Effects of saline irrigation on growth, physiology and quality of Mesembryanthemum crystallinum L., a rare vegetable crop.

World wide increased desertification due to recent global changes enhances the need of irrigation, which, in turn, provokes the risk of soil salinization. Furthermore, limited fresh water resources may increasingly constrain the use of low quality irrigation water. Hence, intensified use of halotolerant crop plants will be necessary, even in Europe.Commercial use of halophytes as fresh food is limited. Several facultative halophytic members of Aizoaceae are nowadays used as special crop plants. A rare leafy vegetable species is the common ice plant Mesembryanthemum crystallinum, a Crassulacean acid metabolism (CAM) species, which is mostly cultivated in India, California, Australia, and New Zealand. It is also known in Europe as a quickly cooked tender vegetable. With their succulent, mellow, slightly salty tasting leaves and young shoots, M. crystallinum is getting interesting as delicious cool flavored salad greens during recent years. However, it is a perishable product and thus, shelf live is short. On the other hand, CAM capacity of M. crystallinum can be largely enhanced by saline irrigation. Increased CAM potentially reduces water and carbon losses.In this project we studied whether moderate salt treatment affects physiology, growth and yield of this rare crop plant. Furthermore, we investigated whether such treatment that enhances the irreversible C3 to CAM shift in young leaves of this CAM species, potentially prolongs shelf live. Results showed that moderate salt treatment did not negatively influence growth, yield and sensory quality. When in CAM, leaves showed reduced transpiration water losses and CAM also reduced carbon losses during storage

Impact of postharvest UV-C and ozone treatments on microbiological properties of white asparagus (Asparagus officinalis L.)

To meet the increasing demand for safe and high quality fresh white asparagus and the recent food safety regulations, optimization of postharvest handling, processing and storage is essential. Modern sanitation techniques relying on physical methods and/or Generally Recognized As Safe (GRAS) compounds are desired for reducing microbiological spoilage. To evaluate the effects of aqueous ozone and UV-C on the microbial load of spears, samples were UV-C irradiated (254 nm, 1 kJ m-2) and/or washed with ozonated water (approx. 3 ppm or 4.5 ppm at 10 °C), and analyzed at three times during a four day storage. Also, the potential effects of initial natural microbial loads, and precondition of the spears in terms of water and sugar contents on the responsiveness of asparagus to these treatments were determined in detail over four growing seasons. The initial microbial loads (mould and yeasts, and aerobic mesophilic total bacterial counts) of white asparagus spears varied considerably during the different harvest seasons of this four-year study. This variability could not be explained by the variance of climatic conditions nor by the respective water and sugar content. Furthermore, there was never a clear cut relation of the initial microbial load and the growth of pathogens during four-day storage at 20 °C in nearly water vapour saturated atmosphere. Neither washing the spears with ozonated water (3 or 4.5 ppm) nor treating them with UV-C radiation (1 kJ m-2) systematically and significantly affected their microbial loads during storage. In addition, the assumption that a combination of both treatments could synergistically improve the effect of each treatment could not be verified during this long-term study. In conclusion, microbial load and pathogen development in asparagus spears are highly persistent and, thus, to meet hygienic requirements further investigations will be necessary

Influence of location and fertilization on antioxidant acitivity in highbush blueberries (Vaccinium corymbosum L.)

Highbush blueberry cultivars ‘Bluecrop’ and ‘Reka’ were growing in two variants of mulching and fertilizing systems on formerly used farmland. Fruits were harvested at two picking dates and analyzed for their content of phenolic compounds and antioxidant activity. These data were compared with samples of two forest soil locations from the Brandenburg region (Beelitz and Klaistow). The results showed significant differences between cultivars, both harvest times and different locations. The variations in fertilization and ground cover (with or without mulch) showed significant differences. Moreover, it is demonstrated that without ground cover and commercial fertilization higher contents of total phenolic compounds and an increase in antioxidant activity tendentiously occurred. This result paralleled the decline in vegetative growth and was associated with drought stress

UV-B induced changes of phenol composition and antioxidant activity in black currant fruit (Ribes nigrum L.)

