<i>In vitro </i>Shoot Regeneration from Leaf and Nodal Explants of <i>Enicostemma</i> <i>hyssopifolium </i>(Willd.) Verd.— A Vulnerable Medicinal Plant

401-404Enicostemma hyssopifolium (Willd.) Verd. plants were established in aseptic cultures from surface sterilized leaf and single node stem segments. Multiple shoots were elicited from leaf explants on MS medium supplemented with BAP (1.5 mg1-1) and IAA (0.5 mg 1-1), while from nodal explants on BAP (1.0 mg 1-1) and IAA (0.5 mg1-1). Maximum shoot proliferation and elongation was established in shoots derived from leaf explants on MS medium supplemented with KN (1.0 mg 1-1) and BAP (1.0 mg 1-1), while in shoots derived from nodal explant it was on MS supplemented with KN (1.0 mg 1-1) and BAP (0.5 mg 1-1). Plantlets were rooted on 1/2 strength MS medium supplemented with IAA (1.0 mg 1-1). The in vitro raised plantlets were acclimatized in green-house and successfully transplanted to the nursery

High frequency shoot regeneration from <i style="">Phyllanthus amarus</i> Schum. & Thonn.

103-107Shoot tip, nodal and internodal segments of Phyllanthus amarus Schum. & Thonn. were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyl amino purine (BAP) and kinetin (Kn) with 15% coconut milk. All the explants responded for regeneration. High frequency (100%) and maximum number of shoots (18.30 ± 0.47) were recorded on medium containing BAP (0.5 mg/l) in shoot tip culture. In vitro shoots were excised from shoot clumps and transferred to rooting medium containing different concentrations of indole butyric acid (IBA) or naphthalene acetic acid (NAA). Maximum number of roots was induced in medium containing IBA (0.5 mg/l). Rooted plantlets were hardened on MS basal liquid medium and subsequently in sterile soil + vermiculite (1:1). Plantlets thus developed were successfully established and finally transferred to greenhouse. The survival rate of the plantlets in the field was found to be very high (85%)