Synthesis, spectral characterisation and pharmacological studies on Co(II), Ni(II), Cu(II) and Zn(II) bis-Schiff base complexes derived from 4-hydroxybenzohydrazide

915-926The transition metal(II) chelates have been prepared by the reactions of bis-Schiff base ligands derived from 4-hydroxy benzohydrazide and isophthalaldehyde/ o-phthaldehyde in 1:1 metal to ligand ratio. The formed ligands and their metal complexes have been investigated by elemental analysis, different spectroscopic and thermal analyses techniques. The low molar conductance values give the non-electrolytic nature of the metal(II) chelates. From the spectroscopic observations of complexes, the molecular formula is found to be [M(Ln)2Cl2] (where M= Co(II), Ni(II), Cu(II) and Zn(II), n=1 and 2) and the tetradentate N2O2 donor sites of ligands is attached to the metal centre. The docking results predicts that the protein with ligands have good interaction energy. All formed complexes have efficient antibacterial activity than ligand for tested pathogens. The mode of binding interaction of ct-DNA and Cu(II) complexes gives the intercalative binding with hypochromism shifts using electronic titrations method. In electrophoresis, cleavages of PUC18DNA to cleave effectively with Cu(II) complexes in the presence of hydroxyl radicals. The antioxidant assay of DPPH radical scavenging activity of tested compounds shows good results, when compared to ascorbic acid as standard. The anticancer MTT assay of Cu(II) complexes tested against MCF-7 cancerous cells gives the promising therapeutic activity

