Genetic Differentiation, Mating Systems and Crossability of Three Floral Variants of Sandalwood (Santalum album L.) in Gunung Sewu Geopark, Indonesia

Despite the degradation in their origin in the south-eastern islands of Indonesia, sandalwood (Santalum album L.) recently occurs as new landraces in Gunung Sewu Geopark, Java island, Indonesia. All of the landraces consisted of three floral variants (YBF, refers to ‘yellow big flower’; RBF, ‘red big flower’; and RSF, ‘red small flower,’ respectively), which were differed in floral structures. Isoenzymes and hand-pollination treatments were combined to analyze whether these variants have different genetic diversity, mating systems, and crossing ability. Observed heterozygosity varied significantly with sites (

Paternity Analysis of Tea (Camellia sinensis L. Kuntz) Hybrids Using Isozyme Marker

Tea plant has been categorized as self-incompatible crop. This is the reason behind the high genetic diversity. Natural pollination is possible to occur and the male parent is usually unknown, therefore, there is a need of method to identify male parent of hybrids through paternity analysis. Isozyme markers have been successfully used for paternity analysis due to their co-dominant polymorphism. This research aimed to predict male parents of hybrids by figuring out the mating system through isozyme banding patterns. In this experiment, seven enzyme systems were evaluated, of which only two of the enzyme systems i.e. esterase and shikimate dehydrogenase showing clear band pattern of Est-1, Est-2, and Shd-1 loci. The mating system of tea could be categorized as a mixed mating model, with high estimated out-crossing rate of 98.6 %. The pollen contributors were not always originated from the vicinity of the female parents.Key words: isozyme markers, paternity analysis, te

Interaksi Famili × Lokasi pada Uji Keturunan Generasi Kedua Eucalyptus Pellita

The interaction assessment of genotype and environment is necessary to find out an effective selection strategy in progeny trial, particularly in advanced generations. This study aims to observe the effect of family × site interaction in second generation progeny trial of Eucalyptus pellita planted at two locations: Wonogiri, Central Java and Pelaihari, South Kalimantan. The trials design were completely randomized block design (CRBD) with 49 families, 6-10 blocks, 5 tree-plot and 4 m × 1.5 m spacing. Data measurements included height, diameter at breast height, and stem volume at two and four years age. The study results showed that the families had significant differences (p<0.01) for all measured traits at two and four years age, but the family and site interaction was not statistically significant. Family heritability across the two sites were moderate to high for all traits with the type B genetic correlation ranged from 0.67 to 0.94. Heritability and genetic correlation tended to be higher as tree getting older. Less than 16% of families showed an interactive rank changes in the two locations based on multiple-traits selection index. In general, family selection by combining data from the two sites of progeny trial provided higher genetic gain than those by indirect selection, but it was varied to those direct selection on each site. Therefore, these results imply that, the strategy for family selection by combining data from the two sites of progeny trials should be practiced in appropriate combination and direction by considering the magnitude of genetic variation

Pengaruh Seleksi Terhadap Perolehan Genetik Pada Uji Keturunan Generasi Pertama (F-1) Jabon Merah (Anthocephalus Macrophyllus (Roxb.) Havil) Di Wonogiri

Selection is a fundamental process in the establishment of a progeny trial that will be converted into a seedling seed orchard. In present study, selection practiced in first-generation progeny trial of jabon merah (Anthocephalus macrophyllus Roxb. Havil.) was observed. The study was aimed to find out a criteria selection accompanied with the effective coefficient weight for predicting genetic gain in the first-generation progeny trial of jabon merah. The trial was established in Wonogiri which was laid out in randomized complete block design (RCBD) using 55 families, 4 replications, 4 tree-plot with a spacing of 4 x 2 meter. Measurement was conducted at 40 months after planting covering tree height, diameter at breast height, stem straightness, stem volume. First within-plot selection by culling 2 poorer trees within each plot was practiced at 46 months age. Selection differential from within-plot selection was used to derive coefficient weight which was then applied to predict genetic gain. Results of study showed that selection differential from first within-plot selection were positive for all measured traits. Stem volume was observed as highest priority trait with coefficient weight for each trait were -0.0863 (height), 0.1426 (diameter), 0.2195 (stem straightness), 2.3025 (stem volume). Prediction of genetic gain from within-plot selection were 3,50% (height), 3.58% (diamater), 3.41% (stem straightness), 7.40% (stem volume). While the gain from family selection were 2.80%, 3.75%, 3.54%, 7.10% for height, diamater, stem straightness, stem volume, respectively

