology. The nucleoprotein core of this coronavirus, which contains RNA of positive polarity and is helical in configuration, becomes incorporated into enveloped particles in the same manner as the nucleocapsids of the orthomyxo- and paramyxoviruses. However, JHM virus is assembled intracellularly by budding at surfaces of smooth membranous vacuoles. A comparison of JHM virus replication in L2 and 17Cl-1 cell lines revealed that L2 cells undergo more rapid cytopathology and cease virus production much sooner than 17Cl-l cells. In L2 cells the accumulation of core material appears to continue after the abrupt cessation of virus assembly. This is evident by the massive cytoplasmic accumulation of structure resembling nucleocapsids, which react with hybridoma antibody to the nucleocapsid antigen as demonstrated by the immunoperoxidase procedure. The current findings are consistent with our previously published demonstration, using cells of neural and other deviation, of the fundamental role of the host cell type in regulating the replication and expression of coronaviruses
Boojums and the Shapes of Domains in Monolayer Films
Domains in Langmuir monolayers support a texture that is the two-dimensional
version of the feature known as a boojum. Such a texture has a quantifiable
effect on the shape of the domain with which it is associated. The most
noticeable consequence is a cusp-like feature on the domain boundary. We report
the results of an experimental and theoretical investigation of the shape of a
domain in a Langmuir monolayer. A further aspect of the investigation is the
study of the shape of a ``bubble'' of gas-like phase in such a monolayer. This
structure supports a texture having the form of an inverse boojum. The
distortion of a bubble resulting from this texture is also studied. The
correspondence between theory and experiment, while not perfect, indicates that
a qualitative understanding of the relationship between textures and domain
shapes has been achieved.Comment: replaced with published version, 10 pages, 13 figures include
Folding Langmuir Monolayers
The maximum pressure a two-dimensional surfactant monolayer is able to
withstand is limited by the collapse instability towards formation of
three-dimensional material. We propose a new description for reversible
collapse based on a mathematical analogy between the formation of folds in
surfactant monolayers and the formation of Griffith Cracks in solid plates
under stress. The description, which is tested in a combined microscopy and
rheology study of the collapse of a single-phase Langmuir monolayer of
2-hydroxy-tetracosanoic acid (2-OH TCA), provides a connection between the
in-plane rheology of LM's and reversible folding
Theory of monolayers with boundaries: Exact results and Perturbative analysis
Domains and bubbles in tilted phases of Langmuir monolayers contain a class
of textures knows as boojums. The boundaries of such domains and bubbles may
display either cusp-like features or indentations. We derive analytic
expressions for the textures within domains and surrounding bubbles, and for
the shapes of the boundaries of these regions. The derivation is perturbative
in the deviation of the bounding curve from a circle. This method is not
expected to be accurate when the boundary suffers large distortions, but it
does provide important clues with regard to the influence of various energetic
terms on the order-parameter texture and the shape of the domain or bubble
bounding curve. We also look into the effects of thermal fluctuations, which
include a sample-size-dependent effective line tension.Comment: replaced with published version, 21 pages, 16 figures include
Avoided Critical Behavior in O(n) Systems
Long-range frustrating interactions, even if their strength is infinitesimal,
can give rise to a dramatic proliferations of ground or near-ground states. As
a consequence, the ordering temperature can exhibit a discontinuous drop as a
function of the frustration. A simple model of the doped Mott insulator, where
the short-range tendency of the holes to phase separate competes with
long-range Coulomb effects, exhibits this "avoided critical" behavior. This
model may serve as a paradigm for many other systems.Comment: 4 pages, 2 figure
Polar Smectic Films
We report on a new experimental procedure for forming and studying polar
smectic liquid crystal films. A free standing smectic film is put in contact
with a liquid drop, so that the film has one liquid crystal/liquid interface
and one liquid crystal/air interface. This polar environment results in changes
in the textures observed in the film, including a boojum texture and a
previously unobserved spiral texture in which the winding direction of the
spiral reverses at a finite radius from its center. Some aspects of these
textures are explained by the presence of a Ksb term in the bulk elastic free
energy density that favors a combination of splay and bend deformations.Comment: 4 pages, REVTeX, 3 figures, submitted to PR
Reversible Random Sequential Adsorption of Dimers on a Triangular Lattice
We report on simulations of reversible random sequential adsorption of dimers
on three different lattices: a one-dimensional lattice, a two-dimensional
triangular lattice, and a two-dimensional triangular lattice with the nearest
neighbors excluded. In addition to the adsorption of particles at a rate K+, we
allow particles to leave the surface at a rate K-. The results from the
one-dimensional lattice model agree with previous results for the continuous
parking lot model. In particular, the long-time behavior is dominated by
collective events involving two particles. We were able to directly confirm the
importance of two-particle events in the simple two-dimensional triangular
lattice. For the two-dimensional triangular lattice with the nearest neighbors
excluded, the observed dynamics are consistent with this picture. The
two-dimensional simulations were motivated by measurements of Ca++ binding to
Langmuir monolayers. The two cases were chosen to model the effects of changing
pH in the experimental system.Comment: 9 pages, 10 figure
Towards a Tetravalent Chemistry of Colloids
We propose coating spherical particles or droplets with anisotropic
nano-sized objects to allow micron-scale colloids to link or functionalize with
a four-fold valence, similar to the sp3 hybridized chemical bonds associated
with, e.g., carbon, silicon and germanium. Candidates for such coatings include
triblock copolymers, gemini lipids, metallic or semiconducting nanorods and
conventional liquid crystal compounds. We estimate the size of the relevant
nematic Frank constants, discuss how to obtain other valences and analyze the
thermal distortions of ground state configurations of defects on the sphere.Comment: Replaced to improve figures. 4 figures Nano Letter
A Phase-Field Model of Spiral Dendritic Growth
Domains of condensed-phase monolayers of chiral molecules exhibit a variety
of interesting nonequilibrium structures when formed via pressurization. To
model these domain patterns, we add a complex field describing the tilt degree
of freedom to an (anisotropic) complex-phase-field solidification model. The
resulting formalism allows for the inclusion of (in general, non-reflection
symmetric) interactions between the tilt, the solid-liquid interface, and the
bond orientation. Simulations demonstrate the ability of the model to exhibit
spiral dendritic growth.Comment: text plus Four postscript figure file
Interface dynamics for layered structures
We investigate dynamics of large scale and slow deformations of layered
structures. Starting from the respective model equations for a non-conserved
system, a conserved system and a binary fluid, we derive the interface
equations which are a coupled set of equations for deformations of the
boundaries of each domain. A further reduction of the degrees of freedom is
possible for a non-conserved system such that internal motion of each domain is
adiabatically eliminated. The resulting equation of motion contains only the
displacement of the center of gravity of domains, which is equivalent to the
phase variable of a periodic structure. Thus our formulation automatically
includes the phase dynamics of layered structures. In a conserved system and a
binary fluid, however, the internal motion of domains turns out to be a slow
variable in the long wavelength limit because of concentration conservation.
Therefore a reduced description only involving the phase variable is not
generally justified.Comment: 16 pages; Latex; revtex aps; one figure. Revision: screened coulomb
interaction with coulomb limi
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