Flash Spark Plasma Sintering (FSPS) of Pure ZrB2

Export Date: 19 August 2014 CODEN: JACTA Correspondence Address: Reece, M.J.; School of Engineering and Material Science, Queen Mary University of London, London E1 4NS, United Kingdom; email: [email protected] Funding Details: EP/K008749/1, EPSRC, European Commission Funding Details: FP7 2007-2013, EC, European Commission References: Cologna, M., Rashkova, B., Raj, R., Flash Sintering of Nanograin Zirconia in <5 s at 850°C (2010) J. Am. Ceram. Soc., 93 (11), pp. 3556-3559; Downs, J.A., Sglavo, V.M., Electric Field Assisted Sintering of Cubic Zirconia at 390°C (2013) J. Am. Ceram. Soc., 96 (5), pp. 1342-1344; Muccillo, R., Muccillo, E.N.S., An Experimental Setup for Shrinkage Evaluation during Electric Field-Assisted Flash Sintering: Application to Yttria-Stabilized Zirconia (2013) J. Eur. Ceram. Soc., 33 (3), pp. 515-520; Muccillo, R., Muccillo, E.N.S., Electric Field-Assisted Flash Sintering of Tin Dioxide (2014) J. Eur. Ceram. Soc., 34 (4), pp. 915-923; Jha, S.K., Raj, R., The Effect of Electric Field on Sintering and Electrical Conductivity of Titania (2014) J. Am. Ceram. Soc., 97 (2), pp. 527-534; Zapata-Solvas, E., Bonilla, S., Wilshaw, P.R., Todd, R.I., Preliminary Investigation of Flash Sintering of SiC (2013) J. Eur. Ceram. Soc., 33 (1314), pp. 2811-2816; Grasso, S., Sakka, Y., Rendtorff, N., Hu, C., Maizza, G., Borodianska, H., Vasylkiv, O., Modeling of the Temperature Distribution of flash sintered Zirconia (2011) Nippon Seramikkusu Kyokai Gakujutsu Ronbunshi/J. Ceram. Soc. Jpn., 119 (1386), pp. 144-146; Park, J., Chen, I.W., In Situ Thermometry Measuring Temperature Flashes Exceeding 1,700°C in 8 mol% Y2O3-Stablized Zirconia under Constant-Voltage Heating (2013) J. Am. Ceram. Soc., 96 (3), pp. 697-700; Zapata-Solvas, E., Jayaseelan, D.D., Lin, H.T., Brown, P., Lee, W.E., Mechanical Properties of ZrB2- and HfB2-Based Ultra-High Temperature Ceramics Fabricated by Spark Plasma Sintering (2013) J. Eur. Ceram. Soc., 33 (7), pp. 1373-1386; Grasso, S., Sakka, Y., Maizza, G., Electric Current Activated/Assisted Sintering (ECAS): A Review of Patents 1906-2008 (2009) Sci. Technol. Adv. Mater., 10 (5), p. 053001; Mallik, M., Kailath, A.J., Ray, K.K., Mitra, R., Electrical and Thermophysical Properties of ZrB2 and HfB 2 Based Composites (2012) J. Eur. Ceram. Soc., 32 (10), pp. 2545-2555; Steil, M.C., Marinha, D., Aman, Y., Gomes, J.R.C., Kleitz, M., From Conventional Ac Flash-Sintering of YSZ to Hyper-Flash and Double Flash (2013) J. Eur. Ceram. Soc., 33 (11), pp. 2093-2101; Ortiz, A.L., Zamora, V., Rodríguez-Rojas, F., A Study of the Oxidation of ZrB2 Powders during High-Energy Ball-Milling in Air (2012) Ceram. Int., 38 (4), pp. 2857-2863; Porwal, H., Tatarko, P., Grasso, S., Hu, C., Boccaccini, A.R., Dlouhý, I., Reece, M., Toughened and Machinable Glass Matrix Composites Reinforced with Graphene and Graphene-Oxide Nano Platelets (2013) Sci. Technol. Adv. Mater., 14, p. 055007 Pure ZrB2 powder was Flash sintered in an SPS furnace (FSPS). The samples were densified up to 95.0% in 35 s under an applied pressure of 16 MPa. Compared to Conventional SPS (CSPS), the newly developed FSPS technique resulted in an unprecedented energy and time savings of about 95% and 98% respectively. ZrB2 monoliths obtained by CSPS and FSPS were compared with respect to microstructures, densification behavior, and grain growth. The developed methodology might find application to a wide range of highly conductive ceramics as such refractory borides and carbides. © 2014 The American Ceramic Society.Export Date: 19 August 2014 CODEN: JACTA Correspondence Address: Reece, M.J.; School of Engineering and Material Science, Queen Mary University of London, London E1 4NS, United Kingdom; email: [email protected] Funding Details: EP/K008749/1, EPSRC, European Commission Funding Details: FP7 2007-2013, EC, European Commission References: Cologna, M., Rashkova, B., Raj, R., Flash Sintering of Nanograin Zirconia in <5 s at 850°C (2010) J. Am. Ceram. Soc., 93 (11), pp. 3556-3559; Downs, J.A., Sglavo, V.M., Electric Field Assisted Sintering of Cubic Zirconia at 390°C (2013) J. Am. Ceram. Soc., 96 (5), pp. 1342-1344; Muccillo, R., Muccillo, E.N.S., An Experimental Setup for Shrinkage Evaluation during Electric Field-Assisted Flash Sintering: Application to Yttria-Stabilized Zirconia (2013) J. Eur. Ceram. Soc., 33 (3), pp. 515-520; Muccillo, R., Muccillo, E.N.S., Electric Field-Assisted Flash Sintering of Tin Dioxide (2014) J. Eur. Ceram. Soc., 34 (4), pp. 915-923; Jha, S.K., Raj, R., The Effect of Electric Field on Sintering and Electrical Conductivity of Titania (2014) J. Am. Ceram. Soc., 97 (2), pp. 527-534; Zapata-Solvas, E., Bonilla, S., Wilshaw, P.R., Todd, R.I., Preliminary Investigation of Flash Sintering of SiC (2013) J. Eur. Ceram. Soc., 33 (1314), pp. 2811-2816; Grasso, S., Sakka, Y., Rendtorff, N., Hu, C., Maizza, G., Borodianska, H., Vasylkiv, O., Modeling of the Temperature Distribution of flash sintered Zirconia (2011) Nippon Seramikkusu Kyokai Gakujutsu Ronbunshi/J. Ceram. Soc. Jpn., 119 (1386), pp. 144-146; Park, J., Chen, I.W., In Situ Thermometry Measuring Temperature Flashes Exceeding 1,700°C in 8 mol% Y2O3-Stablized Zirconia under Constant-Voltage Heating (2013) J. Am. Ceram. Soc., 96 (3), pp. 697-700; Zapata-Solvas, E., Jayaseelan, D.D., Lin, H.T., Brown, P., Lee, W.E., Mechanical Properties of ZrB2- and HfB2-Based Ultra-High Temperature Ceramics Fabricated by Spark Plasma Sintering (2013) J. Eur. Ceram. Soc., 33 (7), pp. 1373-1386; Grasso, S., Sakka, Y., Maizza, G., Electric Current Activated/Assisted Sintering (ECAS): A Review of Patents 1906-2008 (2009) Sci. Technol. Adv. Mater., 10 (5), p. 053001; Mallik, M., Kailath, A.J., Ray, K.K., Mitra, R., Electrical and Thermophysical Properties of ZrB2 and HfB 2 Based Composites (2012) J. Eur. Ceram. Soc., 32 (10), pp. 2545-2555; Steil, M.C., Marinha, D., Aman, Y., Gomes, J.R.C., Kleitz, M., From Conventional Ac Flash-Sintering of YSZ to Hyper-Flash and Double Flash (2013) J. Eur. Ceram. Soc., 33 (11), pp. 2093-2101; Ortiz, A.L., Zamora, V., Rodríguez-Rojas, F., A Study of the Oxidation of ZrB2 Powders during High-Energy Ball-Milling in Air (2012) Ceram. Int., 38 (4), pp. 2857-2863; Porwal, H., Tatarko, P., Grasso, S., Hu, C., Boccaccini, A.R., Dlouhý, I., Reece, M., Toughened and Machinable Glass Matrix Composites Reinforced with Graphene and Graphene-Oxide Nano Platelets (2013) Sci. Technol. Adv. Mater., 14, p. 055007 Pure ZrB2 powder was Flash sintered in an SPS furnace (FSPS). The samples were densified up to 95.0% in 35 s under an applied pressure of 16 MPa. Compared to Conventional SPS (CSPS), the newly developed FSPS technique resulted in an unprecedented energy and time savings of about 95% and 98% respectively. ZrB2 monoliths obtained by CSPS and FSPS were compared with respect to microstructures, densification behavior, and grain growth. The developed methodology might find application to a wide range of highly conductive ceramics as such refractory borides and carbides. © 2014 The American Ceramic Society.S.G. was supported by EPSRC (EP/K008749/1, XMat). T.S. was supported by EC FP7 2007-2013 (ADMACOM). O.C. was supported by CONACYT (Consejo Nacional de Ciencia y Tecnología, México)