Parameter Genetik Sifat Pertumbuhan Dan Kerapatan Kayu Klon Eucalyptus Pellita F. Muell. Di Dua Tapak Yang Berbeda Di Kalimantan Timur

Industrial forest plantations have an important role in fulfilling the wood demand. Based on global industrial development, the forest plantations industry will increase in the following years. Eucalyptus pellita has become main species in Indonesia forest plantations because it has a short cycle and wood products are suitable to forest industry. The average productivity of E. pellita plantations in Indonesia is still low and high variation. In an effort to increase the productivity, the first step is a better understanding of genetic control on growth and basic density. This study aims to determine the genetic parameters for growth and basic density of E. pellita clones on two different sites. The clonal trials was conducted at two different sites. Both clonal trial are designed using RCBD. The number of clones tested in both trial was 30, 5 blocks and 25 tree/plot. The result of the study showed that the effects of clones vary greatly according the enviromental conditions. The clones- environemntal interaction of growth trait is higher than the basic density. This is in line with genetic parameters of growth trait that are less stable than the basic density. The expected genetic gain of growth trait is higher than the basic density and at the same time there was a weak genetic correlation (there is even a negative) between growth trait and basic density. Therefore carefulness is needed in selecting clones when the two traits are used as selection parameters

Variasi Pertumbuhan Tinggi Klon Hibrid Acacia (a. Mangium X a. Auriculiformis) Umur 12 Bulan Di Wonogiri, Jawa Tengah

Forty four clones of Acacia hybrid propagated from selected stoolplants were tested together with a control of pure species seedling and clone in a clonal test established at Wonogiri, Central Java. The design of clonal test was Incomplete Block Design, which was laid-out as single treeplot of 20 replications with spacing of 3 m x 3 m. Measurements of tree height were conducted at age 12 months. There were significant differences between the clones in height growth. Eleven clones of 44 were more superior than the original population at SSO F1 (Control 4th) which superiority ranging from 1.81-35.35 % and 3 of them were more superior than the pure species (control 1th, control 2th and control 3th) which superiority ranging from 3.51 – 17.28%. Meanhile, clone repeatability for height at age 12 months was, while individual repeatability for height was 0.59 +- 0.

Effect of Selection on Genetic Gain of First Generation Seedling Seed Orchard of Jabon Merah (Anthocephalus Macrophyllus Roxb. Havil.) Established in Wonogiri

Selection is a fundamental process in the establishment of a progeny trial that will be converted into a seedling seed orchard. In present study, selection practiced in first-generation progeny trial of jabon merah (Anthocephalus macrophyllus Roxb. Havil.) was observed. The study was aimed to find out a criteria selection accompanied with the effective coefficient weight for predicting genetic gain in the first-generation progeny trial of jabon merah. The trial was established in Wonogiri which was laid out in randomized complete block design (RCBD) using 55 families, 4 replications, 4 tree-plot with a spacing of 4 x 2 meter. Measurement was conducted at 40 months after planting covering tree height, diameter at breast height, stem straightness, stem volume. First within-plot selection by culling 2 poorer trees within each plot was practiced at 46 months age. Selection differential from within-plot selection was used to derive coefficient weight which was then applied to predict genetic gain. Results of study showed that selection differential from first within-plot selection were positive for all measured traits. Stem volume was observed as highest priority trait with coefficient weight for each trait were -0.0863 (height), 0.1426 (diameter), 0.2195 (stem straightness), 2.3025 (stem volume). Prediction of genetic gain from within-plot selection were 3,50% (height), 3.58% (diamater), 3.41% (stem straightness), 7.40% (stem volume). While the gain from family selection were 2.80%, 3.75%, 3.54%, 7.10% for height, diamater, stem straightness, stem volume, respectively