12 research outputs found
Generation of HSC-Like Cells from Human Embryonic Stem Cells by Inhibition of TGF-βR2 Signaling
Get PDFDifferentiation of Mouse Ovarian Stem Cells Toward Oocyte-Like Structure by Coculture with Granulosa Cells
No full textFemale Fertility Preservation through Stem Cell-based Ovarian Tissue Reconstitution In Vitro and Ovarian Regeneration In Vivo
No full textEvaluating the Mechanical Behavior of Basalt Fibers/Epoxy Composites Containing Surface-modified CaCO3 Nanoparticles
No full textThe effect of surface modified nanoclay on the interfacial and mechanical properties of basalt fiber metal laminates
No full textMouse oocyte vitrification: the effects of two methods on maturing germinal vesicle breakdown oocytes
Get PDFPurpose Evaluation of viability and subsequent developmental
ability of mouse germinal vesicle breakdown
oocytes vitrified in conventional straws.
Methods Oocytes with compact cumulus cells were cultured
for 3 h in TCM199 medium GVBD and vitrified by
two methods: the step-wise and single-step. After vitrification,
the oocytes were thawed, and subjected to in vitro
maturation and in vitro fertilization. Oocyte survival (postthaw)
was assessed by morphological appearance and
staining, using propidium iodide (PI)/Hoechst 33342. The
oocyte maturation and fertilization rates were examined in
vitro.
Results In the single-step method the rates of post thaw
survival, maturation to metaphase II and cleavage (2-cell
embryos) were 58.68%, 56.41% and 38.63%, respectively.
In the step-wise method, the corresponding rates were
81.75%, 68.59% and 51.80%, respectively.
Conclusion Vitrification of mouse germinal vesicle breakdown
oocytes by the step-wise method had the advantag
Spermidine induces cytoprotective autophagy of female germline stem cells in vitro and ameliorates aging caused by oxidative stress through upregulated sequestosome-1/p62 expression
No full text
Human GV oocytes generated by mitotically active germ cells obtained from follicular aspirates
No full textFACS-sorted putative oogonial stem cells from the ovary are neither DDX4-positive nor germ cells
No full text