Biochemical characterization and 16s rRNA sequencing of different bacteria from textile dye effluents

Environmental pollution has been identified as a major problem in the modern world. Dyeing effluents have become a vital source of water pollution. Release of coloured textile effluents is undesirable in the aquatic environment as they reduce light penetration, thereby affecting aquatic life and limits utilization of the water media. In Tirupur, the textile factories discharge millions of litres of untreated effluents into the drains that eventually empty into river, Noyyal. The release of coloured compound into water bodies is undesirable not only because of their impact on photosynthesis of aquatic plants but also due to the carcinogenic nature of these dyes and their breakdown products. The ability of bacterial strains isolated from the dye effluent of textile mill sites. Morphological and biochemical characterization was done to identify isolates and was found to be Pseudomonas spp, Bacillus spp and Serattia spp. The isolated strains were finally identified by 16S rRNA sequence analysis. Bacteria are generally identified by 16S rRNA sequencing. The rRNA is the most conserved (least variable) gene in all cells. They were identified as Pseudomonas aeruginosa, Bacillus amyloliquefaciens&nbsp;and Serattia liquefaciens. The sequences were deposited in GENBANK. The accession numbers were KU041528, KU041530 and KU041531 respectively. The identification was conformed by 16S rRNA sequencing. Keywords: Textile Dye Effluents, Bacteria, 16S rRNA, NCBI

A Study on Establishment of Phytochemical Analysis of Quality Parameters and Fluorescence Analysis of Costus spicatus- rhizome extract Medicinal Plants a Well Known Tropical Folklore Medicine