Information on UV-B elicitor mediated changes on phenolic composition and antioxidant activity of black currant (Ribes nigrum L.) are scanty. In the present study physiological ripe black currant fruits were harvested and exposed to UV-B radiation with different exposure and adaptation times. The influence of UV-B on phenolic profile and quantitative composition as well as on the corresponding antioxidant activity was investigated. Antioxidant activity was screened with electron spin resonance spectrometry (ESR), while phenolic compound composition was conducted by HPLC analysis. Total phenol content and phenolic composition (flavonols, anthocyanins, hydroxycinnamic and hydroxybenzoic acids) increased to a large extent during UV-B treatment, irrespective of the adaptation time. Anthocyanins are concluded to absorb UV radiation within a short time, meanwhile flavonols and phenolic acids are assumed to have an impact on antioxidant protection of UV-B mediated tissue damage. Moreover, antioxidant activity significantly correlated with different phenolic compounds and increased to a similar extent by UV-B exposure

Development of a network for the on-farm conservation of crop genetic resources: First results of a pilot project for the re-introduction of old Lactuca varieties to the market

In a pilot project, we examined the chance of maintaining plant genetic resources by commercial utilization of old varieties using Lactuca sativa as a model plant. Nine market gardens in the region of Berlin and Brandenburg cultivated 18 old varieties during four cultivation periods to test field performance. They supplied the products to the market in their customary manner to analyse marketing success. Seven of the market gardens practice organic horticulture. In a complementary field trial at Humboldt-Universität zu Berlin, we established data concerning the field performance of the varieties, analysed dry matter contents, nitrate and phenol concentrations, and observed shelf life for two days under simulated retail conditions (18°C, 80% rel. air humidity). Generally, yield was acceptable for market purposes. However, cultivation in autumn failed because of the cold climate. Biotic and abiotic factors like slugs or hail caused non-specific damages. Specific problems of particular varieties were less important. Based on the results of 2007, the varieties can be put preliminarily into three categories: suitable for on-farm conservation, suitable for home gardens, and varieties with contrasting results depending on the respective market garden.The nitrate concentrations of all varieties were clearly below the EU acceptable limit of 2500 mg/kg fresh weight of lettuce grown in the field. The phenol concentrations varied from 3.3 to 17.2 mg GAE/g dry weight. Generally, the cultivars had a reasonable shelf life of one to two days, however three varieties showed a better storability whereas four other cultivars deteriorated rapidly. Marketing success was good in Berlin City but poor in the countryside of Brandenburg. The regular customers of the market gardens in Berlin who prefer organic food are a promising target group for further stimulation of interest to buy rare crop varieties. The on-farm conservation of old varieties in market gardens requires relatively large quantities of seeds of good quality. However there might arise problems in seed supply as the VERN e.V. was confronted with bottleneck problems. Therefore, we organised a network of interested market gardens who take on maintenance and propagation of individual varieties. The network will be developed in co-operation with the VERN e.V. who will also process the seed as well as organise the exchange of the various varieties within the network. Further, the network will deal with problems concerning maintenance breeding and seed quality

Impact of postharvest UV-C and ozone treatment on textural properties of white asparagus (Asparagus officinalis L.)*

Optimization of postharvest treatments and storage requirements to reduce microbiological spoilage is essential for the food supply chain of asparagus. In this context, Generally Recognized As Safe (GRAS) treatments such as UV-irradiation and washing with ozonated water gain more and more importance. Information on UV-C and ozone as postharvest treatment for quality assurance of white asparagus is scanty. In the present study, asparagus spears were harvested and exposed to the above mentioned treatments and their combination. The infl uence of both postharvest treatments on biomechanical and biochemical textural related cell wall metabolism was investigated. UV-C-irradiation and washing with ozonated water resulted in a slight reduced respiration in white asparagus spears, but increase in spear tissue toughness. Total cell wall compounds were only tendentiously reduced after 4 days of shelf-life at 20 °C by application of aqueous ozone and UV-C. However, the dosages used in this experiment were relatively low and, hence, did not have pronounced effects. Furthermore, the possible mechanism of UV-C and ozone mediated changes in textural related enzyme activities of white asparagus spears have to be investigated in more detail

Phenolic compounds, pectin and antioxidant activity in blueberries (Vaccinium corymbosum L.) influenced by boron and mulch cover

Highbush blueberry cultivars ‘Bluecrop’ and ‘Reka’ were growing in two variants of mulching and fertilizing systems on formerly used farmland. Particular attention of this work was to study the effect of using pine bark as a mulch layer and foliar application with the plant stimulant Wuxal® Ascofol (3% Boron) on selected bioactive compounds (polyphenols and pectins) of blueberry fruits.The results represented a stress-preventive effect of mulch application. Furthermore, these plants exhibited lower calcium content in fruits due to a reduced calcium uptake from the soil. With regard to the bioactive compounds, mulched plants showed a higher content of pectin which was in contrast to the phenolic compounds. They revealed reduced concentrations in fruits accompanied by a lower antioxidant activity. The foliar supply of boron was able to inactivate polyphenols presumably by complex formation and favoured the formation of pectins