Antigenic Diversity of Human Sapoviruses

Correspondence between virus antigenicity and capsid (VP1) genogrouping and genotyping is likely

Experimental methodologies to support aircraft icing analysis

The experimental methodologies are illustrated by graphs, charts and line drawings. Typical ultrasonic echo signals for dry and wet ice growth, ice accretion rates for various tunnel configurations, the experimental configuration for flight tests of the ultrasonic measuring system and heat balance models used to predict ice growth are among the topics that are illustrated and briefly discussed

Experimental measurements of heat transfer from an iced surface during artificial and natural cloud icing conditions

The heat transfer behavior of accreting ice surfaces in natural (flight test) and simulated (wind tunnel) cloud icing conditions were studied. Observations of wet and dry ice growth regimes as measured by ultrasonic pulse echo techniques were made. Observed wet and dry ice growth regimes at the stagnation point of a cylinder were compared with those predicted using a quasi steady state heat balance model. A series of heat transfer coefficients were employed by the model to infer the local heat transfer behavior of the actual ice surfaces. The heat transfer in the stagnation region was generally inferred to be higher in wind tunnel icing tests than in natural flight icing conditions

Ultrasonic techniques for aircraft ice accretion measurement

Results of tests to measure ice growth in natural (flight) and artificial (icing wind tunnel) icing conditions are presented. Ice thickness is measured using an ultrasonic pulse-echo technique. Two icing regimes, wet and dry ice growth, are identified and the unique ultrasonic signal characteristics associated with these different types of ice growth are described. Ultrasonic measurements of ice growth on cylinders and airfoils exposed to artificial and natural icing conditions are presented. An accuracy of plus or minus 0.5 mm is achieved for ice thickness measurement using the pulse-echo technique. The performance of two-probe type ice detectors is compared to the surface mounted ultrasonic system. The ultrasonically measured ice accretion rates and ice surface condition (wet or dry) are used to compare the heat transfer characteristics for flight and icing wind tunnel environments. In general the heat transfer coefficient is inferred to be higher in the wind tunnel environment, not likely due to higher freestream turbulence levels. Finally, preliminary results of tests to measure ice growth on airfoil using an array of ultrasonic transducers are described. Ice profiles obtained during flight in natural icing conditions are shown and compared with mechanical and stereo image measurements

Mutational study of sapovirus expression in insect cells

Human sapovirus (SaV), an agent of human gastroenteritis, cannot be grown in cell culture, but expression of the recombinant capsid protein (rVP1) in a baculovirus expression system results in the formation of virus-like particles (VLPs). In this study we compared the time-course expression of two different SaV rVP1 constructs. One construct had the native sequence (Wt construct), whereas the other had two nucleotide point mutations in which one mutation caused an amino acid substitution and one was silent (MEG-1076 construct). While both constructs formed VLPs morphologically similar to native SaV, Northern blot analysis indicated that the MEG-1076 rVP1 mRNA had increased steady-state levels. Furthermore, Western blot analysis and an antigen enzyme-linked immunosorbent assay showed that the MEG-1076 construct had increased expression levels of rVP1 and yields of VLPs. Interestingly, the position of the mutated residue was strictly conserved residue among other human SaV strains, suggesting an important role for rVP1 expression

Close-up analysis of aircraft ice accretion

Various types of ice formation have been studied by analysis of high magnification video observations. All testing was conducted in the NASA Lewis Icing Research Tunnel (IRT). A faired 8.9 cm (3.5 in.) diameter metal-clad cylinder and a 5.1 (2 in.) aluminum cylinder were observed by close-up and overview video cameras for several wind tunnel conditions. These included close-up grazing angle, close-up side view, as well as overhead and side overview cameras. Still photographs were taken at the end of each spray along with tracings of the subsequent ice shape. While in earlier tests only the stagnation region was observed, the entire area from the stagnation line to the horn region of glaze ice shapes was observed in this test. The modes or horn formation have been identified within the range of conditions observed. In the horn region, Horn Type A ice is formed by 'dry' feather growth into the flow direction and Horn Type B is formed by a 'wet' growth normal to the surface. The feather growth occurs when the freezing fraction is near unity and roughness elements exist to provide an initial growth site

In-flight measurement of ice growth on an airfoil using an array of ultrasonic transducers

Results of preliminary tests to measure ice growth on an airfoil during flight icing conditions are presented. Ultrasonic pulse echo measurements of ice thickness are obtained from an array of eight ultrasonic transducers mounted flush with the leading edge of the airfoil. These thickness measurements are used to document the evolution of the ice shape during the encounter in the form of successive ice profiles. Results from 3 research flights are presented and discussed. The accuracy of the ultrasonic measurements is found to be within 0.5 mm of mechanical and stereo photograph measurements of the ice accretion

Structural analysis of bovine norovirus protruding domain

AbstractWe determined a structure of a bovine (genogroup III, GIII) norovirus capsid protruding (P) domain using X-ray crystallography. The bovine P domain was reminiscent of other norovirus genogroups (GI, GII, GIV, and GV), but closely matched the human GI P domain. We also identified a monoclonal antibody that was capable of binding the five different (GI–GV) P domains. Our data suggests that genetically diverse noroviruses still contain common epitopes

Human Sapovirus in Clams, Japan

Human sapovirus was detected in 4 of 57 clam packages by reverse transcription–PCR and sequence analysis. This represents the first finding of sapovirus contamination in food. Closely matching sequences have been detected in stool specimens from patients with gastroenteritis in Japan, which indicates a possible food-to-human transmission link