Objective: To evaluate the diagnostic pharmacognostical characters of Costus spicatus in rhizomes extract along with their physico-chemical parameters and fluorescence analysis. Methods: The pharmacognostical characters were determined in terms of macroscopy, microscopy, Powder microscopy, rhizome constant, fluorescence analysis and Preliminary phytochemical investigation. Result: The fluorescence characteristics of rhizome powdered extracts were determined under UV radiation of long and short wavelengths and ordinary visible light. When the powdered drug and extracts were treated with different reagents and observed under UV and ordinary light, they emitted various colour radiations. The colour change for the rhizome powder and individual extract were distinctive and reproducible revealing the solvent properties to the phytoconstituents and data is present. The moister content was calculated through the loss on drying method and was found to be 1.43%. The swelling and foaming index values were recorded. The phytochemical investigation indicates the presence of the organic phytoconstituents such as alkaloids, carbohydrate, flavonoids, protein, amino acids, phenols, tannin, glycosides, steroids and inorganic constituents like iron, phosphate, sulphate and chlorides. Conclusion: Various pharmacognostical and physicochemical parameters have pivotal roles in identification, authentication and establishment of quality parameters of the species. Keywords: Costus spicatus, Physico-Chemical parameters and fluorescence analysis

Phytochemical screening and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Silybum marianum (L).

Objective: To investigate the phytochemicals and GC-MS analysis of ethanol extracts of Silybum marianum. Methods: The air-dried leaves were powdered and subjected to selective sequential extraction using solvents of increasing polarity through percolation, ethanol to obtain an ethanolic extract. Then, each of the extracts was further subjected to gas chromatography-mass spectrometry. Results: Qualitative determination of the different biologically active compounds from crude extracts of Silybum marianum using gas chromatography-mass spectrometry revealed different types of high and low molecular weight chemical entities with varying amounts present in each of the extracts. These chemical compounds are considered biologically and pharmacologically important. Conclusions: The study established the chemical composition and anticancer activity of the plant. Keywords: Silybum marianum, Phytochemicals screening, GC–MS analysis, Bioactive compounds

Phytochemicals and GC–MS analysis of bioactive compounds present in ethanolic leaves extract of Taraxacum officinale (L).

Objective: To identify the phytochemical constituents present in ethanolic leaves extract of Taraxacum officinale (T. o) and to elucidate the bioactive compounds. Methods: The fresh leaves of T. officinale (1000g) were shade dried at room temperature for 30 days and the dried leaves were made into a fine powder. The ethanolic leaves extract obtained was dried with help of desiccator and rotatory evaporator. The dried samples tested for phytochemical analysis and bioactive compounds profiling through GC-MS analysis. Results: The phytochemical screening studies showed presence of alkaloids, flavonoids, phenol, cardiac glycosides and anthraquinones. in the leaves extract of T. officinale. GC-MS analysis showed 10 essential bioactive compounds and it revealed the presence of significant anticancer, Antimicrobial&nbsp; and antidiabetic compounds. Conclusions: The ethanol extract of T. o possess the most important phytochemical compounds and it has various&nbsp; medicinal&nbsp; properties to cure dangerous diseases and disorders. Keywords: Taraxacum officinale, Phytochemicals analysis, GC–MS analysis, Biological compounds

Detection of HF and VHF Fields through Floquet Sideband Gaps by `Rabi Matching' Dressed Rydberg Atoms

Radio frequencies in the HF and VHF (3 MHz to 300 MHz) bands are challenging for Rydberg atom-based detection schemes, as resonant detection requires exciting the atoms to extremely high energy states. We demonstrate a method for detecting and measuring radio frequency (RF) carriers in the HF and VHF bands via a controlled Autler-Townes line splitting. Using a resonant, high-frequency (GHz) RF field, the absorption signal from Townes-Merrit sidebands created by a low frequency, non-resonant RF field can be enhanced. Notably, this technique uses a measurement of the optical frequency separation of an avoided crossing to determine the amplitude of a non-resonant, low frequency RF field. This technique also provides frequency-selective measurements of low frequency RF electric fields. To show this, we demonstrate amplitude modulated signal transduction on a low frequency VHF carrier. We further demonstrate reception of multiple tones simultaneously, creating a Rydberg `spectrum analyzer' over the VHF range.Comment: Data for figures can be found at: https://datapub.nist.gov/od/id/mds2-285